Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :28928657
Publication Date : //

Colocynth Extracts Prevent Epithelial to Mesenchymal Transition and Stemness of Breast Cancer Cells.


Modern treatment strategies provide better overall survival in cancer patients, primarily by controlling tumor growth. However, off-target and systemic toxicity, tumor recurrence, and resistance to therapy are still inadvertent hurdles in current treatment regimens. Similarly, metastasis is another deadly threat to patients suffering from cancer. This has created an urgent demand to come up with new drugs having anti-metastatic potential and minimum side effects. Thus, this study was aimed at exploring the anti-proliferative and anti-metastatic potential of colocynth medicinal plant. Results from MTT assay, morphological visualization of cells and scratch assay indicated a role of ethanol and acetone extracts of fruit pulp of the colocynth plant in inhibiting cell viability, enhancing cell cytotoxicity and preventing cell migration in various cancer cell types, including breast cancer cell lines MCF-7 and MDA-MB-231, and cervical cancer cell line SiHa, subsequently having a low cytotoxic effect on mononuclear PBMC and macrophage J774A cells. Our study in metastatic MDA-MB-231 cells showed that both ethanol and acetone pulp extracts decreased transcript levels of the anti-apoptotic genes BCL2 and BCLXL, and a reverse effect was observed for the pro-apoptotic genes BAX and caspase 3. Additionally, enhanced caspase 3 activity and downregulated BCL2 protein were seen, indicating a role of these extracts in inducing apoptotic activity. Moreover, MDA-MB-231 cells treated with both these extracts demonstrated up-regulation of the epithelial gene keratin 19 and down-regulation of the mesenchymal genes, vimentin, N-cadherin, Zeb1 and Zeb2 compared to control, suggesting a suppressive impact of these extracts in epithelial to mesenchymal transition (EMT). In addition, these extracts inhibited colony and sphere formation with simultaneous reduction in the transcript level of the stemness associated genes, BMI-1 and CD44. It was also found that both the plant extracts exhibited synergistic potential with the chemotherapeutic drug doxorubicin to inhibit cancer viability. Furthermore, GC-MS/MS analysis revealed the presence of certain novel compounds in both the extracts that are responsible for the anti-cancer role of the extracts. Overall, the results of this report suggest, for the first time, that colocynth fruit pulp extracts may block the proliferative as well as metastatic activity of breast cancer cells.

Authors : Chowdhury Kaushik , Sharma Ankit , Kumar Suresh , Gunjan Gyanesh K , Nag Alo , Mandal Chandi C ,

Related products :

Catalog number Product name Quantity
EMT1H1_R4S Epithelial to Mesenchymal Transition (EMT) ,EMT1H1:4 SignArrays
EMT1H2_R4S Epithelial to Mesenchymal Transition (EMT) ,EMT1H2:4 SignArrays
EMT1H1_B12S Epithelial to Mesenchymal Transition (EMT),EMT1H1,12 SignArrays
EMT1H1_B24S Epithelial to Mesenchymal Transition (EMT),EMT1H1,24 SignArrays
EMT1H1_F8S Epithelial to Mesenchymal Transition (EMT),EMT1H1:8 SignArrays
EMT1H1_F2S Epithelial to Mesenchymal Transition (EMT),EMT1H1:2 SignArrays
EMT1H1_F24S Epithelial to Mesenchymal Transition (EMT),EMT1H1:24 SignArrays
EMT1H2_R2S Epithelial to Mesenchymal Transition (EMT) ,EMT1H2:2 SignArrays
EMT1H1_F4S Epithelial to Mesenchymal Transition (EMT),EMT1H1:4 SignArrays
EMT1H1_B8S Epithelial to Mesenchymal Transition (EMT),EMT1H1,8 SignArrays
EMT1H1_R8S Epithelial to Mesenchymal Transition (EMT) ,EMT1H1:8 SignArrays
EMT1H1_B2S Epithelial to Mesenchymal Transition (EMT),EMT1H1,2 SignArrays
EMT1H1_R2S Epithelial to Mesenchymal Transition (EMT) ,EMT1H1:2 SignArrays
EMT1H1_R12S Epithelial to Mesenchymal Transition (EMT) ,EMT1H1:12 SignArrays
EMT1H1_B4S Epithelial to Mesenchymal Transition (EMT),EMT1H1,4 SignArrays
EMT1H1_R24S Epithelial to Mesenchymal Transition (EMT) ,EMT1H1:24 SignArrays
EMT1H1_F12S Epithelial to Mesenchymal Transition (EMT),EMT1H1:12 SignArrays
4-20251 Epithelial Dissociation System 1 (Intestinal mesenchymal and epithelial,Liver epithelial,Mammary epithelial), Mouse and Rat ea
27-959 Fatty acid-binding protein 3 (FABP3) gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. 0.1 mg
Breast - BRC1502 Breast cancer array, non_overlapping with BRC1501, consists of duplicated 70 cases covering all the common types of breast cancer and 5 cases of normal and other non_malignant breast tissues. 1.1mm
Breast - BRC961 Breast cancer array consists of duplicated 36 cases covering all the common types of breast cancer and 12 cases of normal and other non_malignant breast tissues. 1.5mm
Breast - BRC1501 Breast cancer array consists of duplicated 70 cases covering all the common types of breast cancer and 5 cases of normal and other non_malignant breast tissues. 1.1mm
Breast - BRC481 Breast cancer array, 16 cases of breast cancer, each in duplicates, paired with corresponding uninvolved tissues from the same patients 2mm
27-912 Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and wat 0.1 mg
27-909 Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and wat 0.05 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur