Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28655761
Publication Date : //

The resolution of acute inflammation induced by cyclic AMP is dependent on annexin A1.


Annexin A1 (AnxA1) is a glucocorticoid-regulated protein known for its anti-inflammatory and pro-resolving effects. We have shown previously that the cAMP-enhancing compounds rolipram (ROL; a PDE4 inhibitor) and Bt2cAMP (a cAMP mimetic) drive caspase-dependent resolution of neutrophilic inflammation. In this follow-up study, we investigated whether AnxA1 could be involved in the pro-resolving properties of these compounds using a model of LPS-induced inflammation in BALB/c mice. The treatment with ROL or Bt2cAMP at the peak of inflammation shortened resolution intervals, improved resolution indices, and increased AnxA1 expression. In vitro studies showed that ROL and Bt2cAMP induced AnxA1 expression and phosphorylation, and this effect was prevented by PKA inhibitors, suggesting the involvement of PKA in ROL-induced AnxA1 expression. Akin to these in vitro findings, H89 prevented ROL- and Bt2cAMP-induced resolution of inflammation, and it was associated with decreased levels of intact AnxA1. Moreover, two different strategies to block the AnxA1 pathway (by using N-t-Boc-Met-Leu-Phe, a nonselective AnxA1 receptor antagonist, or by using an anti-AnxA1 neutralizing antiserum) prevented ROL- and Bt2cAMP-induced resolution and neutrophil apoptosis. Likewise, the ability of ROL or Bt2cAMP to induce neutrophil apoptosis was impaired in AnxA-knock-out mice. Finally, in in vitro settings, ROL and Bt2cAMP overrode the survival-inducing effect of LPS in human neutrophils in an AnxA1-dependent manner. Our results show that AnxA1 is at least one of the endogenous determinants mediating the pro-resolving properties of cAMP-elevating agents and cAMP-mimetic drugs.

Authors : Lima Kátia M , Vago Juliana P , Caux Thaís R , Negreiros-Lima Graziele Letícia , Sugimoto Michelle A , Tavares Luciana P , Arribada Raquel G , Carmo Aline Alves F , Galvão Izabela , Costa Bruno Rocha C , Soriani Frederico M , Pinho Vanessa , Solito Egle , Perretti Mauro , Teixeira Mauro M , Sousa Lirlândia P ,

Related products :

Catalog number Product name Quantity
28-520 Annexin A7 is a member of the annexin family of calcium-dependent phospholipid binding proteins. The Annexin A7 gene contains 14 exons and spans approximately 34 kb of DNA. Structural analysis of the 0.1 mg
10-664-50002 Annexin A3 - Annexin III; Lipocortin III; Placental anticoagulant protein III; PAP-III; 35-alpha calcimedin; Inositol 1.2-cyclic phosphate 2-phosphohydrolase N_A 0.05 mg
18-003-42795 Annexin A3 - Annexin III; Lipocortin III; Placental anticoagulant protein III; PAP-III; 35-alpha calcimedin; Inositol 1.2-cyclic phosphate 2-phosphohydrolase Polyclonal 0.1 mg Protein A
18-003-42794 Annexin A3 - Annexin III; Lipocortin III; Placental anticoagulant protein III; PAP-III; 35-alpha calcimedin; Inositol 1.2-cyclic phosphate 2-phosphohydrolase Polyclonal 0.1 mg Protein A
25-366 Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have b 0.05 mg
28-552 Annexin A4 (ANXA4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have 0.1 mg
LF-PA42581 Anti-Cyclic GMP-Dependent Protein Kinase(PRKG), Rabbit Polyclonal to Cyclic GMP-Dependent Protein Kinase(PRKG), Isotype , Host Rabbit 50
EIAAB30305 cGMP-dependent 3',5'-cyclic phosphodiesterase,cGSPDE,CGS-PDE,Cyclic GMP-stimulated phosphodiesterase,Homo sapiens,Human,PDE2A
EIAAB30304 cGMP-dependent 3',5'-cyclic phosphodiesterase,cGSPDE,CGS-PDE,Cyclic GMP-stimulated phosphodiesterase,Mouse,Mus musculus,Pde2a
EIAAB30306 Bos taurus,Bovine,cGMP-dependent 3',5'-cyclic phosphodiesterase,cGSPDE,CGS-PDE,Cyclic GMP-stimulated phosphodiesterase,PDE2A
EIAAB30303 cGMP-dependent 3',5'-cyclic phosphodiesterase,cGSPDE,CGS-PDE,Cyclic GMP-stimulated phosphodiesterase,Pde2a,Rat,Rattus norvegicus
EIAAB43494 Homo sapiens,Human,Inflammation factor protein 20,TIPE2,TNF alpha-induced protein 8-like protein 2,TNFAIP8L2,TNFAIP8-like protein 2,Tumor necrosis factor alpha-induced protein 8-like protein 2
28-553 ANXA5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 0.1 mg
28-519 ANXA7 is a member of the annexin family of calcium-dependent phospholipid binding proteins. The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced casset 0.1 mg
30-094 Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and s 0.1 mg
28-517 Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been 0.1 mg
28-518 Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been 0.1 mg
18-003-44064 Cyclic AMP-dependent transcription factor ATF-4 - Activating transcription factor 4; DNA-binding protein TAXREB67; Cyclic AMP response element-binding protein 2; CREB2 Polyclonal 0.1 mg Protein A
E1346h ELISA kit Activating transcription factor 1,ATF1,cAMP-dependent transcription factor ATF-1,Cyclic AMP-dependent transcription factor ATF-1,Homo sapiens,Human,Protein TREB36 96T
U1346h CLIA Activating transcription factor 1,ATF1,cAMP-dependent transcription factor ATF-1,Cyclic AMP-dependent transcription factor ATF-1,Homo sapiens,Human,Protein TREB36 96T
E1346h ELISA Activating transcription factor 1,ATF1,cAMP-dependent transcription factor ATF-1,Cyclic AMP-dependent transcription factor ATF-1,Homo sapiens,Human,Protein TREB36 96T
ER685 Cyclic AMP-dependent transcription factor ATF-2 Elisa Kit 96T
ATF5_RAT Rat ELISA Kit FOR Cyclic AMP-dependent transcription factor ATF-5 96T
E13842486 Rat Cyclic AMP-dependent transcription factor ATF-2 (ATF-2 ) ELISA Kit 1
EIAAB37147 Rat,Rattus norvegicus,Renal osmotic stress-induced Na-Cl organic solute cotransporter,ROSIT,Slc6a18,Sodium- and chloride-dependent transporter XTRP2,Sodium-dependent neutral amino acid transporter B(0


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur