Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29153978
Publication Date : //

The endometria of women with endometriosis exhibit dysfunctional expression of complement regulatory proteins during the mid secretory phase.


The control of complement activation within embryo-endometrium environment is critical for embryo survival. Cell evasion from complement attack requires interaction of complement regulatory proteins (CRPs) with cell adhesion αvβ3 integrin. We aim to compare the expression of CRPs in endometria of women with and without endometriosis and to examine the molecular interaction of decay accelerating factor (DAF) with αvβ3 integrin. Endometrial expression of Membrane cofactor protein (CD46), Decay accelerating factor (DAF), Membrane attack complex inhibitory factor (CD59) and β3 integrin subunit were determined through menstrual cycle by immunohistochemistry. DAF protein quantity was determined by Western blot and mRNA levels measured in epithelial cells isolated by laser capture microdissection (LCM). Using in vitro assay, we examined DAF and β3 integrin expression through paracrine regulation between endometrial compartments. To determine whether β3 integrin and DAF interacts in vivo, endometrial samples were subjected to immunoprecipitation and colocalization using dual immunofluorescence technique. DAF and β3 integrin expression were significantly low in samples from women with endometriosis during mid secretory phase. This observation was supported by decreased DAF protein quantity; faint DAF and β3 integrin interaction and reduced mRNA levels in cells dissected by LCM. Moreover epithelial DAF and β3 integrin expression through paracrine regulation by progesterone from stromal compartment was disrupted in endometriosis. Endometria from women with endometriosis exhibits aberrant expression of complement proteins. The abnormal DAF expression potentially compromises embryo survival, contributing to understand the implantation failure in women with endometriosis.

Authors : Palomino Wilder A , Tayade Chandrakant , Argandoña Felipe , Devoto Luigi , Young Steven L , Lessey Bruce A ,

Related products :

Catalog number Product name Quantity
32-208 Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50 percent similarity with Ras p21. Rab proteins play an important role fo 0.1 mg
30-298 The POU6F1 gene encodes a protein that is part of a family of transcription factors which exhibit distinct temporal and spatial patterns of expression. 0.05 mg
27-341 The POU6F1 gene encodes a protein that is part of a family of transcription factors which exhibit distinct temporal and spatial patterns of expression. 0.05 mg
26-241 The POU6F1 gene encodes a protein that is part of a family of transcription factors which exhibit distinct temporal and spatial patterns of expression. 0.05 mg
26-097 SKAP1 Is a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded prote 0.05 mg
29-671 CHGA is a member of the chromogranin_secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. Its gene_s product is a precursor to t 0.05 mg
29-670 CHGA is a member of the chromogranin_secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. Its gene_s product is a precursor to t 0.1 mg
25-704 PDZK1 is a scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. It may be involved in the coordina 0.05 mg
EGE-1400neo LEXSinduce2 Expression Kit contains vector pLEXSY_I_neo2 for inducible cytosolic or secretory expression 1 Kit
EGE-1410neo LEXSinduce3 Expression Kit contains vector pLEXSY_I_neo3 for inducible cytosolic or secretory expression 1 Kit
EGE-1300sat LEXSYcon2 Expression Kit contains vector pLEXSY_sat2 for constitutive cytosolic or secretory expression 1 Kit
EGE-1300neo LEXSYcon2 Expression Kit contains vector pLEXSY_neo2 for constitutive cytosolic or secretory expression 1 Kit
EGE-1410blecherry LEXSinduce3 Expression Kit contains vector pLEXSY_I_blecherry3 for inducible cytosolic or secretory expression 1 Kit
EGE-1300hyg LEXSYcon2 Expression Kit contains vector pLEXSY_hyg2 for constitutive cytosolic or secretory expression 1 Kit
EGE-1410ble LEXSinduce3 Expression Kit contains vector pLEXSY_I_ble3 for inducible cytosolic or secretory expression 1 Kit
EGE-1300ble LEXSYcon2 Expression Kit contains vector pLEXSY_ble2 for constitutive cytosolic or secretory expression 1 Kit
EGE-1400ble LEXSinduce2 Expression Kit contains vector pLEXSY_I_ble2 for inducible cytosolic or secretory expression 1 Kit
EGE-1300hyg LEXSYcon2 Expression Kit contains vector pLEXSY_hyg2 for constitutive cytosolic or secretory expression 1Kit
EGE-1410neo LEXSinduce3 Expression Kit contains vector pLEXSY_I_neo3 for inducible cytosolic or secretory expression 1 Kit
EGE-1300ble LEXSYcon2 Expression Kit contains vector pLEXSY_ble2 for constitutive cytosolic or secretory expression 1Kit
EGE-1300neo LEXSYcon2 Expression Kit contains vector pLEXSY_neo2 for constitutive cytosolic or secretory expression 1Kit
EGE-1300sat LEXSYcon2 Expression Kit contains vector pLEXSY_sat2 for constitutive cytosolic or secretory expression 1Kit
EGE-1400ble LEXSinduce2 Expression Kit contains vector pLEXSY_I_ble2 for inducible cytosolic or secretory expression 1Kit
EGE-1410ble LEXSinduce3 Expression Kit contains vector pLEXSY_I_ble3 for inducible cytosolic or secretory expression 1 Kit
EGE-1400neo LEXSinduce2 Expression Kit contains vector pLEXSY_I_neo2 for inducible cytosolic or secretory expression 1Kit


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur