Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28192139
Publication Date : //

Vitamin C down-regulate apo(a) expression via Tet2-dependent DNA demethylation in HepG2 cells.


Lipoprotein(a)[Lp(a)] is a risk factor for coronary heart diseases. However, the metabolism of this protein remains poorly understood. Efficient and specific drugs that can decrease high plasma levels of Lp(a) have not been developed yet. Vitamin C is responsible for maintaining the catalytic activity of a group of iron and 2-oxoglutarate (2OG)-dependent dioxygenases and induces the generation of 5-hydroxymethylcytosine (5hmC) via Ten-eleven translocation (Tet) dioxygenases. In addition, It has been reported vitamin C deficiency induces atherosclerosis and increases Lp(a) and apo(a) plasma levels in Lp(a)+ mice. However, the mechanism is still unclear. In this study, we investigated the effects of vitamin C on apo(a) expression and the possible molecular mechanism of vitamin C that influences apolipoprotein(a) [apo(a)] biosynthesis in HepG2 cells. Results showed that vitamin C significantly inhibited the expression and secretion levels of apo(a). Vitamin C can also increase ELK1 expression and hydroxymethylation of ELK1 promoter and the globle DNA in HepG2 cells. In addition, the effects of vitamin C inhibiting the apo(a) expression were attenuated by ELK1siRNA and Tet2siRNA. These results suggested vitamin C down-regulate apo(a) expression via Tet2-dependent DNA demethylation in HepG2 cells.

Authors : Qu Kai , Ma Xiao-Feng , Li Guo-Hua , Zhang Hai , Liu Ya-Mi , Zhang Kai , Zeng Jun-Fa , Lei Jian-Jun , Wei Dang-Heng , Wang Zuo ,

Related products :

Catalog number Product name Quantity
SL100489-HEPG2 Transfection Reagents and Kits, GenJet™ DNA In Vitro Tranfection Reagent for HepG2 cell was pre_optimized for most efficiently transfecting HepG2 cells. 1 ml
HEPG2-500 HepG2 Human Hepatocellular Carcinoma Cells 500,000 cells
28-870 The thyroid hormone (T3) receptors (TRs) are hormone-dependent transcription factors that regulate expression of a variety of specific target genes. TRIP13 specifically interacts with the ligand bindi 0.1 mg
28-338 The thyroid hormone (T3) receptors (TRs) are hormone-dependent transcription factors that regulate expression of a variety of specific target genes. TRIP13 specifically interacts with the ligand bindi 0.1 mg
25-950 GGCX is an enzyme which catalyzes the posttranslational modification of vitamin K-dependent protein. Many of these vitamin K-dependent proteins are involved in coagulation so the function of the encod 0.05 mg
27-388 ERRs, which are coexpressed with ERs in prostatic cells, could regulate cell growth and modulate ER-mediated pathways via interference on ERalpha transcription in prostatic cells. Not only PNRC2 but a 0.1 mg
30-077 ERRs, which are coexpressed with ERs in prostatic cells, could regulate cell growth and modulate ER-mediated pathways via interference on ERalpha transcription in prostatic cells. Not only PNRC2 but a 0.05 mg
29-325 Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. CPNE1 is a calcium-dependent protein that also contains two N-terminal ty 0.1 mg
30-833 Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene is one of several genes that encode a calcium-dependent protein 0.05 mg
30-652 Over expression of PDCD2L attenutates TNF-alpha release in Daudi cells. PDCD2L over-expression restrained proliferation of HEK293T cells. DNA_flow cytometry analysis showed that the over-expression of 0.05 mg
SL100489-HEPG2 TRANSFECTION REAGENTS AND KITS, GENJET™ DNA TRANFECTION REAGENT PRE-OPTIMIZED FOR HEPG2 CELL, EA, 1.0ML 1ML
SL100489-HEPG2 TRANSFECTION REAGENTS AND KITS, GENJET™ DNA TRANFECTION REAGENT PRE-OPTIMIZED FOR HEPG2 CELL, EA, 1.0ML 5x1ML
TET2_MOUSE ELISA Kit FOR Methylcytosine dioxygenase TET2; organism: Mouse; gene name: Tet2 96T
CSB-EL023397HU Human Methylcytosine dioxygenase TET2(TET2) ELISA kit SpeciesHuman 96T
CSB-EL023397MO Mouse Methylcytosine dioxygenase TET2(TET2) ELISA kit SpeciesMouse 96T
EIAAB42005 Homo sapiens,Human,KIAA1546,Methylcytosine dioxygenase TET2,Nbla00191,TET2
EIAAB42006 Kiaa1546,Methylcytosine dioxygenase TET2,Mouse,Mus musculus,Protein Ayu17-449,Tet2
CSB-EL023397HU Human Methylcytosine dioxygenase TET2(TET2) ELISA kit 96T
CSB-EL023397MO Mouse Methylcytosine dioxygenase TET2(TET2) ELISA kit 96T
30-273 SDF4 may regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. 0.1 mg
A1526 TET2 Primary Antibody, TET2, Species: Human Recombinant Protein Source: Rabbit Polyclonal 100ug
TET2 TET2 Gene tet oncogene family member 2
orb13145 Vitamin K-dependent protein C antibody Polyclonal Rabbit polyclonal to Vitamin K-dependent protein C 100
orb13143 Vitamin K-dependent protein S antibody Polyclonal Rabbit polyclonal to Vitamin K-dependent protein S 100
SL100568-HepG2 Transfection Reagents and Kits, GenMute™ siRNA Transfection Reagent, Pre_optimized for Transfecting HepG2 Cell. 1


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur