Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28456988
Publication Date : 2017/04/30

Gelatin Zymography Using Leupeptin for the Detection of Various Cathepsin L Forms.


Zymography is a highly sensitive method to assess the activities as well as molecular weights of enzymes in crude biological fluids and tissue extracts. Cathepsin L is a lysosomal cysteine proteinase that is optimally active at slightly acidic pH and is highly unstable in alkaline solutions such as electrode buffer (pH 8.3). Large amounts of cathepsin L are secreted by various cancer cells, where it promotes invasion and metastasis. Leupeptin is a tight-binding inhibitor of cysteine proteinases, and its complex with cathepsin L is stable in alkaline solutions. Moreover, leupeptin can be easily removed from the complex because it is a reversibly binding inhibitor. In addition, leupeptin is too small to influence the electrode migration distance of the complex with cathepsin L on a sodium dodecyl sulfate-polyacrylamide gel. Here, a novel gelatin zymography technique that employs leupeptin to detect pro-, intermediate, and mature cathepsin L forms on the basis of their gelatinolytic activities is described. Further, the differences in the glycosylation, phosphorylation, and processing statuses of lysosomal and secreted cathepsin L forms isolated from cultured HT 1080 cells are demonstrated using this method.

Authors : Hashimoto Yoko ,

Related products :

Catalog number Product name Quantity
BW296 Nutrient Gelatin USE : For recommended by BIS for detection of gelatin liquefaction by proteolytic microorganisms. 5x500gm
BW296 Nutrient Gelatin USE For recommended by BIS for detection of gelatin liquefaction by proteolytic microorganisms. Qty per Litre of Medium: 158
B296 Nutrient Gelatin USE : For detection of gelatin liquefaction by proteolytic microorganisms. 5x500gm
B296 Nutrient Gelatin USE For detection of gelatin liquefaction by proteolytic microorganisms. Qty per Litre of Medium: 128
TM 1122 GELATIN IRON AGAR (for detection of gelatin liqu 500 gm
TM 1122 GELATIN IRON AGAR (for detection of gelatin liqu 500 gm
147385-61-3 leupeptin trifluoroacetate salt 90+ percent leupeptin trifluoroacetat 1g
TM 1059 NUTRIENT GELATIN (as per BIS), (for detection of 500 gm
TM 1059 NUTRIENT GELATIN (as per BIS), (for detection of 500 gm
TM 1059 NUTRIENT GELATIN (as per BIS), (for detection of 100 gm
TM 1059 NUTRIENT GELATIN (as per BIS), (for detection of 100 gm
TM 241 NUTRIENT GELATIN (for detection of gelatine liquefaction 500 gm
TM 241 NUTRIENT GELATIN (for detection of gelatine liquefaction 500 gm
24365-46-6 Leupeptin Leupeptin _ CH85402 1g
TM 148 LACTOSE GELATIN MEDIUM (for detection of Clostridium species from food samples) 500 gm
TM 1192 GELATIN PHOSPHATE BUFFER (for detection of toxin in food products suspecte 100 gm
TM 1192 GELATIN PHOSPHATE BUFFER (for detection of toxin in food products suspecte 500 gm
TM 1192 GELATIN PHOSPHATE BUFFER (for detection of toxin in food products suspecte 500 gm
TM 1192 GELATIN PHOSPHATE BUFFER (for detection of toxin in food products suspecte 100 gm
B1196 Lactose Gelatin Medium USE For detection of Clostridium species from food samples. Qty per Litre of Medium: 135
B1196 Lactose Gelatin Medium USE : For detection of Clostridium species from food samples. 5x500gm
TM 149 LACTOSE GELATIN MEDIUM, MODIFIED (AOAC) (for detection and presumptive identification of Clostridium perfringe 500 gm
TM 149 LACTOSE GELATIN MEDIUM, MODIFIED (AOAC) (for detection and presumptive identification of Clostridium perfringe 500 gm
B1183 Gelatin Phosphate Buffer USE For toxin detection in food products when Clostridium botulinum is suspected. Qty per Litre of Medium: 6
B1183 Gelatin Phosphate Buffer USE : For toxin detection in food products when Clostridium botulinum is suspected. 5x500gm


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur