GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Potential role of salt-bridges in the hinge-like movement of apicomplexa specific β-hairpin of Plasmodium and Toxoplasma profilins: A molecular dynamics simulation study.

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29236299
Publication Date : //

Potential role of salt-bridges in the hinge-like movement of apicomplexa specific β-hairpin of Plasmodium and Toxoplasma profilins: A molecular dynamics simulation study.

Profilin is one of the actin-binding proteins that regulate dynamics of actin polymerization. It plays a key role in cell motility and invasion. It also interacts with several other proteins notably through its poly-L-proline (PLP) binding site. Profilin in apicomplexa is characterized by a unique mini-domain consisting of a large β-hairpin extension and an acidic loop which is relatively longer in Plasmodium species. Profilin is essential for the invasive blood stages of Plasmodium falciparum. In the current study, unbound profilins from Plasmodium falciparum (Pf), Toxoplasma gondii (Tg), and Homo sapiens (Hs) were subjected to molecular dynamics (MD) simulations for a timeframe of 100 ns each to understand the conformational dynamics of these proteins. It was found that the β-hairpin of profilins from Pf and Tg shows a hinge-like movement. This movement in Pf profilin may possibly be driven by the loss of a salt-bridge within profilin. The impact of this conformational change on actin binding was assessed by docking three dimensional (3D) structures of profilin from Pf and Tg with their corresponding actins using ClusPro2.0. The stability of docked Pf profilin-actin complex was assessed through a 50 ns MD simulation. As Hs profilin I does not have the apicomplexa specific mini-domain, MD simulation was performed for this protein and its dynamics was compared to that of profilins from Pf and Tg. Using an immunoinformatics approach, potential epitope regions were predicted for Pf profilin. This has a potential application in the design of vaccines as they mapped to its unique mini-domain.

Authors : Kadirvel Priyadarsini , Anishetty Sharmila ,

Related products :

Catalog number	Product name	Quantity

DEIA379	Toxoplasma;Toxoplasma gondii; Eukaryota; Chromalveolata; Alveolata; Apicomplexa; Conoidasida; Coccidiasina; Eucoccidiorida; Sarcocystidae;T. gondii	96T
DEIA379	Toxoplasma;Toxoplasma gondii; Eukaryota; Chromalveolata; Alveolata; Apicomplexa; Conoidasida; Coccidiasina; Eucoccidiorida; Sarcocystidae;T. gondii	96T
DEIA379	Toxoplasma;Toxoplasma gondii; Eukaryota; Chromalveolata; Alveolata; Apicomplexa; Conoidasida; Coccidiasina; Eucoccidiorida; Sarcocystidae;T. gondii	96T
DEIA380	Toxoplasma;Toxoplasma gondii; Eukaryota; Chromalveolata; Alveolata; Apicomplexa; Conoidasida; Coccidiasina; Eucoccidiorida; Sarcocystidae;T. gondii	96T
DEIA380	Toxoplasma;Toxoplasma gondii; Eukaryota; Chromalveolata; Alveolata; Apicomplexa; Conoidasida; Coccidiasina; Eucoccidiorida; Sarcocystidae;T. gondii	96T
DEIA380	Toxoplasma;Toxoplasma gondii; Eukaryota; Chromalveolata; Alveolata; Apicomplexa; Conoidasida; Coccidiasina; Eucoccidiorida; Sarcocystidae;T. gondii	96T
27-175	KPTN may be involved in actin dynamics. KPTN may play a role in producing the sensory apparatus in hair cells. KPTN may also play a role in actin rearrangements that accompany platelet activation and	0.05 mg
2426	MOLECULAR ANEUPLOIDY STUDY, AMNIOTIC FLUID	1
8478	MOLECULAR ANEUPLOIDY STUDY, CHORIAL VILLOSITY	1
31-317	LRRTM1 may play a role during the development of specific forebrain structures by influencing neuronal differentiation and connectivity, with a possible role in intracellular trafficking within axons.	0.05 mg
30-255	MTCH1 is a potential mitochondrial transporter. It may play a role in apoptosis.	0.05 mg
30-247	The LETM1 is a factor of the mitochondrial K+ homeostasis with a potential role in the Wolf-Hirschhorn syndrome.	0.1 mg
28-992	GMEB2 is a member of KDWK family. GMEB2 associates with GMEB1 protein, and the complex is essential for parvovirus DNA replication. Study of rat homolog implicates the role of this gene in modulation	0.1 mg
30-711	In vitro study revealed that RSRC2 might play a role in cell proliferation. RSRC2 may be a novel tumor suppressor of esophageal cancer cell growth.	0.1 mg
K405	Plasmodium multiplex+ Plasmodium vivax & Plasmodium falciparum , BLOOD PARASITES, conventional PCR	100 REACTIONS
MD80001	MagCustom Feasibility Study, Bead development on client demand in small scale as feasability study	1 item
30-798	NudC was first identified as a regulator of nuclear movement in the asexual reproductive cycle of the filamentous fungus Aspergillus nidulans. Human NUDC is a nuclear movement protein that associates	0.05 mg
K405	Plasmodium multiplex+ Plasmodium vivax & Plasmodium falciparum	100 reactions
K405	Plasmodium multiplex+ Plasmodium vivax & Plasmodium falciparum	100 REACTIONS
K405	Plasmodium multiplex+ Plasmodium vivax & Plasmodium falciparum	100
26-484	Potassium channels play a role in many cellular processes including maintenance of the action potential, muscle contraction, hormone secretion, osmotic regulation, and ion flow. KCNK4 is one of the me	0.05 mg
30-583	Potassium channels play a role in many cellular processes including maintenance of the action potential, muscle contraction, hormone secretion, osmotic regulation, and ion flow. KCNK4 is one of the me	0.05 mg
AM08151AP-N	Human IgG1 (hinge) (gamma_1 chain specific)	1.0 ml
AM08151RP-N	Human IgG1 (hinge) (gamma_1 chain specific)	0.1 mg
AM08151PU-N	Human IgG1 (hinge) (gamma_1 chain specific)	0.5 mg

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel: 0208-080893 Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62 SWIFT RABONL2U

GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur

ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF