Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29735716
Publication Date : //

Reducing resistance allele formation in CRISPR gene drive.


CRISPR homing gene drives can convert heterozygous cells with one copy of the drive allele into homozygotes, thereby enabling super-Mendelian inheritance. Such a mechanism could be used, for example, to rapidly disseminate a genetic payload in a population, promising effective strategies for the control of vector-borne diseases. However, all CRISPR homing gene drives studied in insects thus far have produced significant quantities of resistance alleles that would limit their spread. In this study, we provide an experimental demonstration that multiplexing of guide RNAs can both significantly increase the drive conversion efficiency and reduce germline resistance rates of a CRISPR homing gene drive in We further show that an autosomal drive can achieve drive conversion in the male germline, with no subsequent formation of resistance alleles in embryos through paternal carryover of Cas9. Finally, we find that the promoter significantly lowers somatic Cas9 expression compared with the promoter, suggesting that provides a superior choice in drive strategies where gene disruption in somatic cells could have fitness costs. Comparison of drive parameters among the different constructs developed in this study and a previous study suggests that, while drive conversion and germline resistance rates are similar between different genomic targets, embryo resistance rates can vary significantly. Taken together, our results mark an important step toward developing effective gene drives capable of functioning in natural populations and provide several possible avenues for further control of resistance rates.

Authors : Champer Jackson , Liu Jingxian , Oh Suh Yeon , Reeves Riona , Luthra Anisha , Oakes Nathan , Clark Andrew G , Messer Philipp W ,

Related products :

Catalog number Product name Quantity
M1586 pCAMBIA1105.1 (GusPlus™_lacZ, Hygromycin B_Streptomycin Resistant Plant Expression Vector), Hygromycin B resistance gene for plant selection, streptomycin resistance, GusPlus™ reporter gene, lacZ for
M1586 pCAMBIA1105.1 (GusPlus™ per lacZ, Hygromycin B per Streptomycin Resistant Plant Expression Vector), Hygromycin B resistance gene for plant selection, streptomycin resistance, GusPlus™ reporter gene, l
SUGT1_RAT ELISA Kit FOR Suppressor of G2 allele of SKP1 homolog; organism: Rat; gene name: Sugt1 96T
B761 Sulphate Reducing Medium (TwinPack) USE : For cultivation and enumeration of sulphate reducing bacterium-Thiobacillus thioparus. 5x500gm
TM1095 SULPHATE REDUCING MEDIUM (TRIPLE PACK) (for enumeration of sulphate reducing bacteria in water. 500 gm 1850 100 gm
TM1094 SULPHATE REDUCING MEDIUM (DOUBLE PACK) (for cultivation and enumeration of sulphate reducing Thiobacillus thioparus.) 100 gm
B762 Sulphate Reducing Medium (Triple Pack) USE : For enumeration of sulphate reducing bacteria in water samples. 5x500gm
B762 Sulphate Reducing Medium (Triple Pack) USE For enumeration of sulphate reducing bacteria in water samples. Qty per Litre of Medium: 7.10 of part A + 0.49 of part B + 3.50 of part C
TM1094 SULPHATE REDUCING MEDIUM (DOUBLE PACK) (for cultivation and enumeration of sulphate reducing Thiobacillus thioparus.) 500 gm
B761 Sulphate Reducing Medium (TwinPack) USE For cultivation and enumeration of sulphate reducing bacterium_Thiobacillus thioparus. Qty per Litre of Medium: 2.32 of part A + 10.00 of part B
GCAA_MOUSE ELISA Kit FOR Ig gamma-2A chain C region, A allele; organism: Mouse; gene name: Ighg 96T
SUGT1_MOUSE ELISA Kit FOR Suppressor of G2 allele of SKP1 homolog; organism: Mouse; gene name: Sugt1 96T
KRBBA_MOUSE ELISA Kit FOR Killer cell lectin-like receptor subfamily B member 1B allele A; organism: Mouse; gene name: Klrb1b 96T
KRBBB_MOUSE ELISA Kit FOR Killer cell lectin-like receptor subfamily B member 1B allele B; organism: Mouse; gene name: Klrb1b 96T
26-827 MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. It interferes with erythopoietin-induced erythroid termina 0.05 mg
BUF038A ELISA IgM REDUCING BUFFER, Product Type Accessory Reagent, Specificity ELISA IgM REDUCING BUFFER, Target Species , Host N_A, Format Ready To Use, Isotypes , Applications E, Clone 100 ml
BUF038B ELISA IgM REDUCING BUFFER, Product Type Accessory Reagent, Specificity ELISA IgM REDUCING BUFFER, Target Species , Host N_A, Format Ready To Use, Isotypes , Applications E, Clone 500 ml
SCH-BUF038B ELISA IgM REDUCING BUFFER, Product Type Accessory Reagent, Specificity ELISA IgM REDUCING BUFFER, Target Species , Host N_A, Format Ready To Use, Isotypes , Applications E, Clone 500 ml
BUF038C ELISA IgM REDUCING BUFFER, Product Type Accessory Reagent, Specificity ELISA IgM REDUCING BUFFER, Target Species , Host N_A, Format Ready To Use, Isotypes , Applications E, Clone 1000 ml
SCH-BUF038A ELISA IgM REDUCING BUFFER, Product Type Accessory Reagent, Specificity ELISA IgM REDUCING BUFFER, Target Species , Host N_A, Format Ready To Use, Isotypes , Applications E, Clone 100 ml
SCH-BUF038C ELISA IgM REDUCING BUFFER, Product Type Accessory Reagent, Specificity ELISA IgM REDUCING BUFFER, Target Species , Host N_A, Format Ready To Use, Isotypes , Applications E, Clone 1000 ml
SUGT1 SUGT1 Gene SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
EIAAB25874 Influenza resistance protein,Interferon-induced GTP-binding protein Mx1,Mouse,Mus musculus,Mx1,Myxoma resistance protein 1,Myxovirus resistance protein 1
30-808 PRDX3 is a protein with antioxidant function and is localized in the mitochondrion. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to a 0.05 mg
A-9000-010 CRISPR per Cas9 Monoclonal Antibody 10


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur