Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29556340
Publication Date : //

The role of PTRF/Cavin1 as a biomarker in both glioma and serum exosomes.


Exosomes play critical roles in intercellular communication in both nearby and distant cells in individuals and organs. Polymerase I and transcript release factor (PTRF), also known as Cavin1, has previously been described as a critical factor in caveola formation, and aberrant PTRF expression has been reported in various malignancies. However, the function of PTRF in tumor progression remains controversial, and its role in glioma is poorly understood. In this study, we report that PTRF is associated with malignancy grade and poor prognosis in glioma patients. Our previous study using two proteomics methods, tandem mass tag (TMT) and data-independent acquisition (DIA), showed that EGFRvIII overexpression increased PTRF expression at the protein level. In contrast, blocking PI3K and AKT using LY294002 and MK-2206, respectively, decreased PTRF expression, showing that PTRF is regulated in the EGFR/PI3K/AKT pathway. ChIP-PCR analysis showed that PTRF is transcriptionally regulated by the H3K4me3 and H3K27me3 modifications. Furthermore, PTRF overexpression increased exosome secretion and induced cell growth in vitro. More importantly, overexpressing PTRF induced the malignancy of nearby cells in vivo, suggesting that PTRF alters the microenvironment through intercellular communication via exosomes. Furthermore, analysis of clinical samples showed a positive correlation between tumor grade and PTRF expression in both tumor tissues and exosomes isolated from blood harvested from glioma patients, and PTRF expression in exosomes isolated from the sera of GBM patients was decreased after surgery. In conclusion, PTRF serves as a promising biomarker in both tumor samples and serum exosomes, thus facilitating the detection of glioma and potentially serving as a therapeutic target for glioblastoma multiforme.

Authors : Huang Kai , Fang Chuan , Yi Kaikai , Liu Xing , Qi Hongzhao , Tan Yanli , Zhou Junhu , Li Ying , Liu Mingyang , Zhang Yuqing , Yang Jingxuan , Zhang Jianning , Li Min , Kang Chunsheng ,

Related products :

Catalog number Product name Quantity
PTRF PTRF Gene polymerase I and transcript release factor
NGL481 Nervous system glioma tissue array consists of duplicated 24 cases of normal brain and glioma tissues. Coming soon. 2mm
27-076 TSPAN33 plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors. 0.05 mg
CSB-EL019070RA Rat polymerase I and transcript release factor (PTRF) ELISA kit, Species Rat, Sample Type serum, plasma 96T
CSB-EL012898RA Rat leucine-rich, glioma inactivated 1 (LGI1) ELISA kit, Species Rat, Sample Type serum, plasma 96T
31-317 LRRTM1 may play a role during the development of specific forebrain structures by influencing neuronal differentiation and connectivity, with a possible role in intracellular trafficking within axons. 0.05 mg
31-144 The SERPINF1 gene may play a significant role in determining the balance of angiogenesis_ antiangiogenesis during atherogenesis. A novel role of extracellular phosphorylation is shown to completely ch 0.05 mg
30-347 The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. STRAP may play a role in the cellular distribution of the 0.05 mg
30-348 The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. STRAP may play a role in the cellular distribution of the 0.1 mg
MCA4601Z MOUSE ANTI HUMAN PTRF Azide Free, Product Type Monoclonal Antibody, Specificity PTRF, Target Species Human, Host Mouse, Format Azide Free, Isotypes IgG2b, Applications P*, Clone 1F7 0.1 mg
27-255 Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is requir 0.05 mg
CSB-EL019070MO Mouse polymerase I and transcript release factor (PTRF) ELISA kit, Species Mouse, Sample Type serum, plasma 96T
CSB-EL019070HU Human polymerase I and transcript release factor (PTRF) ELISA kit, Species Human, Sample Type serum, plasma 96T
V01002402-096 Salmonid Vtg Biomarker ELISA kit 96 wells
V01002402-480 Salmonid Vtg Biomarker ELISA kit 480 wells
26-525 DBX1 could have a role in patterning the central nervous system during embryogenesis. DBX1 has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral i 0.05 mg
26-150 DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta_serrate_jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell- 0.05 mg
OC-K-002 Obesity Biomarker Chip Kit (Human) _ 2 Slide Kit 1Kit
B00400404-480 Biomarker ELISA component Kit PAb GAR_HRP 480 wells
B00400402-096 Biomarker ELISA component Kit MAb GAM_HRP 96 wells
B00400402-480 Biomarker ELISA component Kit MAb GAM_HRP 480 wells
OC-K-003 Obesity Biomarker Chip Kit (Rat, Mouse) _ 2 Slide Kit 1Kit
B00400404-096 Biomarker ELISA component Kit PAb GAR_HRP 96 wells
CSB-EL012898HU Human leucine-rich, glioma inactivated 1 (LGI1) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL012898BO Bovine leucine-rich, glioma inactivated 1 (LGI1) ELISA kit, Species Bovine, Sample Type serum, plasma 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur