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Pubmed ID :28912106
Publication Date : //

Vascular activation of Kchannels and Na-KATPase activity of estrogen-deficient female rats.


The goal of the present study was to evaluate vascular potassium channels and Na-K-ATPase activity in estrogen deficient female rats. Female rats that underwent ovariectomy were assigned to receive daily treatment with placebo (OVX) or estrogen replacement (OVX+E2, 1mg/kg, once a week, i.m.). Aortic rings were used to examine the involvement of Kchannels and Na-K-ATPase in vascular reactivity. Acetylcholine (ACh)-induced relaxation was analyzed in the presence of L-NAME (100μM) and Kchannels blockers: tetraethylammonium (TEA, 5mM), 4-aminopyridine (4-AP, 5mM), iberiotoxin (IbTX, 30nM), apamin (0.5mM), charybdotoxin (ChTX, 0.1mM) and iberiotoxin plus apamin. When aortic rings were pre-contracted with KCl (60mM) or pre-incubated with TEA (5mM), 4-aminopyridine (4-AP, 5mM) and iberiotoxin (IbTX, 30nM) plus apamin (0.5μM), the ACh-induced relaxation was less effective in the ovariectomized group. Additionally, 4-AP and IbTX decreased the relaxation by sodium nitroprusside in all groups but this reduction was greater in the ovariectomized group. Estrogen deficiency also increased aortic functional Na-KATPase activity evaluated by K-induced relaxation. L-NAME or endothelium removal were not able to block the increase in aortic functional Na-KATPase activity, however, TEA (5mM) restored this increase to the control level. We also found that estrogen deficiency increased superoxide anion production and reduced nitric oxide release in aortic ring from ovariectomized animals. In summary, our results emphasize that the process underlying ACh-induced relaxation is preserved in ovariectomized animals due to the activation of Kchannels and increased Na-KATPase activity.

Authors : Ribeiro Junior Rogério Faustino , Fiorim Jonaina , Marques Vinicius Bermond , de Sousa Ronconi Karoline , Botelho Tatiani , Grando Marcella D , Bendhack Lusiane M , Vassallo Dalton Valentim , Stefanon Ivanita ,

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