Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28630423
Publication Date : //

Thymosin beta-4 regulates activation of hepatic stellate cells via hedgehog signaling.


The molecular mechanisms of thymosin beta-4 (TB4) involved in regulating hepatic stellate cell (HSC) functions remain unclear. Therefore, we hypothesize that TB4 influences HSC activation through hedgehog (Hh) pathway. HSC functions declined in a TB4 siRNA-treated LX-2. TB4 suppression down-regulated both integrin linked kinase (ILK), an activator of smoothened, and phosphorylated glycogen synthase kinase 3 beta (pGSK-3B), an inactive form of GSK-3B degrading glioblastoma 2 (GLI2), followed by the decreased expression of both smoothened and GLI2. A TB4 CRISPR also blocked the activation of primary HSCs, with decreased expression of smoothened, GLI2 and ILK compared with cells transfected with nontargeting control CRISPR. Double immunostaining and an immunoprecipitation assay revealed that TB4 interacted with either smoothened at the cytoplasm or GLI2 at the nucleus in LX-2. Smoothened suppression in primary HSCs using a Hh antagonist or adenovirus transduction decreased TB4 expression with the reduced activation of HSCs. Tb4-overexpressing transgenic mice treated with CCl were susceptible to the development hepatic fibrosis with higher levels of ILK, pGSK3b, and Hh activity, as compared with wild-type mice. These findings demonstrate that TB4 regulates HSC activation by influencing the activity of Smoothened and GLI2, suggesting TB4 as a novel therapeutic target in liver disease.

Authors : Kim Jieun , Hyun Jeongeun , Wang Sihyung , Lee Chanbin , Lee Jae-Wook , Moon Eun-Yi , Cha Heejae , Diehl Anna Mae , Jung Youngmi ,

Related products :

Catalog number Product name Quantity
P10623 Mouse Hepatic Stellate Cells 5 x 10A5 cells/vial
5-10011 Rat hepatic stellate cells, 3-week Wistar rat vial
26-210 LAX1 is a single-pass type III membrane protein. It negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and BCR (B-cell antigen receptor)-mediated signaling in B-cells. 0.05 mg
EIAAB10269 Cygb,Cytoglobin,HGb,Histoglobin,Rat,Rattus norvegicus,Stap,Stellate cell activation-associated protein
EIAAB10270 CYGB,Cytoglobin,HGb,Histoglobin,Homo sapiens,Human,STAP,Stellate cell activation-associated protein
EIAAB41412 Homo sapiens,Human,NB thymosin beta,Thymosin beta-15A,Thymosin-like protein 8,TMSB15A,TMSL8,TMSNB
EIAAB28010 Lab,Lat2,Linker for activation of B-cells,Linker for activation of T-cells family member 2,Membrane-associated adapter molecule,Mouse,Mus musculus,Non-T-cell activation linker,Ntal,Wbscr15,Wbscr5,Will
EIAAB28012 Homo sapiens,HSPC046,Human,LAB,LAT2,Linker for activation of B-cells,Linker for activation of T-cells family member 2,Membrane-associated adapter molecule,Non-T-cell activation linker,NTAL,WBS15,WBSCR
EIAAB28013 Lab,Lat2,Linker for activation of B-cells,Linker for activation of T-cells family member 2,Non-T-cell activation linker,Ntal,Rat,Rattus norvegicus,Wbscr5
28-565 The KCTD11 gene encodes a protein that has been identified as a suppressor of Hedgehog signaling. Its inactivation might lead to a deregulation of the tumor-promoting Hedgehog pathway in medulloblasto 0.1 mg
28-319 The KCTD11 gene encodes a protein that has been identified as a suppressor of Hedgehog signaling. Its inactivation might lead to a deregulation of the tumor-promoting Hedgehog pathway in medulloblasto 0.1 mg
orb82714 Human IL1 beta protein IL1 beta is a potent immuno-modulator that mediates a wide range of immune andinflammatory responses including the activation of B- and T-cells. Recombinant HumanIL1 beta contai 1 mg
29-852 DTX2 is a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. DTX2 probably acts both as a positive and 0.05 mg
K868-6 DiscoveryPak™ Hedgehog (Hh) Signaling Pathway Inhibitors Set Hedgehog (Hh) Signaling Pathway Inhibitors Set Inhibitors
EIAAB28011 Chicken,Gallus gallus,LAB,LAT2,Linker for activation of B-cells,Linker for activation of T-cells family member 2,Non-T-cell activation linker,NTAL
30-651 NRIP2 down-regulates transcriptional activation by nuclear receptors such as NR1F2 0.05 mg
30-561 TRAF3IP3 may function as an adapter molecule that regulates TRAF3-mediated JNK activation. 0.05 mg
E1782b ELISA kit Bos taurus,Bovine,Thymosin beta-10,Thymosin beta-9,TMSB10 96T
U1782b CLIA Bos taurus,Bovine,Thymosin beta-10,Thymosin beta-9,TMSB10 96T
E1782b ELISA Bos taurus,Bovine,Thymosin beta-10,Thymosin beta-9,TMSB10 96T
orb84821 IL4 protein Interleukin4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone ma 10
orb82806 Mouse IL4 protein Interleukin-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and 1 mg
orb84786 IL4 protein Interleukin4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone ma 20
30-436 Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells 0.1 mg
30-435 Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells 0.05 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur