Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29213065
Publication Date : //

Modulation Of Microtubule Acetylation By The Interplay Of TPPP/p25, SIRT2 And New Anticancer Agents With Anti-SIRT2 Potency.

The microtubule network exerts multifarious functions controlled by its decoration with various proteins and post-translational modifications. The disordered microtubule associated Tubulin Polymerization Promoting Protein (TPPP/p25) and the NAD-dependent tubulin deacetylase sirtuin-2 (SIRT2) play key roles in oligodendrocyte differentiation by acting as dominant factors in the organization of myelin proteome. Herein, we show that SIRT2 impedes the TPPP/p25-promoted microtubule assembly independently of NAD; however, the TPPP/p25-assembled tubulin ultrastructures were resistant against SIRT2 activity. TPPP/p25 counteracts the SIRT2-derived tubulin deacetylation producing enhanced microtubule acetylation. The inhibition of the SIRT2 deacetylase activity by TPPP/p25 is evolved by the assembly of these tubulin binding proteins into a ternary complex, the concentration-dependent formation of which was quantified by experimental-based mathematical modelling. Co-localization of the SIRT2-TPPP/p25 complex on the microtubule network was visualized in HeLa cells by immunofluorescence microscopy using Bimolecular Fluorescence Complementation. We also revealed that a new potent SIRT2 inhibitor (MZ242) and its proteolysis targeting chimera (SH1) acting together with TPPP/p25 provoke microtubule hyperacetylation, which is coupled with process elongation only in the case of the degrader SH1. Both the structural and the functional effects manifesting themselves by this deacetylase proteome could lead to the fine-tuning of the regulation of microtubule dynamics and stability.

Authors : Szabó Adél , Oláh Judit , Szunyogh Sándor , Lehotzky Attila , Szénási Tibor , Csaplár Marianna , Schiedel Matthias , Lőw Péter , Jung Manfred , Ovádi Judit ,

Related products :

Catalog number Product name Quantity
A0273 SIRT2 Polyclonal Antibody, Reactivity: H M R , Gene ID: 22933, Synonyms: SIRT2 100ug
A0273 SIRT2 Polyclonal Antibody, Reactivity: H M R , Gene ID: 22933, Synonyms: SIRT2 200ug
A0273 SIRT2 Primary Antibody, SIRT2, Species: Human Recombinant Protein Source: Rabbit Polyclonal 50ug
LF-MA10402 anti-SIRT2, Mouse monoclonal to SIRT2, Isotype IgG1 , Host Mouse 100 ug
DL-SIRT2-Hu Human Sirtuin 2 (SIRT2) ELISA Kit 96T
LF-PA42365 Anti-SIRT2, Rabbit Polyclonal to SIRT2, Isotype , Host Rabbit 50
LF-PA42364 Anti-SIRT2, Rabbit Polyclonal to SIRT2, Isotype , Host Rabbit 50
SIRT2 SIRT2 Gene sirtuin 2
MCA5067Z MOUSE ANTI HUMAN SIRT2 Azide free, Product Type Monoclonal Antibody, Specificity SIRT2, Target Species Human, Host Mouse, Format Azide Free, Isotypes IgG1, Applications IF, WB, Clone 4B11 0.1 mg
18-272-195659 SIR2 _ SIRT2 - Rabbit polyclonal to SIR2 _ SIRT2 Polyclonal 0.1 ml
orb93278 Mouse SIRT2 gene Mouse SIRT2 gene is a cDNA gene. For research use only. 10
orb91301 Human SIRT2 gene Human SIRT2 gene is a cDNA gene. For research use only. 10
YF-PA17679 anti-SIRT2 50 ul
LF-MA10402 anti-SIRT2(4B11) 100 ug
GWB-A5F529 Anti- SIR2 SIRT2 Antibody
GWB-A5F529 Anti- SIR2 _ SIRT2 Antibody
SIR2-201AP Rabbit Anti-SIRT2 Antibody 100
bs-7350R Rabbit Anti-SIRT2 Polyclonal Antibody 100ul
bs-7350R-Cy7 Rabbit Anti-SIRT2 Polyclonal Antibody, Cy7 Conjugated 100ul
bs-7350R-Cy5.5 Rabbit Anti-SIRT2 Polyclonal Antibody, Cy5.5 Conjugated 100ul
LS-B1565-50 SIRT2 Rabbit anti-Human Polyclonal Antibody
bs-7350R-Cy5 Rabbit Anti-SIRT2 Polyclonal Antibody, Cy5 Conjugated 100ul
bs-7350R-Cy3 Rabbit Anti-SIRT2 Polyclonal Antibody, Cy3 Conjugated 100ul
ARP32385_P100 Anti-NAD-dependent deacetylase sirtuin-2 (SIRT2) Species_Reactivity: Human


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017


france@gentaur.com | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur