Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29863983
Publication Date : //

Mitochondrial Dysfunctions Regulated Radioresistance through Mitochondria-to-Nucleus Retrograde Signaling Pathway of NF-κB/PI3K/AKT2/mTOR.


We investigated the relationship between significantly different genes of the mitochondria-to-nucleus retrograde signaling pathway (RTG) in H1299 ρ cells (mtDNA depleted cell) and compared their radiosensitivity to that of parental ρ cells, to determine the possible intervention targets of radiosensitization. ρ cells were depleted of mitochondrial DNA by chronic culturing in ethidium bromide at low concentration. Radiosensitivity was analyzed using clonogenic assay. Western blot was used to analyze the cell cycle-related proteins, serine/threonine kinase ataxia telangiectasia mutant (ATM), ataxia telangiectasia and Rad3-related protein (ATR) and cyclin B1 (CCNB1). The γ-H2AX foci were detected using confocal fluorescence microscopy. RNA samples were hybridized using the Agilent human genome expression microarray. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used for Gene Ontology (GO) Consortium and pathway annotations of differentially expressed genes, respectively. The H1299 ρ cells were found to be more radioresistant than ρ cells. The ATP production of H1299 ρ cells was lower than that of the ρ cells before or after irradiation. Both H1299 ρ and ρ cells had higher ROS levels after irradiation, however, the radiation-induced ROS production in ρ cells was significantly lower than in ρ cells. In addition, the percentage of apoptosis in H1299 ρ cells was lower than in ρ cells after 6 Gy irradiation. As for the cell cycle and DNA damage response-related proteins ATM, ATR and CCNB1, the expression levels in ρ cells were significantly higher than in ρ cells, and there were less γ-H2AX foci in the ρ than ρ cells after irradiation. Furthermore, the results of the human genome expression microarray demonstrated that the phosphorylated protein levels of the NF-κB/PI3K/AKT2/mTOR signaling pathway were increased after 6 Gy irradiation and were decreased after treatment with the AKT2-specific inhibitor MK-2206 combined with radiation in H1299 ρ cells. MK-2206 treatment also led to an increase in pro-apoptotic proteins. In conclusion, these results demonstrate that mtDNA depletion might activate the mitochondria-to-nucleus retrograde signaling pathway of NF-κB/PI3K/AKT2/mTOR and induce radioresistance in H1299 ρ cells by evoking mitochondrial dysfunctions.

Authors : Wei Yuehua , Chen Lulu , Xu Hui , Xie Conghua , Zhou Yunfeng , Zhou Fuxiang ,

Related products :

Catalog number Product name Quantity
60643 FOXO Reporter Kit (PI3K-AKT Signaling Pathway) 500 reactions
GWB-PS252F FOXO Reporter Kit (PI3K AKT Signaling Pathway)
60511 SRE Reporter Kit (MAPK_ERK Signaling Pathway ),The MAPK_ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular 500 reactions
E0863r Rat ELISA Kit FOR Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial 96T
CSB-EL007386RA Rat Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial(ECSIT) ELISA kit 96T
EIAAB42422 Magmas,Mitochondria-associated granulocyte macrophage CSF-signaling molecule,Mitochondrial import inner membrane translocase subunit TIM16,Mouse,Mus musculus,Pam16,Presequence translocated-associated
CSB-EL007386BO Bovine Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial(ECSIT) ELISA kit 96T
CSB-EL007386MO Mouse Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial(ECSIT) ELISA kit 96T
18-003-43975 Evolutionarily conserved signaling intermediate in Toll pathway. mitochondrial - Protein SITPEC Polyclonal 0.1 mg Protein A
EIAAB12399 Bos taurus,Bovine,ECSIT,Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
EIAAB12401 Ecsit,Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial,Rat,Rattus norvegicus
CSB-EL007386HU Human Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial(ECSIT) ELISA kit 96T
CSB-EL007386RA Rat Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial(ECSIT) ELISA kit SpeciesRat 96T
EIAAB42421 CGI-136,Homo sapiens,Human,MAGMAS,Mitochondria-associated granulocyte macrophage CSF-signaling molecule,Mitochondrial import inner membrane translocase subunit TIM16,PAM16,Presequence translocated-ass
CSB-EL007386BO Bovine Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial(ECSIT) ELISA kit SpeciesBovine 96T
CSB-EL007386HU Human Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial(ECSIT) ELISA kit SpeciesHuman 96T
CSB-EL007386MO Mouse Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial(ECSIT) ELISA kit SpeciesMouse 96T
ECSIT_MOUSE ELISA Kit FOR Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; organism: Mouse; gene name: Ecsit 96T
EIAAB42423 Magmas,Magmas-ps1,Mitochondria-associated granulocyte macrophage CSF-signaling molecule,Mitochondrial import inner membrane translocase subunit TIM16,Pam16,Presequence translocated-associated motor su
EIAAB12400 ECSIT,Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial,Homo sapiens,Human,Protein SITPEC
EIAAB12398 Ecsit,Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial,Mouse,Mus musculus,Protein SITPEC,Sitpec
AA0034 mTOR Signalign Phospho-Specific Array includes 138 highly specific and well-characterized phosphorylation antibodies in the mTOR ignaling pathway. 2 Pieces/Box
20-272-191561 Mitochondria - Mitochondrial Marker. prediluted - Mouse monoclonal [SPM198] to Mitochondria - Mitochondrial Marker. prediluted Monoclonal 7 ml
K868-6 DiscoveryPak™ Hedgehog (Hh) Signaling Pathway Inhibitors Set Hedgehog (Hh) Signaling Pathway Inhibitors Set Inhibitors
A-1009 BEZ-235, PI3K-mTOR inhibitor 100 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur