Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29355585
Publication Date : //

High degree of correlation between Ebola virus BSL-4 neutralization assays and pseudotyped VSV BSL-2 fluorescence reduction neutralization test.


Ebola virus (EBOV), classified as a category A agent by the CDC and NIH, requires BSL-4 containment and induces high morbidity and mortality in humans. The 2013-2015 epidemic in West Africa underscored the urgent need to develop vaccines and therapeutics to prevent and treat EBOV disease. Neutralization assays are needed to evaluate the efficacy of EBOV vaccines and antibody therapies. Pseudotyped viruses based on nonpathogenic or attenuated vectors reduce the risks involved in the evaluation of neutralizing antibodies against highly pathogenic viruses. Selectable markers, fluorescent proteins, and luciferase have been introduced into pseudotyped viruses for detection and quantitation purposes. The current study describes the development of a BSL-2 fluorescence reduction neutralization test (FRNT) using a recombinant vesicular stomatitis virus (VSV) in which the VSV-G envelope gene was replaced with the EBOV glycoprotein (GP) and green fluorescent protein (GFP) genes (rVSV-EBOVgp-GFP). Cells infected with rVSV-EBOVgp-GFP express GFP. Anti-GP neutralizing monoclonal and polyclonal antibodies blocked rVSV-EBOVgp-GFP infection preventing or reducing GFP fluorescence. The high degree of correlation between the EBOV BSL-2 FRNT and the BSL-4 plaque reduction neutralization test (PRNT), the accepted standard of EBOV neutralization tests, supports the use of the EBOV BSL-2 FRNT to evaluate neutralizing antibodies in clinical trials.

Authors : Konduru Krishnamurthy , Shurtleff Amy C , Bavari Sina , Kaplan Gerardo ,

Related products :

Catalog number Product name Quantity
YCC310-808 Staphylococcus aureus, Coagulase, type VII, Rabbit anti_, for neutralization test 5 ml.
YCC310-802 Staphylococcus aureus, Coagulase, type I, Rabbit anti_, for neutralization test 5 ml.
YCC310-803 Staphylococcus aureus, Coagulase, type II, Rabbit anti_, for neutralization test 5 ml.
YCC310-804 Staphylococcus aureus, Coagulase, type III, Rabbit anti_, for neutralization test 5 ml.
YCC310-805 Staphylococcus aureus, Coagulase, type IV, Rabbit anti_, for neutralization test 5 ml.
YCC310-806 Staphylococcus aureus, Coagulase, type V, Rabbit anti_, for neutralization test 5 ml.
YCC310-807 Staphylococcus aureus, Coagulase, type VI, Rabbit anti_, for neutralization test 5 ml.
YCC310-809 Staphylococcus aureus, Coagulase, type VIII, Rabbit anti_, for neutralization test 5 ml.
YCC320-102 Staphylococcus aureus, Coagulase Typing Reagent,Normal Rb.Serum (does not contain citrate), for neutralization test 3 ml.
YCC320-103 Staphylococcus aureus, Coagulase Typing Reagent,Normal Rabbit Plasma (contains citrate), for neutralization test 3 ml.
YCC320-101 Staphylococcus aureus, Coagulase Typing Reagent, Diluent (Polypeptone, Na citrate, NaN3), for neutralization test 20 ml.
YCC310-801 Staphylococcus aureus, Coagulase, Typing (includes types I_VIII), Rabbit anti_, Set, for neutralization test set
EC-876-1L NEUTRALIZATION SOLUTION 1L
EC-876-1L NEUTRALIZATION SOLUTION LITRE
EC-876-1L NEUTRALIZATION SOLUTION LITRE
QP0543 QGP DNA Neutralization Buffer 23 mL
BN004 Neutralization buffer II 500ml
BN005 Neutralization buffer III 5x500ml
BN004 Neutralization buffer II 5x500ml
BN003 Neutralization buffer I 5x1000ml
BN003 Neutralization buffer I 1000ml
BN001 Neutralization buffer, pH 7.4 500ml
55R-1003 Antithrombin III Neutralization kit 1 kit
BN001 Neutralization buffer, pH 7.4 5x500ml
55R-1004 HCII Neutralization kit 1 kit


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur