GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene.

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28831252
Publication Date : //

Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene.

Various strategies have been utilized to improve both gene transfer efficiency and cell-induced toxicity of polyethylenimine (PEI), the most extensively investigated cationic polymeric vector. In this study, we sought to enhance transfection efficiency of low molecular weight PEI (LMW PEI) while maintaining its low toxicity by cross-linking LMW PEI via succinic acid linker. These modifications were designed to improve the hydrophilic-hydrophobic balance of the polymer, by enhancing the buffering capacity and maintaining low cytotoxic effects of the final conjugate. Decreased expression of CD200 in the central nervous system has been considered as one of the proposed mechanisms associated with neuroinflammation in multiple sclerosis; therefore, we selected plasmid-encoding CD200 gene for transfection using the modified PEI derivatives. Dynamic light scattering experiments demonstrated that the modified PEIs were able to condense plasmid DNA and form polyplexes with a size of approximately 130 nm. The highest level of CD200 expression was achieved at a carrier to plasmid ratio of 8, where the expression level was increased by 1.5 fold in the SH-SY5Y cell line, an in vitro model of neurodegenerative disorders. Furthermore, the results of in vivo imaging of the LMW PEI-based nanoparticles in the mouse model of multiple sclerosis revealed that fluorescently labeled plasmid encoding CD200 was distributed from the injection site to various tissues and organs including lymph nodes, liver, brain, and finally, kidneys. The nanoparticles also showed the ability to cross the blood-brain barrier and enter the periventricular area.

Authors : Nouri Fatemeh , Sadeghpour Hossein , Heidari Reza , Dehshahri Ali ,

Related products :

Catalog number	Product name	Quantity

SL100573	Transfection Reagents and Kits, GenJet™ Transfection Buffer was formulated for GenJet™ siRNA Transfection Reagent for optimal transfection efficiency and maximum gene silencing.	8 ml
SL100575	Transfection Reagents and Kits, PepMute™ Transfection Buffer was formulated for PepMute™ siRNA Transfection Reagent for optimal transfection efficiency and maximum gene silencing.	8 ml
SL100575	Transfection Reagents and Kits, PepMute™ Transfection Buffer was formulated for PepMute™ siRNA Transfection Reagent for optimal transfection efficiency and maximum gene silencing.	4 ml
SL100574	Transfection Reagents and Kits, Powerfect™ Transfection Buffer was formulated for Powerfect™ siRNA Transfection Reagent for optimal transfection efficiency and maximum gene silencing.	8 ml
SL100574	Transfection Reagents and Kits, Powerfect™ Transfection Buffer was formulated for Powerfect™ siRNA Transfection Reagent for optimal transfection efficiency and maximum gene silencing.	4 ml
SL100573	Transfection Reagents and Kits, GenJet™ Transfection Buffer was formulated for GenJet™ siRNA Transfection Reagent for optimal transfection efficiency and maximum gene silencing.	4 ml
SL100569	Transfection Reagents and Kits, Powerfect™ siRNA Transfection Reagent is one of the most powerful liposome based tool for in vitro RNA delivery.	0,5 ml
SL100569	Transfection Reagents and Kits, Powerfect™ siRNA Transfection Reagent is one of the most powerful liposome based tool for in vitro RNA delivery.	0,1 ml
SL100569	Transfection Reagents and Kits, Powerfect™ siRNA Transfection Reagent is one of the most powerful liposome based tool for in vitro RNA delivery.	1 ml
SL100570	Transfection Reagents and Kits, GenJet™ siRNA Transfection Reagent, formulated by PDCC technology, ensures superior efficiency siRNA delivery with invisible cytotoxicity.	0,1 ml
SL100570	Transfection Reagents and Kits, GenJet™ siRNA Transfection Reagent, formulated by PDCC technology, ensures superior efficiency siRNA delivery with invisible cytotoxicity.	0,5 ml
SL100570	Transfection Reagents and Kits, GenJet™ siRNA Transfection Reagent, formulated by PDCC technology, ensures superior efficiency siRNA delivery with invisible cytotoxicity.	1 ml
SL100566	Transfection Reagents and Kits, PepMute™ siRNA Transfection Reagent is a novel small peptide based siRNA delivery tool.	1 ml
SL100566	Transfection Reagents and Kits, PepMute™ siRNA Transfection Reagent is a novel small peptide based siRNA delivery tool.	0,1 ml
SL100566	Transfection Reagents and Kits, PepMute™ siRNA Transfection Reagent is a novel small peptide based siRNA delivery tool.	0,5 ml
SL100571	Transfection Reagents and Kits, PepMute™ Plus siRNA Transfection Reagent is a novel small peptide based siRNA delivery tool.	0,1 ml
SL100571	Transfection Reagents and Kits, PepMute™ Plus siRNA Transfection Reagent is a novel small peptide based siRNA delivery tool.	0,5 ml
SL100571	Transfection Reagents and Kits, PepMute™ Plus siRNA Transfection Reagent is a novel small peptide based siRNA delivery tool.	1 ml
SL100572	Transfection Reagents and Kits, GenMute™ Transfection Buffer was formulated for diluting GenMute™ siRNA Transfection Reagent and siRNA for optimal transfection efficiency and maximum knockdown.	4 ml
SL100572	Transfection Reagents and Kits, GenMute™ Transfection Buffer was formulated for diluting GenMute™ siRNA Transfection Reagent and siRNA for optimal transfection efficiency and maximum knockdown.	8 ml
28-974	This gene was identified based on its homology to the gene encoding the RING3 protein, a serine_threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility c	0.1 mg
25-204	BRD3 gene was identified based on its homology to the gene encoding the RING3 protein, a serine_threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility c	0.05 mg
orb80575	Human CD4 (125-202) protein CD-4 Human Recombinant is fused with 4kDa His Tag and encoding amino acids 125-202, having total molecular weight of 19 kDa. For research use only.	5
SL100668	Transfection Reagents and Kits, LipoD293™ In Vitro DNA Transfection Reagent (Ver. II), a cationic liposome based transfection reagent, was developed by our innovative and proprietary lipid conjugation	0,5 ml
SL100668	Transfection Reagents and Kits, LipoD293™ In Vitro DNA Transfection Reagent (Ver. II), a cationic liposome based transfection reagent, was developed by our innovative and proprietary lipid conjugation	1 ml

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel: 0208-080893 Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62 SWIFT RABONL2U

GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur

ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF