Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29565245
Publication Date : //

Human LINE-1 retrotransposition requires a metastable coiled coil and a positively charged N-terminus in L1ORF1p.


LINE-1 (L1) is an autonomous retrotransposon, which acted throughout mammalian evolution and keeps contributing to human genotypic diversity, genetic disease and cancer. L1 encodes two essential proteins: L1ORF1p, a unique RNA-binding protein, and L1ORF2p, an endonuclease and reverse transcriptase. L1ORF1p contains an essential, but rapidly evolving N-terminal portion, homo-trimerizes via a coiled coil and packages L1RNA into large assemblies. Here, we determined crystal structures of the entire coiled coil domain of human L1ORF1p. We show that retrotransposition requires a non-ideal and metastable coiled coil structure, and a strongly basic L1ORF1p amino terminus. Human L1ORF1p therefore emerges as a highly calibrated molecular machine, sensitive to mutation but functional in different hosts. Our analysis rationalizes the locally rapid L1ORF1p sequence evolution and reveals striking mechanistic parallels to coiled coil-containing membrane fusion proteins. It also suggests how trimeric L1ORF1p could form larger meshworks and indicates critical novel steps in L1 retrotransposition.

Authors : Khazina Elena , Weichenrieder Oliver ,

Related products :

Catalog number Product name Quantity
EIAAB07128 C9orf49,CHCHD2P9,CHCHD9,Coiled-coil-helix-coiled-coil-helix domain-containing 2 pseudogene 9,Homo sapiens,Human,Putative coiled-coil-helix-coiled-coil-helix domain-containing protein CHCHD2P9, mitocho
CSB-EL005324HU Human Putative coiled-coil-helix-coiled-coil-helix domain-containing protein CHCHD2P9, mitochondrial(CHCHD9) ELISA kit SpeciesHuman 96T
CSB-EL005317HU Human coiled-coil-helix-coiled-coil-helix domain containing 2 (CHCHD2) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL005322HU Human coiled-coil-helix-coiled-coil-helix domain containing 7 (CHCHD7) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL005320HU Human coiled-coil-helix-coiled-coil-helix domain containing 5 (CHCHD5) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL005324HU Human coiled-coil-helix-coiled-coil-helix domain containing 9 (CHCHD9) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL005319HU Human coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL005318HU Human coiled-coil-helix-coiled-coil-helix domain containing 3 (CHCHD3) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL005321HU Human coiled-coil-helix-coiled-coil-helix domain containing 6 (CHCHD6) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL005315HU Human coiled-coil-helix-coiled-coil-helix domain containing 1 (CHCHD1) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL005316HU Human coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) ELISA kit, Species Human, Sample Type serum, plasma 96T
CSB-EL005323HU Human coiled-coil-helix-coiled-coil-helix domain containing 8 (CHCHD8) ELISA kit, Species Human, Sample Type serum, plasma 96T
CHCH3_HUMAN ELISA Kit FOR Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial; organism: Human; gene name: CHCHD3 96T
CHCH2_HUMAN ELISA Kit FOR Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial; organism: Human; gene name: CHCHD2 96T
CHC10_HUMAN ELISA Kit FOR Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial; organism: Human; gene name: CHCHD10 96T
G5255 Putative coiled-coil-helix-coiled-coil-helix domain-containing protein CHCHD2P9, mitochondrial (CHCHD9), Human, ELISA Kit 96T
CSB-EL005324HU Human Putative coiled-coil-helix-coiled-coil-helix domain-containing protein CHCHD2P9, mitochondrial(CHCHD9) ELISA kit 96T
CHCH8_HUMAN ELISA Kit FOR Coiled-coil-helix-coiled-coil-helix domain-containing protein 8; organism: Human; gene name: CHCHD8 96T
CSB-EL005316HU Human Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial(CHCHD10) ELISA kit SpeciesHuman 96T
EIAAB07117 CHCHD3,Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial,Homo sapiens,Human
EIAAB07120 C2orf9,CHCHD5,Coiled-coil-helix-coiled-coil-helix domain-containing protein 5,Homo sapiens,Human
CSB-EL005317HU Human Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial(CHCHD2) ELISA kit SpeciesHuman 96T
CHCH7_HUMAN ELISA Kit FOR Coiled-coil-helix-coiled-coil-helix domain-containing protein 7; organism: Human; gene name: CHCHD7 96T
CSB-EL005318HU Human Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial(CHCHD3) ELISA kit SpeciesHuman 96T
CHCH6_HUMAN ELISA Kit FOR Coiled-coil-helix-coiled-coil-helix domain-containing protein 6; organism: Human; gene name: CHCHD6 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur