Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29925654
Publication Date : //

Neuraminidase-Inhibiting Antibody Titers Correlate with Protection from Heterologous Influenza Virus Strains of the Same Neuraminidase Subtype.


Immune responses induced by currently licensed inactivated influenza vaccines are mainly directed against the hemagglutinin (HA) glycoprotein, the immunodominant antigen of influenza viruses. The resulting antigenic drift of HA requires frequent updating of the vaccine composition and annual revaccination. On the other hand, the levels of antibodies directed against the neuraminidase (NA) glycoprotein, the second major influenza virus antigen, vary greatly. To investigate the potential of the more conserved NA protein for the induction of subtype-specific protection, vesicular stomatitis virus-based replicons expressing a panel of N1 proteins from prototypic seasonal and pandemic H1N1 strains and human H5N1 and H7N9 isolates were generated. Immunization of mice and ferrets with the replicon carrying the matched N1 protein resulted in robust humoral and cellular immune responses and protected against challenge with the homologous influenza virus with an efficacy similar to that of the matched HA protein, illustrating the potential of the NA protein as a vaccine antigen. The extent of protection after immunization with mismatched N1 proteins correlated with the level of cross-reactive neuraminidase-inhibiting antibody titers. Passive serum transfer experiments in mice confirmed that these functional antibodies determine subtype-specific cross-protection. Our findings illustrate the potential of NA-specific immunity for achieving broader protection against antigenic drift variants or newly emerging viruses carrying the same NA but a different HA subtype. Despite the availability of vaccines, annual influenza virus epidemics cause 250,000 to 500,000 deaths worldwide. Currently licensed inactivated vaccines, which are standardized for the amount of the hemagglutinin (HA) antigen, primarily induce strain-specific antibodies, whereas the immune response to the neuraminidase (NA) antigen, which is also present on the viral surface, is usually low. Using NA-expressing single-cycle vesicular stomatitis virus replicons, we show that the NA antigen conferred protection of mice and ferrets against not only the matched influenza virus strains but also viruses carrying NA proteins from other strains of the same subtype. The extent of protection correlated with the level of cross-reactive NA-inhibiting antibodies. This highlights the potential of the NA antigen for the development of more broadly protective influenza vaccines. Such vaccines may also provide partial protection against newly emerging strains with the same NA but a different HA subtype.

Authors : Walz Lisa , Kays Sarah-Katharina , Zimmer Gert , von Messling Veronika ,

Related products :

Catalog number Product name Quantity
X2616P Neuraminidase Neuraminidase (Avian Influenza A (H5N1), Influenza A NA, Neuroaminidase) Polyclonal Antibody 100
X2615P Neuraminidase Neuraminidase (Avian Influenza A (H5N1), Influenza A NA, Neuroaminidase) Polyclonal Antibody 100
orb82551 Influenza A H5N1 Neuraminidase protein Influenza A H5N1 (Avian) Neuraminidase (Middle Region) is an infectious disease antigen_toxin. For research use only. 50
orb82550 Influenza A H5N1 Neuraminidase protein Influenza A H5N1 (Avian) Neuraminidase, C-terminal is an infectious disease antigen_toxin. For research use only. 50
ENEU-100 EnzyChrom™ Neuraminidase Assay Kit, Quantitative determination of neuraminidase activity or screening for neuraminidase inhibitor by colorimetric (570nm) or fluorimetric (530nm_590nm) methods 100Tests
ENEU-100 EnzyChrom™ Neuraminidase Assay Kit, Quantitative determination of neuraminidase activity or screening for neuraminidase inhibitor by colorimetric (570nm) or fluorimetric (530nm_590nm) methods. Procedu 100tests
MAB4237 Influenza B virus neuraminidase monoclonal antibody, clone 602 500 ug
MAB4236 Influenza B virus neuraminidase monoclonal antibody, clone 601 500 ug
MAB4238 Influenza B virus neuraminidase monoclonal antibody, clone 603 500 ug
MD-05-0315 Rabbit Anti-Influenza A Virus Neuraminidase Antibodies 100
MD-05-0319 Mouse Anti-Influenza B Virus Neuraminidase Antibodies 500
'AP30773PU-N Seasonal H1N1 Neuraminidase (Neuraminidase) IgG antibody Ab host: Rabbit 0.1 mg
EIAAB27004 Mouse,Mus musculus,N-acetyl-alpha-neuraminidase 4,Neu4,Neuraminidase 4,Sialidase-4
9001-67-6 Neuraminidase from arthrobacter ureafa Neuraminidase from art 1g
X2615P Neuraminidase (Avian Influenza A (H5N1), Influenza A NA, Neuroaminidase) type: Polyclonal Antibody host: rabbit Isotype: IgG 100
X2616P Neuraminidase (Avian Influenza A (H5N1), Influenza A NA, Neuroaminidase) type: Polyclonal Antibody host: Rabbit Isotype: IgG 100
orb82346 Influenza A N1 Peptide Influenza A Neuraminidase N1 (A_Georgia_20_2006)(H1N1) is an infectious disease antigen_toxin. For research use only. 50
orb82345 Influenza A N1 Peptide Influenza A Neuraminidase N1 (A_Georgia_20_2006)(H1N1) is an infectious disease antigen_toxin. For research use only. 50
orb109501 Influenza A Neuraminidase N1 antibody 100 ug
GWB-6F44A8 Influenza B Neuraminidase, Antibody
orb109500 Influenza A Neuraminidase N1 antibody 100 ug
GWB-E7839B Influenza B Neuraminidase, Antibody
GWB-F47D6E Influenza B Neuraminidase, Antibody
3421 Avian Influenza Neuraminidase Antibody 0.5 mg
3421 Avian Influenza Neuraminidase Antibody 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur