Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28921509
Publication Date : 2017/09/18

BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells.


Class-switching of B cells to IgA can be induced via both T-cell-dependent and T-cell-independent mechanisms. IgA is most predominantly produced mucosally and is important for combating infections and allergies. In contrast to mice, humans have two forms of IgA; IgA1 and IgA2 with diverse tissue distribution. In early life, IgA levels might be sub-optimal especially during the fall season when bacterial and viral infections are more common. Therefore, we investigated using human B cells whether T-cell-independent factors -promoting cell survival, class switching and immunoglobulin secretion- BAFF, APRIL, IL-10 and retinoic acid can boost IgA production in the context of viral or bacterial infection. To this end total and naive peripheral blood B cells were stimulated with these factors for 6 days in the presence or absence of TLR7/8 agonist R848 (mimicking viral infection) or TLR9 agonist CpG-ODN (mimicking bacterial infection). We show that BAFF significantly augments IgA2 production in TLR7/8 stimulated mature, but not naïve B cells. In addition, BAFF augments IL-10 production and viability in TLR7/8 and TLR9 stimulated mature B cells. These data warrant further investigation of its role in immune regulation both in the periphery and mucosal tissues in early life or during disease.

Authors : den Hartog Gerco , van Osch Thijs L J , Vos Martijn , Meijer Ben , Savelkoul Huub F J , van Neerven R J Joost , Brugman Sylvia ,

Related products :

Catalog number Product name Quantity
AK9587 G-CSF stimulated peripheral blood-derived mononuclear cells HUMAN CELLS Inquire
PBM001 Cryopreserved Human peripheral blood mononuclear cells (PBMC) HUMAN PERIPHERAL BLOOD CELLS 25 106 cells
PBL003-25M Cryopreserved Human peripheral blood lymphocytes (PBL) HUMAN PERIPHERAL BLOOD CELLS 25 106 cells
PBL003-10M Cryopreserved Human peripheral blood lymphocytes (PBL) HUMAN PERIPHERAL BLOOD CELLS 10 106 cells
SCH-LYS083A HUMAN PERIPHERAL BLOOD LEUKOCYTE TOTAL PROTEIN LYSATE, Product Type Cell Lysate, Specificity PERIPHERAL BLOOD LEUKOCYTE LYSATE, Target Species Human, Host N_A, Format Total Protein Lysate, Isotyp 0.5 mg
LYS083A HUMAN PERIPHERAL BLOOD LEUKOCYTE TOTAL PROTEIN LYSATE, Product Type Cell Lysate, Specificity PERIPHERAL BLOOD LEUKOCYTE LYSATE, Target Species Human, Host N_A, Format Total Protein Lysate, Isotyp 0.5 mg
LYS083 HUMAN PERIPHERAL BLOOD LEUKOCYTE TOTAL PROTEIN LYSATE, Product Type Cell Lysate, Specificity PERIPHERAL BLOOD LEUKOCYTE LYSATE, Target Species Human, Host N_A, Format Total Protein Lysate, Isotyp 0.1 mg
SCH-LYS083 HUMAN PERIPHERAL BLOOD LEUKOCYTE TOTAL PROTEIN LYSATE, Product Type Cell Lysate, Specificity PERIPHERAL BLOOD LEUKOCYTE LYSATE, Target Species Human, Host N_A, Format Total Protein Lysate, Isotyp 0.1 mg
LYS083B HUMAN PERIPHERAL BLOOD LEUKOCYTE TOTAL PROTEIN LYSATE, Product Type Cell Lysate, Specificity PERIPHERAL BLOOD LEUKOCYTE LYSATE, Target Species Human, Host N_A, Format Total Protein Lysate, Isotyp 1 mg
SCH-LYS083B HUMAN PERIPHERAL BLOOD LEUKOCYTE TOTAL PROTEIN LYSATE, Product Type Cell Lysate, Specificity PERIPHERAL BLOOD LEUKOCYTE LYSATE, Target Species Human, Host N_A, Format Total Protein Lysate, Isotyp 1 mg
AK9554 Peripheral blood mononuclear cells (PBMC) LRS chambers HUMAN CELLS Inquire
AK9559 Mobilized Peripheral Blood - CD133+ Stem_Progenitor Cells HUMAN CELLS Inquire
AK9553 Peripheral blood mononuclear cells (PBMC) HUMAN CELLS Inquire
DCM101 Cryopreserved Monocytes-derived dendritic cells HUMAN PERIPHERAL BLOOD CELLS 2 106 cells
ASE-5176 Peripheral Blood Mononuclear Cells (MS) 2.5x10^6 cells/vial
ASE-5163 Peripheral Blood Mononuclear Cells (ALL) 10x10^6 cells/vial
ASE-5177 Peripheral Blood Mononuclear Cells (MS) 25x10^6 cells/vial
ASE-5183 Peripheral Blood Mononuclear Cells (RA) 25x10^6 cells/vial
ASE-5174 Peripheral Blood Mononuclear Cells (MM) 25x10^6 cells/vial
ASE-5182 Peripheral Blood Mononuclear Cells (RA) 2.5x10^6 cells/vial
ASE-5171 Peripheral Blood Mononuclear Cells (CLL) 25x10^6 cells/vial
ASE-5167 Peripheral Blood Mononuclear Cells (AML) 2.5x10^6 cells/vial
ASE-5172 Peripheral Blood Mononuclear Cells (MM) 10x10^6 cells/vial
ASE-5164 Peripheral Blood Mononuclear Cells (ALL) 2.5 x 10^6 cells/vial
ASE-5179 Peripheral Blood Mononuclear Cells (NHL) 2.5 x 10^6 cells/vial


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur