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Pubmed ID :28408374
Publication Date : //

Expression of Biglycan in First Trimester Chorionic Villous Sampling Placental Samples and Altered Function in Telomerase-Immortalized Microvascular Endothelial Cells.


Biglycan (BGN) has reduced expression in placentae from pregnancies complicated by fetal growth restriction (FGR). We used first trimester placental samples from pregnancies with later small for gestational age (SGA) infants as a surrogate for FGR. The functional consequences of reduced BGN and the downstream targets of were determined. Furthermore, the expression of targets was validated in primary placental endothelial cells isolated from FGR or control pregnancies. APPROACH AND RESULTS: expression was determined using real-time polymerase chain reaction in placental tissues collected during chorionic villous sampling performed at 10 to 12 weeks' gestation from pregnancies that had known clinical outcomes, including SGA. Short-interference RNA reduced expression in telomerase-immortalized microvascular endothelial cells, and the effect on proliferation, angiogenesis, and thrombin generation was determined. An angiogenesis array identified downstream targets of BGN, and their expression in control and FGR primary placental endothelial cells was validated using real-time polymerase chain reaction. Reduced expression was observed in SGA placental tissues. reduction decreased network formation of telomerase-immortalized microvascular endothelial cells but did not affect thrombin generation or cellular proliferation. The array identified target genes, which were further validated: angiopoetin 4 (), platelet-derived growth factor receptor α (), tumor necrosis factor superfamily member 15 (), angiogenin (), serpin family C member 1 (), angiopoietin 2 (), and CXC motif chemokine 12 () in telomerase-immortalized microvascular endothelial cells and primary placental endothelial cells obtained from control and FGR pregnancies.

Authors : Chui Amy , Gunatillake Tilini , Brennecke Shaun P , Ignjatovic Vera , Monagle Paul T , Whitelock John M , van Zanten Dagmar E , Eijsink Jasper , Wang Yao , Deane James , Borg Anthony J , Stevenson Janet , Erwich Jan Jaap , Said Joanne M , Murthi Padma ,

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