Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28915615
Publication Date : 2017/09/16

Expression of CD38 in myeloma bone niche: A rational basis for the use of anti-CD38 immunotherapy to inhibit osteoclast formation.


It is known that multiple myeloma (MM) cells express CD38 and that a recently developed human anti-CD38 monoclonal antibody Daratumumab mediates myeloma killing. However, the expression of CD38 and other functionally related ectoenzymes within the MM bone niche and the potential effects of Daratumumab on bone cells are still unknown. This study firstly defines by flow cytometry and immunohistochemistry the expression of CD38 by bone marrow cells in a cohort of patients with MM and indolent monoclonal gammopathies. Results indicate that only plasma cells expressed CD38 at high level within the bone niche. In addition, the flow cytometry analysis shows that CD38 was also expressed by monocytes and early osteoclast progenitors but not by osteoblasts and mature osteoclasts. Indeed, CD38 was lost during in vitro osteoclastogenesis. Consistently, we found that Daratumumab reacted with CD38 expressed on monocytes and its binding inhibited in vitro osteoclastogenesis and bone resorption activity from bone marrow total mononuclear cells of MM patients, targeting early osteoclast progenitors. The inhibitory effect was not observed from purified CD14(+) cells, suggesting an indirect inhibitory effect of Daratumumab. Interestingly, all-trans retinoic acid treatment increased the inhibitory effect of Daratumumab on osteoclast formation. These observations provide a rationale for the use of an anti-CD38 antibody-based approach as treatment for multiple myeloma-induced osteoclastogenesis.

Authors : Costa Federica , Toscani Denise , Chillemi Antonella , Quarona Valeria , Bolzoni Marina , Marchica Valentina , Vescovini Rosanna , Mancini Cristina , Martella Eugenia , Campanini Nicoletta , Schifano Chiara , Bonomini Sabrina , Accardi Fabrizio , Horenstein Alberto L , Aversa Franco , Malavasi Fabio , Giuliani Nicola ,

Related products :

Catalog number Product name Quantity
20-272-190861 CD38 - Mouse monoclonal [HI157] to CD38; EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; Lymphocyte differentiation antigen CD38; T10; Acute lymphoblastic leukemia cells antigen CD38 Mon 50 TESTS
20-272-192413 CD38 - Mouse monoclonal [HIT2] to CD38; EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; Lymphocyte differentiation antigen CD38; T10; Acute lymphoblastic leukemia cells antigen CD38 Mono 0.1 mg
101-M306 CD38, Host: Mouse, Species: Anti-Human, Synonyms: CD38; T10 100 ug
GWB-F922F0 CD38 Antigen (p45) (CD38) Mouse anti-Human Monoclonal (HIT2) Antibody
101-M306 CD38 Anti-Human Host: Mouse CD38; T10 100
CSB-EL004929RA Rat CD38 molecule (CD38) ELISA kit, Species Rat, Sample Type serum, plasma 96T
A1680 CD38 Primary Antibody, CD38, Species: Human Recombinant Protein Source: Rabbit Polyclonal 50ug
20-783-70015 MOUSE ANTI HUMAN CD38 RPE - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 100 TESTS
20-783-70596 RAT ANTI MOUSE CD38 FITC - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 0.1 mg
20-783-70012 MOUSE ANTI HUMAN CD38 - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 0.2 mg
20-783-70016 MOUSE ANTI HUMAN CD38 - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 0.02 mg
20-783-70845 MOUSE ANTI HUMAN CD38 RPE-Cy5 - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 100 TESTS
20-783-70013 MOUSE ANTI HUMAN CD38 Biotin - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 0.1 mg
20-783-70014 MOUSE ANTI HUMAN CD38 FITC - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 0.1 mg
20-783-70598 RAT ANTI MOUSE CD38 - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 0.2 mg
20-783-70597 RAT ANTI MOUSE CD38 RPE - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 0.1 mg
Cd38-8742R Recombinant Rat Cd38, Fc-tagged species: Rat 20
Cd38-8743R Recombinant Rat Cd38, His-tagged species: Rat 20
20-783-70017 MOUSE ANTI HUMAN CD38 Azide Free - EC 3.2.2.5; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD38 antigen Monoclonal 1 mg
LF-MA41745 Anti-CD38 (6E12D), Mouse Monoclonal to CD38, Isotype IgG1, Host Mouse 50
LF-MA41726 Anti-CD38 (HIT2), Mouse Monoclonal to CD38, Isotype IgG1, Host Mouse 100
LF-MA41994 Anti-CD38 (HIT2), Mouse Monoclonal to CD38, Isotype IgG1, Host Mouse 50
LF-MA41297 anti-CD38(p45) (HIT2) , Mouse monoclonal to CD38(p45), Isotype IgG1, Host Mouse 50 ug
SCH-MCA1511PE RAT ANTI MOUSE CD38 RPE, Product Type Monoclonal Antibody, Specificity CD38, Target Species Mouse, Host Rat, Format RPE, Isotypes IgG2a, Applications F, Clone 90 0.1 mg
MCA1511PE RAT ANTI MOUSE CD38 RPE, Product Type Monoclonal Antibody, Specificity CD38, Target Species Mouse, Host Rat, Format RPE, Isotypes IgG2a, Applications F, Clone 90 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur