Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29695976
Publication Date : //

Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Larvae of Exposed to Azadirachtin Stress Conditions.


Azadirachtin is an efficient and broad-spectrum botanical insecticide against more than 150 kinds of agricultural pests with the effects of mortality, antifeedant and growth regulation. Real-time quantitative polymerase chain reaction (RT-qPCR) could be one of the powerful tools to analyze the gene expression level and investigate the mechanism of azadirachtin at transcriptional level, however, the ideal reference genes are needed to normalize the expression profiling of target genes. In this present study, the fragments of eight candidate reference genes were cloned and identified from the pest . In addition, the expression stability of these genes in different samples from larvae of control and azadirachtin treatments were evaluated by the computational methods of NormFinder, BestKeeper, Delta CT, geNorm, and RefFinder. According to our results, two of the reference genes should be the optimal number for RT-qPCR analysis. Furthermore, the best reference genes for different samples were showed as followed: EF-1α and EF2 for cuticle, β-Tubulin and RPL7A for fat body, EF2 and Actin for midgut, EF2 and RPL13A for larva and RPL13A and RPL7A for all the samples. Our results established a reliable normalization for RT-qPCR experiments in and ensure the data more accurate for the mechanism analysis of azadirachtin.

Authors : Shu Benshui , Zhang Jingjing , Cui Gaofeng , Sun Ranran , Sethuraman Veeran , Yi Xin , Zhong Guohua ,

Related products :

Catalog number Product name Quantity
F5-100FRT Candidosis Real-TM Quant NEW Real Time PCR kit for quantitative detection of C.albicans, C.glabrata, C.kruseC.parapsilosis, C. tropicansi 100 Tests
TB74-100FRT RG, iQ, MX, SC, A, Bacterial Vaginosis Real-TM Quant NSW Multiplex RT-PCR for quantitative detection of G. vaginalis, Atopobium v Lactobacillus spp. and total bacteriae in the vaginal biotope Complete Real Time Test wit 100 tests
DD2 Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2 Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
CL-1001 Real-Time PCR Assay for Monitoring NFkB Regulated Genes
HD-0002-01 HBV Quantitative Real Time PCR Kit DNA I,II 25
HD-0002-02 HBV Quantitative Real Time PCR Kit DNA III, IV 25
R2021 PowerChekTM GMO Screening Real-time PCR Kit (35S, NOS, Plant reference, EPC) 50 rxn
M-430 RT PCR kit Rox 2,5-fold reaction mix for Real Time PCR with antibodies Hot-Start Taq DNA Polymerase and reference dye ROX 200 tests
M-430 RT PCR kit Rox 2,5_fold reaction mix for Real Time PCR with antibodies Hot_Start Taq DNA Polymerase and reference dye ROX 1 kit
R2020 PowerChekTM Universal GMO Screening Real-time PCR Kit (35S, NOS, bar, FMV, Plant reference, EPC) 50 rxn
HD-0003-04 HBV Quantitative & YMDD Mutation Real Time PCR Kit DNA IV 25
HD-0003-02 HBV Quantitative & YMDD Mutation Real Time PCR Kit DNA II 25
HD-0003-01 HBV Quantitative & YMDD Mutation Real Time PCR Kit DNA I 25
HD-0003-03 HBV Quantitative & YMDD Mutation Real Time PCR Kit DNA III 25
HD-0002-02 HBV Quantitative Real Time PCR Kit III, IV *to see for appropriate equipment see catalogue No. MXX_000069 25
HD-0002-01 HBV Quantitative Real Time PCR Kit I, II *to see for appropriate equipment see catalogue No. MXX_000069 25
CL-1001 Single Cell Gene Expression: Real-Time PCR Assay for Monitoring NFkB Regulated Genes
CL-1001 Signaling Cell Gene Expression: Real-Time PCR Assay for Monitoring NFkB Regulated Genes
A-2060 Exicycler 96 Real-Time Quantitative Thermal Block (including PC)
HD-0003-01 HBV Quantitative & YMDD Mutation Real Time PCR Kit I *to see for appropriate equipment see catalogue No. MXX_000069 25
RBIOS2019 Corn (MON 810) 'real time' PCR kit, for the quantitative detection of genetically modified organisms kit
HD-0003-04 HBV Quantitative & YMDD Mutation Real Time PCR Kit IV *to see for appropriate equipment see catalogue No. MXX_000069 25
RBIOS2017 Corn (T25) 'real time' PCR kit, for the quantitative detection of genetically modified organisms kit
RBIOS2020 Corn (35S) 'real time' PCR kit, for the quantitative detection of genetically modified organisms kit


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur