Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28831172
Publication Date : 2017/08/23

Pioglitazone Enhances Cytosolic Lipolysis, β-oxidation and Autophagy to Ameliorate Hepatic Steatosis.


Non-alcoholic fatty liver disease closely contributes to the development of obesity and insulin resistance. Even though pioglitazone has been reported to effectively lessen hepatic steatosis in human studies, its molecular mechanism remains unclear. This study is designed to investigate the regulation of cytosolic lipolysis, β-oxidation and autophagy by pioglitazone in a mice model of high fat diet (HFD) and cell model incubated with palmitic acid. Our results revealed hepatic steatosis was apparently induced by HFD and it was significantly reversed by pioglitazone. The serum insulin and hepatic triglyceride content was significantly decreased by co-administered pioglitazone with HFD. Hepatic expression of cytosolic-lipolysis related proteins (ATGL, HSL), β-oxidation (CPT-1A) and autophagy-related proteins (ATG7, LC3, LAL) was significantly enhanced by pioglitazone. Knockdown PPARα/PPARγ in AML12 cells significantly and proportionally reduced the expressions of ATGL, CPT-1A and LC3II, which was induced by pioglitazone. Furthermore, facilitation of the autophagic flux by pioglitazone was obviously blocked by lysosomal inhibitor, leupeptin, to demonstrate accumulation of the LC3II and intracellular lipid in AML12 cells. Our results demonstrated that pioglitazone attenuating the hepatic steatosis may be mediated by enhancing cytosolic lipolysis, β-oxidation and autophagy in a PPARα and PPARγ dependent manner.

Authors : Hsiao Pi-Jung , Chiou Hsin-Ying Clair , Jiang He-Jiun , Lee Mei-Yueh , Hsieh Tusty-Jiuan , Kuo Kung-Kai ,

Related products :

Catalog number Product name Quantity
28-647 Pycard is a cytosolic soluble protein that forms insoluble aggregates and enhances etoposide-induced apoptosis. 0.1 mg
112529-15-4 Pioglitazone hydrochloride Pioglitazone HCl 1g
orb62241 Pioglitazone Pioglitazone For research use only. 500 g
E925750 N-[Ethyl-(2-pyridyl-5-ethyl) Pioglitazone (Pioglitazone Impurity) C28H31N3O3S CAS: 952188-00-0 2.5 mg
30-958 Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation ATG 0.05 mg
KA1380 Steatosis Colorimetric Assay Kit 1 Kit
111025-46-8 Pioglitazone Pioglitazone 1g
12529-15-4 Pioglitazone HCl Pioglitazone HCl 1g
11025-46-8 Pioglitazone base (B) PIOGLITAZONE BASE 1g
26-923 HADH functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-len 0.05 mg
HOM284 Hepatic XXX Homogenate Hepatic S9 fractions
26-750 TMEM74 plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction. 0.05 mg
Autophagy Autophagy ,AT1H2:2 SignArrays
28-092 Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during diff 0.1 mg
28-091 Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during diff 0.05 mg
25-796 Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during diff 0.05 mg
GWB-7D3626 Anti- TCF2 (transcription factor 2. hepatic; LF-B3; variant hepatic nuclear factor) Antibody
GWB-9702DA Anti- TCF2 (transcription factor 2. hepatic; LF-B3; variant hepatic nuclear factor) Antibody
orb80926 Human Autophagy Related 5 protein Autophagy Protein Human Recombinant produced in E.coli is non-glycosylated, polypeptide chain having molecular weight of 31.6 kDa. APG5 contains T7 tag at N-terminus 2
EIAAB42248 Acat2,Acetyl-CoA acetyltransferase, cytosolic,Cytosolic acetoacetyl-CoA thiolase,Mouse,Mus musculus
EIAAB42249 Acat2,Acetyl-CoA acetyltransferase, cytosolic,Cytosolic acetoacetyl-CoA thiolase,Rat,Rattus norvegicus
E0769h ELISA Hepatic lipase,Hepatic triacylglycerol lipase,HL,Homo sapiens,HTGL,Human,Lipase member C,LIPC 96T
U0769h CLIA Hepatic lipase,Hepatic triacylglycerol lipase,HL,Homo sapiens,HTGL,Human,Lipase member C,LIPC 96T
E0769h ELISA kit Hepatic lipase,Hepatic triacylglycerol lipase,HL,Homo sapiens,HTGL,Human,Lipase member C,LIPC 96T
E0769Rb ELISA kit Hepatic lipase,Hepatic triacylglycerol lipase,HL,Lipase member C,LIPC,Oryctolagus cuniculus,Rabbit 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur