Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29717061
Publication Date : //

The interaction between IKKα and LC3 promotes type I interferon production through the TLR9-containing LAPosome.

Toll-like receptor 9 (TLR9) recognizes DNA in endosomes and activates distinct signaling pathways to stimulate the production of proinflammatory cytokines and type I interferons (IFNs). The assembly of signaling platforms on microtubule-associated proteins 1A/1B-light chain 3 (LC3)-decorated endosomal vesicles is required to transduce TLR9 signals that stimulate the production of IFN but not interleukin-12 p40 (IL-12p40). LC3-associated phagocytosis (LAP), a form of noncanonical autophagy, is critical for the activation of interferon regulatory factor 7 (IRF7) and for IFN synthesis. We showed that after the stimulation of TLR9 by CpG oligonucleotides, the autophagy protein LC3 and the kinase IKKα were recruited to endosomes that contained TLR9. The recruitment of IKKα and LC3 to such signaling endosomes was not stimulated by catalysts of classical autophagosome formation but involved LAP formation, which required ATG5 but not FIP200. In addition, we found that the LC3-IKKα complex further associated with both TRAF3 and IRF7. We identified three putative LC3-interacting regions (LIRs) in IKKα, and mutagenesis suggested that two of these were critical for direct binding to LC3. Moreover, mutation of the same LIR sequences failed to rescue type I IFN production in IKKα-deficient dendritic cells upon reconstitution. Together, these data suggest a direct link between LAP formation and IKKα recruitment downstream of TLR9 activation that is necessary to facilitate type I IFN production.

Authors : Hayashi Kachiko , Taura Manabu , Iwasaki Akiko ,

Related products :

Catalog number Product name Quantity
27-005 IFNA7 belongs to the alpha_beta interferon family. IFNA7 is produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes a protein kinase and an o 0.05 mg
26-765 MPP7 acts as an important adapter that promotes epithelial cell polarity and tight junction formation via its interaction with DLG1. MPP7 is involved in the assembly of protein complexes at sites of c 0.05 mg
IFRD1 IFNR Gene interferon production regulator
MD80003 MagCustom project Production Phase, third phase (production phase) of client bead development in production scale incl. SOP, QA, warehousing etc. 1 item
ODN2006-5NC ODN 2006 -Type B-human TLR9 agonist TLR9 agonist containing GpC dinucleotides instead of CpG-antigen grade Species Reactivity: 5 mg Product tipe: ODNs/DNAs
26-077 PSMG1 is a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes a 0.05 mg
TM 985 E.T. MEDIUM (for production of Clostridia for enterotoxin production) 500 gm
LF-MA41484 anti-TLR9(26C593.2), Mouse monoclonal to TLR9, Isotype IgG1 50 ug
3559RBP-50 TLR9 Blocking Peptide target: TLR9 50 μg
PC-TLR9 Rabbit TLR9 Antibody Positive Control 100
DL-TLR9-Hu Human Toll Like Receptor 9 (TLR9) ELISA Kit 96T
P-TLR9 Rabbit TLR9 Antibody Blocking Peptide 100
25-507 KCMF1 belongs to the KCMF1 family. It contains 1 C2H2-type zinc finger and 1 ZZ-type zinc finger. It has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination. 0.05 mg
orb98004 Custom His-tag protein production Biorbyt offers a dedicated service for the production of high quality proteins. We work with an experienced team of biochemists that work on routine and complex pr 1 mg
TLR9 TLR9 Gene toll-like receptor 9
LF-PA43661 Anti-TLR9, Rabbit Polyclonal to TLR9, Isotype , Host Rabbit 50
LF-PA42031 Anti-TLR9, Rabbit Polyclonal to TLR9, Isotype IgG, Host Rabbit 50
20-272-190724 TLR9 - Mouse monoclonal [26C593] to TLR9; CD289 antigen Monoclonal 0.05 mg
LF-PA43407 Anti-TLR9, Goat Polyclonal to TLR9, Isotype , Host Goat 50
orb82717 Human IL12 p70 protein Recombinant Human IL12 is a glycosylated heterodimeric polypeptide containing 503 amino acids. IL12 is a heterodimeric cytokine that stimulates the production of interferon gamm 1 mg
orb41749 Type II 5 phosphatase antibody Polyclonal Rabbit polyclonal to Type II 5 phosphatase. Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in resp 100
YF-MA11537 anti-TLR9 (1E8) type: Primary antibodies host: Mouse 100 ug
LF-MA42704 anti-TLR9 (72-1665) type: Primary antibodies host: Rat 50 ug
ODN1585-1 ODN 1585-Type A murine TLR9 agonist-Antigen grade 1 mg
ODN1668-1 ODN 1668-Type B murine TLR9 Agonist-Antigen grade 1 mg

https://antibody-antibodies.com/ | https://gentaur.com/ | https://gen-script.com/ | https://diagenox.com/ | https://clonagen.com/ | http://gentaursearch.com/ | http://gentaurpub.com/ | https://gentaur-online.com/ | http://anti-anti-pdf.com/ | http://gentaur-worldwide.com/


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur

Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur