Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29709775
Publication Date : //

Characterization of cathepsin D from Eriocheir sinensis involved in Spiroplasma eriocheiris infection.


Cathepsin D (catD) belongs to a lysosomal aspartic protease superfamily. The full-length catD cDNA from the Chinese mitten crab Eriocheir sinensis (EscatD) was 2748 bp and contained a 1158-bp ORF encoding a protein of 385 amino acids, including a signal peptide and two N-glycosylation sites. Phylogenetic analysis showed that EscatD was clustered into a single group, together with other catD for crustaceans. Quantitative real-time PCR revealed that EscatD was expressed mainly in the eyes, hemocytes, intestine and nerve and was expressed weakly in heart, muscle and gills. After challenge with Spiroplasma eriocheiris, the expression of EscatD was significantly up-regulated from 1 d to 9 d. The copy number of S. eriocheiris in a silencing EscatD group was significantly higher than those in the control groups during S. eriocheiris infection. Meanwhile, the survival rate of crabs decreased in an EscatD-dsRNA group. We further found that knockdown of EscatD by RNA interference resulted in a downward trend of expression levels of JNK, ERK, relish and p38 during the early stage, as well as a reduction in the expression of five antimicrobial peptides genes, namely, crusrin1, crustin2, ALF1, ALF2 and ALF3. The subcellular localization experiment suggested that recombinant EscatD was mainly located in the cytoplasm. The over-expression in Drosophila S2 cells indicated that EscatD could decrease the copy number of S. eriocheiris and increase cell viability. The above results demonstrated that EscatD plays an important immune role in E. sinensis to S. eriocheiris challenge.

Authors : Ning Mingxiao , Yuan Meijun , Liu Min , Gao Qi , Wei Panpan , Gu Wei , Wang Wen , Meng Qingguo ,

Related products :

Catalog number Product name Quantity
27-235 OAS1 is a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. It is induced by interferons and uses adenosine triphosphate in 2'-specifi 0.05 mg
26-604 This protein is a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine t 0.05 mg
32-231 TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other 0.1 mL
E0267h ELISA Cathepsin K,Cathepsin O,Cathepsin O2,Cathepsin X,CTSK,CTSO,CTSO2,Homo sapiens,Human 96T
E0267h ELISA kit Cathepsin K,Cathepsin O,Cathepsin O2,Cathepsin X,CTSK,CTSO,CTSO2,Homo sapiens,Human 96T
U0267h CLIA Cathepsin K,Cathepsin O,Cathepsin O2,Cathepsin X,CTSK,CTSO,CTSO2,Homo sapiens,Human 96T
DEIA1089 Clonorchis sinensis IgG ELISA Kit 96T
DEIA1090 Clonorchis sinensis IgM ELISA Kit 96T
DEIA1090 Clonorchis sinensis IgM ELISA Kit 96T
DEIA1089 Clonorchis sinensis IgG ELISA Kit 96T
DEIA1089 Clonorchis sinensis IgG ELISA Kit 96T
68606-94-0 Orange oil distilled Oleum citrus sinensis ( 1g
CSB-EQ027680HU Human clonorchis sinensis (CS) antibody (IgG) ELISA kit 96T
3327 SPECIFIC IgE ORANGE (Citrus sinensis), SERUM 1
orb60968 Ribavirin Ribavirin(Copegus) is an anti-viral drug indicated for severe RSV infection (individually), hepatitis C infection and other viral infections. For research use only. 50 mg
25-787 RAB38 may be involved in melanosomal transport and docking. Involved in the proper sorting of TYRP1. 0.05 mg
28-991 MYST4 is a histone acetyltransferase which may be involved in both positive and negative regulation of transcription. It is required for RUNX2-dependent transcriptional activation and May be involved 0.05 mg
25-005 The exact function of C2orf28 remains unknown.This gene is thought to be involved in apoptosis, and may also be involved in hematopoietic development and differentiation. Two alternatively spliced tra 0.05 mg
EIAAB05436 Cathepsin L2,Cathepsin U,Cathepsin V,CATL2,CTSL2,CTSU,CTSV,Homo sapiens,Human,UNQ268_PRO305
EIAAB05433 Cathepsin J,Cathepsin L-related protein,Cathepsin P,Catlrp-p,Ctsj,Ctsp,Rat,Rattus norvegicus
EIAAB05434 Cathepsin J,Cathepsin L-related protein,Cathepsin P,Catlrp-p,Ctsj,Ctsp,Mouse,Mus musculus
28-813 PITX1 is a member of the RIEG_PITX homeobox family, which is involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regu 0.1 mg
25-058 PITX1 is a member of the RIEG_PITX homeobox family, which is involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regu 0.05 mg
EIAAB05448 Cathepsin P,Cathepsin X,Cathepsin Z,CTSZ,Homo sapiens,Human
26-012 RHOT1 is mitochondrial GTPase involved in mitochondrial trafficking. It is probably involved in control of anterograde transport of mitochondria and their subcellular distribution. 0.05 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur