Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29792689
Publication Date : //

Selectivity within a Family of Bacterial Phosphothreonine Lyases.


Phosphothreonine lyases are bacterial effector proteins secreted into host cells to facilitate the infection process. This enzyme family catalyzes an irreversible elimination reaction that converts phosphothreonine or phosphoserine to dehydrobutyrine or dehydroalanine, respectively. Herein, we report a study of substrate selectivity for each of the four known phosphothreonine lyases. This was accomplished using a combination of mass spectrometry and enzyme kinetics assays for a series of phosphorylated peptides derived from the mitogen-activated protein kinase (MAPK) activation loop. These studies provide the first experimental evidence that VirA, a putative phosphothreonine lyase identified through homology, is indeed capable of catalyzing phosphate elimination. These studies further demonstrate that OspF is the most promiscuous phosphothreonine lyase, whereas SpvC is the most specific for the MAPK activation loop. Our studies reveal that phospholyases are dramatically more efficient at catalyzing elimination from phosphothreonine than from phosphoserine. Together, our data suggest that each enzyme likely has preferred substrates, either within the MAPK family or beyond. Fully understanding the extent of selectivity is key to understanding the impact of phosphothreonine lyases during bacterial infection and to exploiting their unique chemistry for a range of applications.

Authors : Chambers Kaitlin A , Abularrage Nile S , Scheck Rebecca A ,

Related products :

Catalog number Product name Quantity
26-598 P2RY12 belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine a 0.05 mg
28-668 TAF7I belongs to the family of TATA box binding protein (Tbp)-associated factors. Promoter selectivity for all three classes of eukaryotic RNA polymerases is brought about by multimeric protein comple 0.1 mg
28-759 TAF7I belongs to the family of TATA box binding protein (Tbp)-associated factors. Promoter selectivity for all three classes of eukaryotic RNA polymerases is brought about by multimeric protein comple 0.1 mg
28-643 TAF1b belongs to the family of TATA box binding protein (Tbp)-associated factors. Promoter selectivity for all three classes of eukaryotic RNA polymerases is brought about by multimeric protein comple 0.1 mg
SPC-154F Phosphothreonine Antibody Polyclonal Host: Rabbit Immunogen: Phosphothreonine conjugated to KLH 400ul
SPC-152F Phosphothreonine Antibody: HRP Polyclonal Host: Rabbit Immunogen: Phosphothreonine conjugated to KLH 400ul
SPC-152F Phosphothreonine HRP Polyclonals Ab, Host Rabbit Phosphothreonine conjugated to KLH 400μl
SPC-154F Phosphothreonine Polyclonals Ab, Host Rabbit Phosphothreonine conjugated to KLH 400μl
SMC-158D Phosphothreonine Antibody Monoclonal, Clone number: 18F9 Host: Mouse, Isotype: IgG1 Kappa Immunogen: Phosphothreonine conjugated to KLH 100ul
AHP1333P RABBIT ANTI PHOSPHOTHREONINE HRP, Product Type Polyclonal Antibody, Specificity PHOSPHOTHREONINE, Target Species Synthetic Peptide, Host Rabbit, Format HRP, Isotypes Polyclonal IgG, Applications 50 µg
SCH-AHP1333P RABBIT ANTI PHOSPHOTHREONINE HRP, Product Type Polyclonal Antibody, Specificity PHOSPHOTHREONINE, Target Species Synthetic Peptide, Host Rabbit, Format HRP, Isotypes Polyclonal IgG, Applications 50 µg
AHP1333 RABBIT ANTI PHOSPHOTHREONINE, Product Type Polyclonal Antibody, Specificity PHOSPHOTHREONINE, Target Species Synthetic Peptide, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, Application 50 µg
SCH-AHP1333 RABBIT ANTI PHOSPHOTHREONINE, Product Type Polyclonal Antibody, Specificity PHOSPHOTHREONINE, Target Species Synthetic Peptide, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, Application 50 µg
20-272-192359 Phosphothreonine - Mouse monoclonal [18F6 ] to Phosphothreonine Monoclonal 0.05 ml
SCH-AHP1333B RABBIT ANTI PHOSPHOTHREONINE Biotin, Product Type Polyclonal Antibody, Specificity PHOSPHOTHREONINE, Target Species Synthetic Peptide, Host Rabbit, Format Biotin, Isotypes Polyclonal IgG, Applic 50 µg
AHP1333B RABBIT ANTI PHOSPHOTHREONINE Biotin, Product Type Polyclonal Antibody, Specificity PHOSPHOTHREONINE, Target Species Synthetic Peptide, Host Rabbit, Format Biotin, Isotypes Polyclonal IgG, Applic 50 µg
ODC12-S Anti-Bacterial Ornithine Decarboxylase (ODC) antiserum Species Reactivity: Bacterial 100 ul Product tipe: Antiserum
21614 PhosphoWorks Fluorimetric Pyrophosphate Assay Kit *Enhanced Selectivity* 200 Tests
A1410 A1420 K01 PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit *Enhanced Selectivity* 200 Tests
TM 1146 BLOOD FREE CAMPYLOBACTER SELECTIVITY AGAR BASE (for selective isolation and d 500 gm
TM 1146 BLOOD FREE CAMPYLOBACTER SELECTIVITY AGAR BASE (for selective isolation and d 500 gm
B1303 Rappaport vassiliadis medium USE : For enrichment of Salmonellae, based on its ability to multiply selectivity at high osmotic pressure, low pH and at 43
27-571 RUVBL2 encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional ana 0.05 mg
orb61341 PD 406976 Novel, very potent PKC inhibitor with high selectivity for the A-group of PKC isotypes (alpha, beta and γ), with IC50 values in the 2-10 nM range. For research use only. 5 mg
orb60317 Ki16425 Ki16425 is a LPA receptor inhibitor with selectivity for LPA1 (Ki = 0.34 M)and LPA3(Ki = 0.93 M)vs. LPA2(Ki = 6.5 M) For research use only. 5 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur