Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29674074
Publication Date : //

Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples.


The development of a fluorescent multiplexed microarray platform able to detect and quantify a wide variety of pollutants in seawater is reported. The microarray platform has been manufactured by spotting 6 different bioconjugate competitors and it uses a cocktail of 6 monoclonal or polyclonal antibodies raised against important families of chemical pollutants such as triazine biocide (i.e. Irgarol 1051®), sulfonamide and chloramphenicol antibiotics, polybrominated diphenyl ether flame-retardant (PBDE, i.e. BDE-47), hormone (17β-estradiol), and algae toxin (domoic acid). These contaminants were selected as model analytes, however, the platform developed has the potential to detect a broader group of compounds based on the cross-reactivity of the immunoreagents used. The microarray chip is able to simultaneously determine these families of contaminants directly in seawater samples reaching limits of detection close to the levels found in contaminated areas (Irgarol 1051®, 0.19 ± 0,06 µg L; sulfapyridine, 0.17 ± 0.07 µg L; chloramphenicol, 0.11 ± 0.03 µg L; BDE-47, 2.71 ± 1.13 µg L; 17β-estradiol, 0.94 ± 0.30 µg L and domoic acid, 1.71 ± 0.30 µg L). Performance of the multiplexed microarray chip was assessed by measuring 38 blind spiked seawater samples containing either one of these contaminants or mixtures of them. The accuracy found was very good and the coefficient of variation was < 20% in all the cases. No sample pre-treatment was necessary, and the results could be obtained in just 1 h 30 min. The microarray shows high sample throughput capabilities, being able to measure simultaneously more than 68 samples and screen them for a significant number of chemical contaminants of interest in environmental screening programs.

Authors : Sanchis Ana , Salvador J-Pablo , Campbell Katrina , Elliott Christopher T , Shelver Weilin L , Li Qing X , Marco M-Pilar ,

Related products :

Catalog number Product name Quantity
TM 945 BILE SALTS BRILLIANT GREEN STARCH AGAR (for selective isolation & identification of Aeromonas hydrophila from food & environmental samples.) 500 gm
EIVT-100 EnzyChrom™ Invertase Assay Kit, Quantitative determination of invertase_sucrase activity in biological and environmental (e.g. soil) samples by colorimetric (570nm) or fluorimetric (530_585nm) method. 100tests
EIVT-100 EnzyChrom™ Invertase Assay Kit, Quantitative determination of invertase_sucrase activity in biological and environmental (e.g. soil) samples by colorimetric (570nm) or fluorimetric (530_585nm) method 100Tests
P-1012-1 FitAmp Circulating DNA Quantification Kit (48 samples)
P-1012-1  FitAmp Circulating DNA Quantification Kit (48 samples)
P-1012-2  FitAmp Circulating DNA Quantification Kit (96 samples)
P-1012-2 FitAmp Circulating DNA Quantification Kit (96 samples)
FP-303-CY5 Streptavidin _ Cy5 Assay Kit Fluorescent Detection of Biotinylated Samples 100 assays
FP-303-CY3 Streptavidin _ Cy3 Assay Kit Fluorescent Detection of Biotinylated Samples 100assays
FP-303-CY3 Streptavidin _ Cy3 Assay Kit Fluorescent Detection of Biotinylated Samples 100 assays
FP-303-CY5 Streptavidin _ Cy5 Assay Kit Fluorescent Detection of Biotinylated Samples 100assays
FP-304-CY3 Avidin _ Cy3 Assay Kit Fluorescent Detection of Biotinylated Samples 100assays
FP-304-CY5 Avidin _ Cy5 Assay Kit Fluorescent Detection of Biotinylated Samples 100assays
FP-304-CY3 Avidin _ Cy3 Assay Kit Fluorescent Detection of Biotinylated Samples 100 assays
FP-304-CY5 Avidin _ Cy5 Assay Kit Fluorescent Detection of Biotinylated Samples 100 assays
FP-303-CY3 Biochemicals: Streptavidin - Cy3 Assay Kit Fluorescent Detection of Biotinylated Samples 100assays
FP-304-488 Avidin _ ATTO 488 Assay Kit Fluorescent Detection of Biotinylated Samples 100 assays
FP-304-532 Avidin _ ATTO 532 Assay Kit Fluorescent Detection of Biotinylated Samples 100 assays
FP-304-550 Avidin _ ATTO 550 Assay Kit Fluorescent Detection of Biotinylated Samples 100 assays
FP-303-425 Streptavidin _ ATTO 425 Assay Kit Fluorescent Detection of Biotinylated Samples 100assays
FP-303-590 Streptavidin _ ATTO 590 Assay Kit Fluorescent Detection of Biotinylated Samples 100assays
FP-303-550 Streptavidin _ ATTO 550 Assay Kit Fluorescent Detection of Biotinylated Samples 100assays
FP-303-532 Streptavidin _ ATTO 532 Assay Kit Fluorescent Detection of Biotinylated Samples 100assays
FP-304-590 Avidin _ ATTO 590 Assay Kit Fluorescent Detection of Biotinylated Samples 100 assays
FP-304-655 Avidin _ ATTO 655 Assay Kit Fluorescent Detection of Biotinylated Samples 100 assays


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur