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Pubmed ID :29915165
Publication Date : //

Effect of Sphingosine-1-Phosphate on Intracellular Free Ca in Cat Esophageal Smooth Muscle Cells.


A comprehensive collection of proteins senses local changes in intracellular Ca concentrations ([Ca]) and transduces these signals into responses to agonists. In the present study, we examined the effect of sphingosine-1-phosphate (S1P) on modulation of intracellular Ca concentrations in cat esophageal smooth muscle cells. To measure [Ca] levels in cat esophageal smooth muscle cells, we used a fluorescence microscopy with the Fura-2 loading method. S1P produced a concentration-dependent increase in [Ca] in the cells. Pretreatment with EGTA, an extracellular Ca chelator, decreased the S1P-induced increase in [Ca], and an L-type Ca-channel blocker, nimodipine, decreased the effect of S1P. This indicates that Ca influx may be required for muscle contraction by S1P. When stimulated with thapsigargin, an intracellular calcium chelator, or 2-Aminoethoxydiphenyl borate (2-APB), an InsP receptor blocker, the S1P-evoked increase in [Ca] was significantly decreased. Treatment with pertussis toxin (PTX), an inhibitor of G-protein, suppressed the increase in [Ca] evoked by S1P. These results suggest that the S1P-induced increase in [Ca] in cat esophageal smooth muscle cells occurs upon the activation of phospholipase C and subsequent release of Ca from the InsP-sensitive Ca pool in the sarcoplasmic reticulum. These results suggest that S1P utilized extracellular Ca via the L type Ca channel, which was dependent on activation of the S1P receptor coupled to PTX-sensitive G protein, via phospholipase C-mediated Ca release from the InsP-sensitive Ca pool in cat esophageal smooth muscle cells.

Authors : Lee Dong Kyu , Min Young Sil , Yoo Seong Su , Shim Hyun Sub , Park Sun Young , Sohn Uy Dong ,

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