Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29269218
Publication Date : //

Temporal and SUMO-specific SUMOylation contribute to the dynamics of Polo-like kinase 1 (PLK1) and spindle integrity during mouse oocyte meiosis.


During mammalian meiosis, Polo-like kinase 1 (PLK1) is essential during cell cycle progression. In oocyte maturation, PLK1 expression is well characterized but timing of posttranslational modifications regulating its activity and subcellular localization are less clear. Small ubiquitin-related modifier (SUMO) posttranslational modifier proteins have been detected in mammalian gametes but their precise function during gametogenesis is largely unknown. In the present paper we report for mouse oocytes that both PLK1 and phosphorylated PLK1 undergo SUMOylation in meiosis II (MII) oocytes using immunocytochemistry, immunoprecipitation and in vitro SUMOylation assays. At MII, PLK1 is phosphorylated at threonine-210 and serine-137. MII oocyte PLK1 and phosphorylated PLK1 undergo SUMOylation by SUMO-1, -2 and -3 as shown by individual in vitro assays. Using these assays, forms of phosphorylated PLK1 normalized to PLK1 increased significantly and correlated with SUMOylated PLK1 levels. During meiotic progression and maturation, SUMO-1-SUMOylation of PLK1 is involved in spindle formation whereas SUMO-2/3-SUMOylation may regulate PLK1 activity at kinetochore-spindle attachment sites. Microtubule integrity is required for PLK1 localization with SUMO-1 but not with SUMO-2/3. Inhibition of SUMOylation disrupts proper meiotic bipolar spindle organization and spindle-kinetochore attachment. The data show that both temporal and SUMO-specific-SUMOylation play important roles in orchestrating functional dynamics of PLK1 during mouse oocyte meiosis, including subcellular compartmentalization.

Authors : Feitosa Weber Beringui , Hwang KeumSil , Morris Patricia L ,

Related products :

Catalog number Product name Quantity
E1662r ELISA Plk,Plk1,PLK-1,Polo-like kinase 1,Rat,Rattus norvegicus,Serine_threonine-protein kinase PLK1 96T
E1662b ELISA Bos taurus,Bovine,PLK1,PLK-1,Polo-like kinase 1,Serine_threonine-protein kinase PLK1 96T
E1662b ELISA kit Bos taurus,Bovine,PLK1,PLK-1,Polo-like kinase 1,Serine_threonine-protein kinase PLK1 96T
U1662r CLIA Plk,Plk1,PLK-1,Polo-like kinase 1,Rat,Rattus norvegicus,Serine_threonine-protein kinase PLK1 96T
E1662r ELISA kit Plk,Plk1,PLK-1,Polo-like kinase 1,Rat,Rattus norvegicus,Serine_threonine-protein kinase PLK1 96T
U1662b CLIA Bos taurus,Bovine,PLK1,PLK-1,Polo-like kinase 1,Serine_threonine-protein kinase PLK1 96T
E1662m ELISA kit Mouse,Mus musculus,Plk,Plk1,PLK-1,Polo-like kinase 1,Serine_threonine-protein kinase 13,Serine_threonine-protein kinase PLK1,STPK13 96T
U1662m CLIA Mouse,Mus musculus,Plk,Plk1,PLK-1,Polo-like kinase 1,Serine_threonine-protein kinase 13,Serine_threonine-protein kinase PLK1,STPK13 96T
E1662m ELISA Mouse,Mus musculus,Plk,Plk1,PLK-1,Polo-like kinase 1,Serine_threonine-protein kinase 13,Serine_threonine-protein kinase PLK1,STPK13 96T
E1662h ELISA kit Homo sapiens,Human,PLK,PLK1,PLK-1,Polo-like kinase 1,Serine_threonine-protein kinase 13,Serine_threonine-protein kinase PLK1,STPK13 96T
E1662h ELISA Homo sapiens,Human,PLK,PLK1,PLK-1,Polo-like kinase 1,Serine_threonine-protein kinase 13,Serine_threonine-protein kinase PLK1,STPK13 96T
U1662h CLIA Homo sapiens,Human,PLK,PLK1,PLK-1,Polo-like kinase 1,Serine_threonine-protein kinase 13,Serine_threonine-protein kinase PLK1,STPK13 96T
UB-E21216 Mouse Polo-like kinase 1(PLK1)ELISA Kit 96T
QY-E21216 Mouse Polo-like kinase 1(PLK1)ELISA Kit 96T
UB-E21179 Mouse Phospho-Polo-like kinase 1(P-PLK1)ELISA Kit 96T
QY-E21179 Mouse Phospho-Polo-like kinase 1(P-PLK1)ELISA Kit 96T
GWB-FC20DD Polo-Like Kinase (PLK) (PLK1) Mouse anti-Human Monoclonal (pThr210) (2A3) Antibody
CSB-EL018193MO Mouse polo-like kinase 1 (Drosophila) (PLK1) ELISA kit, Species Mouse, Sample Type serum, plasma 96T
QY-E10527 Rat Polo-like kinase 1(PLK1)ELISA Kit 96T
201-11-1118 Rat Polo-like kinase 1(PLK1)ELISA Kit 96T
PLK2 PLK1 Gene polo-like kinase 1
UB-E10527 Rat Polo-like kinase 1(PLK1)ELISA Kit 96T
201-11-1118 Rat Polo-like kinase 1,PLK1 ELISA Kit 96T
E2202Hu Human Polo-like kinase 1,PLK1 ELISA Kit 48T
201-12-2203 Human Polo-like kinase 1,PLK1 ELISA Kit 48T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur