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Pubmed ID :29680034
Publication Date : //

Colloidal gold-based immunochromatographic strip assay for the rapid detection of three natural estrogens in milk.


In this study, we developed highly sensitive and specific monoclonal antibodies (mAbs) against estrone (E), 17β-estradiol (17β-E), and estriol (E). The half-maximal inhibitory concentration values of anti-E, anti-17β-E, and anti-E mAbs were 0.46, 0.36, and 0.39 ng/mL, respectively, based on competitive enzyme-linked immunosorbent assay (ic-ELISA) results. A rapid colloidal gold-based immunoassay strip assay was developed for the determination of E 17β-E, and E residues in milk samples. The assay had a visual cut-off value of 5 ng/mL, and required 10 min to assess with the naked eye. The results obtained from the immunochromatographic strip assay were consistent with those obtained from ic-ELISA and gas chromatography-mass spectrometry. The immunochromatographic strip assay is useful and rapid for the detection of E, 17β-E, and E in milk.

Authors : Wang Zhongxing , Guo Lingling , Liu Liqiang , Kuang Hua , Xu Chuanlai ,

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