Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29676133
Publication Date : //

[Psoralen inhibits RAW264.7 differentiation into osteoclasts and bone resorption by regulating CD4+T cell differentiation].


This paper aimed to investigate whether psoralen inhibits the differentiation and bone resorption by regulating CD4+T cell differentiation in RANKL-induced osteoclastogenesis in RAW264.7 cells, and elucidate its mechanism for osteoporosis. CD4+T cells were isolated from spleen cells of Balb/c mice by immunomagnetic separation method. The cells were divided into blank control group and psoralen group. The cells were cultured in 24-well plates and cultured for 3 days, and then they were collected for co-culture experiments after 4 days. Co-culture experiments were divided into RAW264.7 cell group, psoralen+RAW264.7 cell group, without psoralen treatment of CD4+T cells+RAW264.7 cell group, psoralen treatment of CD4+T cells+RAW264.7 cell group. After 5 days of co-culture, TRAP staining was used to detect the number of osteoclasts, and after 8 days of co-culture, bone resorption was evaluated by toluidine blue staining. The expressions of RORγt, Foxp3, IL-17, TNF-α, TGF-β and IL-10 in CD4+T cells and osteoclast differentiation-related genes MMP-9, TRAP and Cat-K were detected by Real-time polymerase chain reaction (RT-PCR); ELISA kit was used to detect IL-17, TNF-α, TGF-β and IL-10 and other cytokines levels. Our data confirmed that the psoralen significantly promoted the expression of Foxp3, TGF-β and IL-10 in CD4+T, and inhibited the expression of RORγt, IL-17 and TNF-α in CD4+T, the CD4+T cells without treatment by psoralen can significantly promote RANKL-induced differentiation of RAW264.7 to osteoclasts, and psoralen treatment of CD4+T can significantly inhibit RANKL-induced RAW264.7 osteoclast differentiation and bone resorption. Taken together, psoralen inhibits the differentiation and bone resorption of RAW264.7 into osteoclasts by promoting the development of CD4+ CD25+ Treg/Th17 balance in CD4+T cells to CD4+CD25+T.

Authors : Li Jin-Ping , Xie Bao-Ping , Zhang Wen-Juan , Shi Li-Ying , Li Wei-Juan , Zeng Ying , Gan Guo-Xing , Li Yu-Hong ,

Related products :

Catalog number Product name Quantity
29-195 Ehf is a transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. It may act as a repressor for a specific subset of ETS_AP-1-responsive genes, a 0.05 mg
PNb-031 IL-6 Human Host: Nicotiana benthamiana HGF; HSF; BSF2; IL-6; IFNB2; interleukin 6 (interferon, beta 2); B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation fact 50
PNb-030 IL-6 Human Host: Nicotiana benthamiana HGF; HSF; BSF2; IL-6; IFNB2; interleukin 6 (interferon, beta 2); B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation fact 10
PNb-029 IL-6 Human Host: Nicotiana benthamiana HGF; HSF; BSF2; IL-6; IFNB2; interleukin 6 (interferon, beta 2); B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation fact 5
ASC-ASG-2011-2 Cytokines: Recombinant Human SFRP5(E.Coli), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characterization 250 ug
ASC-ASG-2011-3 Cytokines: Recombinant Human SFRP5(E.Coli), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characterization 1000 ug
ASC-ASG-2005-1 Cytokines: Recombinant Frizzled-5 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 50 ug
ASC-ASG-2011-3 Cytokines: Recombinant Human SFRP5(E.Coli), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characterization 1000 ug
ASC-ASG-1017-3 Cytokines: Recombinant Human flt3 LIGAND, Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characterization 1000
ASC-ASG-1017-3 Cytokines: Recombinant Human flt3 LIGAND, Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characterization 1000
ASC-ASG-2006-3 Cytokines: Recombinant Frizzled-8 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 1000 ug
ASC-ASG-2005-3 Cytokines: Recombinant Frizzled-5 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 1000 ug
ASC-ASG-2011-1 Cytokines: Recombinant Human SFRP5(E.Coli), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characterization 50 ug
ASC-ASG-2006-3 Cytokines: Recombinant Frizzled-8 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 1000 ug
ASC-ASG-2006-2 Cytokines: Recombinant Frizzled-8 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 250 ug
ASC-ASG-1017-2 Cytokines: Recombinant Human flt3 LIGAND, Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characterization 100
ASC-ASG-1017-1 Cytokines: Recombinant Human flt3 LIGAND, Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characterization 10
ASC-ASG-2001-1 Cytokines: Recombinant Human_Mouse Activin A (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 5 ug
ASC-ASG-2001-2 Cytokines: Recombinant Human_Mouse Activin A (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 25 ug
ASC-ASG-2001-3 Cytokines: Recombinant Human_Mouse Activin A (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 100 ug
ASC-ASG-2005-1 Cytokines: Recombinant Frizzled-5 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 50 ug
ASC-ASG-2005-2 Cytokines: Recombinant Frizzled-5 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 250 ug
ASC-ASG-2005-3 Cytokines: Recombinant Frizzled-5 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 1000 ug
ASC-ASG-2006-1 Cytokines: Recombinant Frizzled-8 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 50 ug
ASC-ASG-2006-2 Cytokines: Recombinant Frizzled-8 Fc Fusion (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell C 250 ug


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur