Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29925655
Publication Date : //

Structural Basis for the Broad, Antibody-Mediated Neutralization of H5N1 Influenza Virus.


Human infection with highly pathogenic avian influenza A viruses causes severe disease and fatalities. We previously identified a potent and broadly neutralizing antibody (bnAb), 13D4, against the H5N1 virus. Here, we report the co-crystal structure of 13D4 in complex with the hemagglutinin (HA) of A/Vietnam/1194/2004 (H5N1). We show that heavy-chain complementarity-determining region 3 (HCDR3) of 13D4 confers broad yet specific neutralization against H5N1, undergoing conformational rearrangement to bind to the receptor binding site (RBS). Further, we show that mutating four critical residues within the RBS-Trp153, Lys156, Lys193, and Leu194-disrupts the binding between 13D4 and HA. Viruses bearing Asn193 instead of Lys/Arg can evade 13D4 neutralization, indicating that Lys193 polymorphism might be, at least in part, involved in the antigenicity of recent H5 genotypes (such as H5N6 and H5N8) as distinguished from H5N1. BnAb 13D4 may offers a template for therapeutic RBS inhibitor design and serve as an indicator of antigenic change for current H5 viruses. Infection by highly pathogenic avian influenza A virus remains a threat to public health. Our broadly neutralizing antibody, 13D4, is capable of neutralizing all representative H5N1 viruses and protecting mice against lethal challenge. Structural analysis revealed that 13D4 uses heavy-chain complementarity-determining region 3 (HCDR3) to fit the receptor binding site (RBS) via conformational rearrangement. Four conserved residues within the RBS are critical for the broad potency of 13D4. Importantly, polymorphism of Lys193 on the RBS may be associated with the antigenicity shift from H5N1 to other newly emerging viruses, such as H5N6 and H5N8. Our findings may pave the way for highly pathogenic avian influenza virus vaccine development and therapeutic RBS inhibitor design.

Authors : Lin Qingshan , Li Tingting , Chen Yixin , Lau Siu-Ying , Wei Minxi , Zhang Yuyun , Zhang Zhenyong , Yao Qiaobin , Li Jinjin , Li Zhihai , Wang Daning , Zheng Qingbing , Yu Hai , Gu Ying , Zhang Jun , Chen Honglin , Li Shaowei , Xia Ningshao ,

Related products :

Catalog number Product name Quantity
H5N11-C Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A_chicken_India_NIV33487_2006) (17-531aa) protein control for Western Species Reactivity: Influenza Virus 100 ul Product tipe: Pure protein
H5N11-S Anti-Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A_chicken_India_NIV33487_2006) (17-531aa) protein antiserum Species Reactivity: Influenza Virus 100 ul Product tipe: Antbodies
H5N15-R-100 Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A_chicken_India_NIV33487_2006) (17-531aa), His-tag Species Reactivity: Influenza Virus 100 ug Product tipe: Pure protein
H5N15-R-10 Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A_chicken_India_NIV33487_2006) (17-531aa), His-tag Species Reactivity: Influenza Virus 10 ug Product tipe: Pure protein
iha-032 Recombinant Hemagglutinin-Influenza A Virus H5N1 Indonesia 05_2005 VIRAL ANTIGENS- INFLUENZA VIRUS 10
iha-032 Recombinant Hemagglutinin-Influenza A Virus H5N1 Indonesia 05_2005 VIRAL ANTIGENS- INFLUENZA VIRUS 2
iha-035 Recombinant Hemagglutinin-Influenza A Virus H5N1 Vietnam HN31242_2007 VIRAL ANTIGENS- INFLUENZA VIRUS 2
iha-010 Recombinant Hemagglutinin-Influenza A Virus H5N1 Vietnam 1203_04 VIRAL ANTIGENS- INFLUENZA VIRUS 2
iha-010 Recombinant Hemagglutinin-Influenza A Virus H5N1 Vietnam 1203_04 VIRAL ANTIGENS- INFLUENZA VIRUS 10
iha-035 Recombinant Hemagglutinin-Influenza A Virus H5N1 Vietnam HN31242_2007 VIRAL ANTIGENS- INFLUENZA VIRUS 10
iha-032 Recombinant Hemagglutinin-Influenza A Virus H5N1 Indonesia 05_2005 VIRAL ANTIGENS- INFLUENZA VIRUS 100
iha-035 Recombinant Hemagglutinin-Influenza A Virus H5N1 Vietnam HN31242_2007 VIRAL ANTIGENS- INFLUENZA VIRUS 100
iha-010 Recombinant Hemagglutinin-Influenza A Virus H5N1 Vietnam 1203_04 VIRAL ANTIGENS- INFLUENZA VIRUS 100
H5N15-R-10 Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A chicken India NIV33487 2006) (17-531aa), His-tag 10 ug
H5N11-S Anti-Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A chicken India NIV33487 2006) (17-531aa) protein antiserum 100 ul
H5N11-C Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A chicken India NIV33487 2006) (17-531aa) protein control for Western 100 ul
orb81780 Hemagglutinin-Influenza Virus H5N1 Vietnam 1203_04 protein Recombinant Full-Length H5N1 A_Vietnam_1203_04 is glycosylated with N-linked sugars, produced using baculovirus and when processed under dena 2
orb81781 Hemagglutinin-Influenza Virus H5N1 Indonesia 05_2005 protein Recombinant Full-Length H5N1 A_Indonedia_05_2005 is glycosylated with N-linked sugars, produced using baculovirus vectors in insect cells a 2
MD-14-0295P Influenza A H5N1 (Avian) Hemagglutinin (Mid-molecule) Influenza A (H5N1) HA Peptide Proteins 50
MD-14-0294P Influenza A H5N1 (Avian) Hemagglutinin (Mid-molecule) Influenza A (H5N1) HA Peptide Proteins 50
MD-14-0293P Influenza A H5N1 (Avian) Hemagglutinin (Mid-molecule) Influenza A (H5N1) HA Peptide Proteins 50
GWB-9DF258 Influenza A virus non structural (NS) protein, Antibody
orb82554 Influenza A H5N1 Hemagglutinin protein Influenza A H5N1 (Avian) Hemagglutinin (Middle Region) is an infectious disease antigen_toxin. For research use only. 50
orb82553 Influenza A H5N1 Hemagglutinin protein Influenza A H5N1 (Avian) Hemagglutinin (Middle Region) is an infectious disease antigen_toxin. For research use only. 50
orb82556 Influenza A H5N1 Hemagglutinin protein Influenza A H5N1 (Avian) Hemagglutinin (Middle Region) is an infectious disease antigen_toxin. For research use only. 50


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur