Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29185745
Publication Date : //

Biosynthesis of Neokestose Laurate Catalyzed by Candida antarctica Lipase B and Its Antimicrobial Activity against Food Pathogenic and Spoilage Bacteria.


To increase the functionality and broaden the potential application of neokestose, neokestose laurate was biosynthesized using Candida antarctica lipase B as biocatalyst, for which a mixture of 20% DMSO in 2-methyl-2-butanol (v/v) was chosen as the reaction medium. The optimum conditions for biosynthesis were as follows: a molar ratio of vinyl laurate to neokestose of 12, a temperature of 50 °C, molecular sieves of 100 g/L, and enzyme loading of 10 g/L. Under the optimal conditions, the conversion rate was achieved over 80%. The synthesized chemical 6'-O-lauroylneokestose confirmed by nuclear magnetic resonance (NMR) exhibited good emulsification with critical micelle concentration (CMC) of 352 μM and broad antibacterial activity against Gram-positive bacteria such as Staphylococcus aureus, Listeria monocytogenes, Streptococcus mutans, Bacillus subtilis, and Bacillus cereus. Conclusively, 6'-O-lauroylneokestose was evidenced to be a dual-functional agent with emulsification and antibacterial activity, showing promising application potential in the food industry.

Authors : Ning Yawei , Chen Feng , Xu Xueming , Jin Yi , Wang Zhixin , Yang Kun , Jia Yingmin ,

Related products :

Catalog number Product name Quantity
TM 1425 CRYSTAL VIOLET TETRAZOLIUM AGAR BASE (for detection of gram_negative psychrotrophic bacteria causing food spoilage.) 500 gm
B477 Crystal Violet Tetrazolium Agar Base USE : For detection of gram negative psychrotrophic bacteria causing food spoilage. 5x500gm
B477 Crystal Violet Tetrazolium Agar Base USE For detection of gram negative psychrotrophic bacteria causing food spoilage. Qty per Litre of Medium: 23,5
B158 DNase Test Agar Base USE For detection of deoxyribonuclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci. Qty per Litre of Medium: 42
B158 DNase Test Agar Base USE : For detection of deoxyribonuclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci. 5x500gm
B580 D Nase Test Agar w_Methyl green USE For detection of deoxyribonuclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci. Qty per Litre of Medium: 42,05
B580 D Nase Test Agar w_Methyl green USE : For detection of deoxyribonuclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci. 5x500gm
TM 902 TRYPTOSE CYCLOSERINE DEXTROSE AGAR BASE (for isolation of mesophilic spore forming anaerobes in food spoilage.) 500 gm
B517 *Orange Serum Broth USE For cultivation of microorganisms associated with spoilage of citrus products. cultivation of Lactobacilli and other aciduric organisms and pathogenic fungi. Qty per Litre of Medium: 28,5
B1010 Dichloran Medium Base w_Rose Bengal USE For selective isolation and enumeration of yeasts and molds associated with food spoilage. Qty per Litre of Medium: 31,5
B1010 Dichloran Medium Base w_Rose Bengal USE : For selective isolation and enumeration of yeasts and molds associated with food spoilage. 5x500gm
B061 Tryptose Cycloserine Dextrose Agar Base USE : For the isolation of mesophilic spore forming anaerobes in food spoilage. 5x500gm
B061 Tryptose Cycloserine Dextrose Agar Base USE For the isolation of mesophilic spore forming anaerobes in food spoilage. Qty per Litre of Medium: 46
B519 *Orange Serum Agar USE For cultivation and enumeration of microorganisms associated with spoilage of citrus products.Cultivation of Lactobacilli and other aciduric organisms and pathogenic fungi. Qty per Litre of Medium: 45,5
B1274 Universal Liquid Medium, Modified USE : For cultivation of brewery bacteria, including beer spoilage forms in the brewing industry. 5x500gm
B1274 Universal Liquid Medium, Modified USE For cultivation of brewery bacteria, including beer spoilage forms in the brewing industry. Qty per Litre of Medium: 50
EDU 03 Detection of pathogenic bacteria (Salmonella) 100 reactions
EDU 03 Detection of pathogenic bacteria (Salmonella) 100
EDU 03 Detection of pathogenic bacteria (Salmonella) conventional PCR 100 REACTIONS
K017A Leptospira pathogenic CE, BACTERIA & PARASITES, conventional PCR 100 REACTIONS
EDU 03 Detection of pathogenic bacteria (Salmonella) , EDUCATION, conventional PCR 100 REACTIONS
TM 166 LIVER INFUSION AGAR (for cultivation of Brucella and other pathogenic bacteria) 500 gm
DLPS-100 QuantiChrom™ Lipase Assay Kit, Quantitative determination of lipase activity by colorimetric (412nm) method 100Tests
B227 Liver Infusion Agar USE : For cultivation of Brucella and other pathogenic bacteria. 5x500gm
B055 Veal Infusion Agar USE : For cultivation of fastidious pathogenic bacteria. 5x500gm


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur