Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28686599
Publication Date : //

Flux balance analysis predicts Warburg-like effects of mouse hepatocyte deficient in miR-122a.


The liver is a vital organ involving in various major metabolic functions in human body. MicroRNA-122 (miR-122) plays an important role in the regulation of liver metabolism, but its intrinsic physiological functions require further clarification. This study integrated the genome-scale metabolic model of hepatocytes and mouse experimental data with germline deletion of Mir122a (Mir122a-/-) to infer Warburg-like effects. Elevated expression of MiR-122a target genes in Mir122a-/-mice, especially those encoding for metabolic enzymes, was applied to analyze the flux distributions of the genome-scale metabolic model in normal and deficient states. By definition of the similarity ratio, we compared the flux fold change of the genome-scale metabolic model computational results and metabolomic profiling data measured through a liquid-chromatography with mass spectrometer, respectively, for hepatocytes of 2-month-old mice in normal and deficient states. The Ddc gene demonstrated the highest similarity ratio of 95% to the biological hypothesis of the Warburg effect, and similarity of 75% to the experimental observation. We also used 2, 6, and 11 months of mir-122 knockout mice liver cell to examined the expression pattern of DDC in the knockout mice livers to show upregulated profiles of DDC from the data. Furthermore, through a bioinformatics (LINCS program) prediction, BTK inhibitors and withaferin A could downregulate DDC expression, suggesting that such drugs could potentially alter the early events of metabolomics of liver cancer cells.

Authors : Wu Hua-Qing , Cheng Mei-Ling , Lai Jin-Mei , Wu Hsuan-Hui , Chen Meng-Chun , Liu Wen-Huan , Wu Wu-Hsiung , Chang Peter Mu-Hsin , Huang Chi-Ying F , Tsou Ann-Ping , Shiao Ming-Shi , Wang Feng-Sheng ,

Related products :

Catalog number Product name Quantity
30-204 Retinoids exert biologic effects such as potent growth inhibitory and cell differentiation activities and are used in the treatment of hyperproliferative dermatological diseases. These effects are med 0.1 mg
MZIP122a-PA-1 MicroRNA Tools miRZip-122a anti-miR-122a microRNA construct Bacterial Streak
orb60893 Matrine((+)-Matrine) Matrine((+)-Matrine) is an alkaloid found in plants from the Sophora family. It has a variety of pharmacological effects, including anti-cancer effects, and action as a kappa opio 50 mg
26-815 Strong TKTL1 protein expression has been correlated with a certain type of glucose metabolism (aerobic glycolysis; Warburg effect) and to cells which are affected by chronic complications of diabetic 0.05 mg
RANT-122A Mouse Anti Human Interferon_alpha Neutralizing 500 µg
CSB-EL008032MO Mouse family with sequence similarity 122A (FAM122A) ELISA kit, Species Mouse, Sample Type serum, plasma 96T
RANT-122A IFN_a Neut Mouse Anti Human Interferon_alpha Neutralizing 500µg
TotalLabQuant Base package*: 1D image analysis plus colony counting and array analysis
XGC-BiRDS-MeDIP-Seq Bioinformatics analysis of Methylome data for Methylation analysis Sample
AS-MRA-P8 MARIA™ ANALYSIS SERVICE 8-plex analysis Environmental Testing Services 1 sample
AS-MRA-P5 MARIA™ ANALYSIS SERVICE 5-plex analysis Environmental Testing Services 1 sample
TotalLab Quant Base package* 1D image analysis plus colony counting and array analysis
XGC-BiRDS-WGBS Bioinformatics analysis of Whole Genome Bisulphite Sequencing data for Methylation analysis Sample
TotalLab Quant Base package* 1D image analysis plus colony counting and array analysis 1
Phoretix1D Base package*: 1D image analysis with band pattern matching, plus colony counting and array analysis
Phoretix 1D  Base package* 1D image analysis with band pattern matching, plus colony counting and array analysis
Phoretix 1D Base package* 1D image analysis with band pattern matching, plus colony counting and array analysis 1
MINIBAL Mini balance 60g mini balance (0.01g)
25-758 SCGN is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and c 0.05 mg
AS-MRA-P5 8-plex analysis MARIA™ ANALYSIS SERVICE 1 sample
AS-MRA-P8 8-plex analysis MARIA™ ANALYSIS SERVICE 1 sample
1601 microRNA profiling analysis full service-RNA labeling and processing, array hybridization assay and process, data output, data analysis and report 1
SPC-122A Calreticulin 50ul
K 122A Avian Influenza virus (H9, H5, N1) 100 reactions
HP-0048 miR-122a(HS) miRNA probe 10 Reactions


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur