Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29648658
Publication Date : //

Pseudomonas aeruginosa partitioning protein ParB acts as a nucleoid-associated protein binding to multiple copies of a parS-related motif.


ParA and ParB homologs are involved in accurate chromosome segregation in bacteria. ParBs participate in the separation of ori domains by binding to parS palindromes, mainly localized close to oriC. In Pseudomonas aeruginosa neither ParB deficiency nor modification of all 10 parSs is lethal. However, such mutants show not only defects in chromosome segregation but also growth retardation and motility dysfunctions. Moreover, a lack of parB alters expression of over 1000 genes, suggesting that ParB could interact with the chromosome outside its canonical parS targets. Here, we show that indeed ParB binds specifically to hundreds of sites in the genome. ChIP-seq analysis revealed 420 ParB-associated regions in wild-type strain and around 1000 in a ParB-overproducing strain and in various parS mutants. The vast majority of the ParB-enriched loci contained a heptanucleotide motif corresponding to one arm of the parS palindrome. All previously postulated parSs, except parS5, interacted with ParB in vivo. Whereas the ParB binding to the four parS sites closest to oriC, parS1-4, is involved in chromosome segregation, its genome-wide interactions with hundreds of parS half-sites could affect chromosome topology, compaction and gene expression, thus allowing P. aeruginosa ParB to be classified as a nucleoid-associated protein.

Authors : Kawalek Adam , Bartosik Aneta A , Glabski Krzysztof , Jagura-Burdzy Grazyna ,

Related products :

Catalog number Product name Quantity
IMS01-133-332 Antibody: Pseudomonas aeruginosa , Host: hen, polyclonal, Comfirmed reactivity: Pseudomonas aeruginosa strains PAO1 and Habs1 200
EIAAB34073 Homo sapiens,hRBMY,Human,RBM1,RBM2,RBMY1A1,RNA-binding motif protein 1,RNA-binding motif protein 2,RNA-binding motif protein, Y chromosome, family 1 member A1_C,Y chromosome RNA recognition motif 1,YR
OBT7049 RAT ANTI PSEUDOMONAS AERUGINOSA, Product Type Polyclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Rat, Format Serum, Isotypes Polyclonal IgG, Applications E, 0.1 ml
SCH-OBT7049 RAT ANTI PSEUDOMONAS AERUGINOSA, Product Type Polyclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Rat, Format Serum, Isotypes Polyclonal IgG, Applications E, 0.1 ml
MCA4757 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPES 7 & 8, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG2a, Ap 0.2 mg
SCH-MCA4757 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPES 7 & 8, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG2a, Ap 0.2 mg
7889-9007 MOUSE ANTI PSEUDOMONAS AERUGINOSA, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG2a, Applications E, C 0.2 mg
SCH-7889-9007 MOUSE ANTI PSEUDOMONAS AERUGINOSA, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG2a, Applications E, C 0.2 mg
MCA4765 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPE 6, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG2a, Applica 0.2 mg
SCH-MCA4765 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPE 6, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG2a, Applica 0.2 mg
EIAAB34014 Rat,Rattus norvegicus,Rbm30,Rbm4b,RNA-binding motif protein 30,RNA-binding motif protein 4B,RNA-binding protein 30,RNA-binding protein 4B,Zinc-responsive protein ZD7
SCH-7889-9108 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPE 3, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applicat 0.2 mg
7889-9099 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPE 2B, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applica 0.2 mg
7889-9108 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPE 3, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applicat 0.2 mg
7889-9198 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPE 9, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG1, Applicat 0.2 mg
SCH-7889-9198 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPE 9, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG1, Applicat 0.2 mg
SCH-7889-9099 MOUSE ANTI PSEUDOMONAS AERUGINOSA SEROTYPE 2B, Product Type Monoclonal Antibody, Specificity PSEUDOMONAS AERUGINOSA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applica 0.2 mg
28-117 LARP2 encodes a protein containing domains found in the La related protein of Drosophila melanogaster. La motif-containing proteins are thought to be RNA-binding proteins, where the La motif and adjac 0.1 mg
EIAAB34015 Homo sapiens,Human,RBM30,RBM4B,RNA-binding motif protein 30,RNA-binding motif protein 4B,RNA-binding protein 30,RNA-binding protein 4B
EIAAB33986 hLark,Homo sapiens,Human,Lark homolog,RBM4,RBM4A,RNA-binding motif protein 4,RNA-binding motif protein 4a,RNA-binding protein 4
EIAAB33984 Lark homolog,mLark,Mouse,Mus musculus,Rbm4,Rbm4a,RNA-binding motif protein 4,RNA-binding motif protein 4a,RNA-binding protein 4
EIAAB34012 Mouse,Mus musculus,Rbm30,Rbm4b,RNA-binding motif protein 30,RNA-binding motif protein 4B,RNA-binding protein 30,RNA-binding protein 4B
20-372-60051 RNA binding motif protein 9 (RBM9) - Mouse monoclonal anti-human RBM9 antibody; RNA-binding motif protein 9; Hexaribonucleotide-binding protein 2; Repressor of tamoxifen transcriptional activity Monoc 0.1 mg
EIAAB33964 Arg_Glu_Asp-rich protein of 120 kDa,Homo sapiens,Human,Protein S164,RBM25,RED120,RNA-binding motif protein 25,RNA-binding protein 25,RNA-binding region-containing protein 7,RNPC7
EIAAB12809 ELMO domain-containing protein 3,ELMOD3,Homo sapiens,Human,PP4068,RBED1,RBM29,RNA-binding motif and ELMO domain-containing protein 1,RNA-binding motif protein 29,RNA-binding protein 29


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur