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From laboratory to point of entry: development and implementation of a loop-mediated isothermal amplification (LAMP)-based genetic identification system to prevent introduction of quarantine insect species.
Rapid genetic on-site identification methods at points of entry, such as seaports and airports, have the potential to become important tools to prevent the introduction and spread of economically harmful pest species that are unintentionally transported by the global trade of plant commodities. This paper reports the development and evaluation of a loop-mediated isothermal amplification (LAMP)-based identification system to prevent introduction of the three most frequently encountered regulated quarantine insect species groups at Swiss borders, Bemisia tabaci, Thrips palmi and several regulated fruit flies of the genera Bactrocera and Zeugodacus.
Authors : Blaser Simon , Diem Hanspeter , von Felten Andreas , Gueuning Morgan , Andreou Michael , Boonham Neil , Tomlinson Jennifer , Müller Pie , Utzinger Jürg , Frey Jürg E , Bühlmann Andreas ,
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Pathways :
WP1069: Integrin-mediated cell adhesion
WP1185: Integrin-mediated cell adhesion
WP1386: Integrin-mediated cell adhesion
WP1453: Vulval development
WP1502: Mitochondrial biogenesis
WP1537: E2F/MIRHG1 feedback-loop
WP1591: Heart Development
WP1624: Bacterial secretion system
WP1713: Two-component system
WP1780: ABC-family proteins mediated transport
WP1782: APC/C-mediated degradation of cell cycle proteins
WP1815: Factors involved in megakaryocyte development and platelet production
WP1826: GPVI-mediated activation cascade
WP1845: MAPK targets/ Nuclear events mediated by MAP kinases
WP185: Integrin-mediated cell adhesion
WP1897: Regulation of beta-cell development
WP1904: RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
WP1908: Signal amplification
WP1922: SLC-mediated transmembrane transport
WP1968: genetic alternations of lung cancer
WP2067: Heart Development
WP2108: flower development (initiation)
WP2199: Seed Development
WP2220: Notch signaling in Vulval Development
WP2221: EGF Signaling in Vulval Development
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[29363271] From laboratory to point of entry: development and implementation of a loop-mediated isothermal amplification (LAMP)-based genetic identification system to prevent introduction of quarantine insect species.
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