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Pubmed ID :28808849
Publication Date : //

A rapid method for establishment of a reverse genetics system for canine parvovirus.


Canine parvovirus (CPV) is an important and highly prevalent pathogen of dogs that causes acute hemorrhagic enteritis disease. Here, we describe a rapid method for the construction and characterization of a full-length infectious clone (rCPV) of CPV. Feline kidney (F81) cells were transfected with rCPV incorporating an engineered EcoR I site that served as a genetic marker. The rescued virus was indistinguishable from that of wild-type virus in its biological properties.

Authors : Yu Yongle , Su Jun , Wang Jigui , Xi Ji , Mao Yaping , Hou Qiang , Zhang Xiaomei , Liu Weiquan ,

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