Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :30001863
Publication Date : //

Interleukin 29 inhibits RANKL-induced osteoclastogenesis via activation of JNK and STAT, and inhibition of NF-κB and NFATc1.


Interleukin (IL)-29 is known to modulate immune functions of monocytes or macrophages. In this study, we investigated the effect and its underlying mechanism of IL-29 on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastogenesis using murine macrophage cell line RAW264.7 cells and bone-marrow-derived monocyte/macrophage precursor cells (BMMs), and human peripheral blood mononuclear cells (PBMCs). In response to human recombinant IL-29, cell viability and apoptosis were assessed by Cell Counting Kit-8 and flow cytometry; the osteoclast formation and activity by tartrate-resistant acid phosphatase (TRAP) staining and pit formation assay, respectively; the expression and activation of molecules that associated with osteoclastogenesis by real time-PCR, immunoblotting or immunofluorescent analysis. IL-28 receptor α (IL-28Rα), a specific receptor of IL-29 was expressed on RAW264.7 cells. Although IL-29 did not affect the viability and apoptosis of RAW264.7 cells, it inhibited multinucleated cells in the differentiation of osteoclastogenesis, the bone-resorbing activity of mature osteoclasts and osteoclastic specific genes expression including TRAP, cathepsin K (CTSK), nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), C-Fos and matrix metallopeptidase 9 (MMP-9). This inhibitory effect of IL-29 was confirmed on BMMs and PBMCs and mediated via IL-28Rα through the activation of Stat1 and 3 and the suppression of nuclear factor kappa B (NF-κB) and NFATc1 nuclear translocation in RAW264.7 cells. In conclusion, IL-29 inhibited osteoclastogenesis via activation of STAT signaling pathway, prevention of NF-κB activation and NFATc1 translocation, and suppression of downstream osteoclastogenic genes expression.

Authors : Peng Qiuyue , Luo Aishu , Zhou Zihao , Xuan Wenhua , Qiu Ming , Wu Qin , Xu Lingxiao , Kong Xiangqing , Zhang Miaojia , Tan Wenfeng , Xue Meilang , Wang Fang ,

Related products :

Catalog number Product name Quantity
E0855r ELISA kit ODF,OPGL,Opgl,Osteoclast differentiation factor,Osteoprotegerin ligand,RANKL,Rankl,Rat,Rattus norvegicus,Receptor activator of nuclear factor kappa-B ligand,TNF-related activation-induced c 96T
U0855r CLIA ODF,OPGL,Opgl,Osteoclast differentiation factor,Osteoprotegerin ligand,RANKL,Rankl,Rat,Rattus norvegicus,Receptor activator of nuclear factor kappa-B ligand,TNF-related activation-induced cytokin 96T
E0855m ELISA Mouse,Mus musculus,ODF,OPGL,Opgl,Osteoclast differentiation factor,Osteoprotegerin ligand,RANKL,Rankl,Receptor activator of nuclear factor kappa-B ligand,TNF-related activation-induced cytokine, 96T
E0855m ELISA kit Mouse,Mus musculus,ODF,OPGL,Opgl,Osteoclast differentiation factor,Osteoprotegerin ligand,RANKL,Rankl,Receptor activator of nuclear factor kappa-B ligand,TNF-related activation-induced cyto 96T
U0855m CLIA Mouse,Mus musculus,ODF,OPGL,Opgl,Osteoclast differentiation factor,Osteoprotegerin ligand,RANKL,Rankl,Receptor activator of nuclear factor kappa-B ligand,TNF-related activation-induced cytokine,T 96T
E0855r ELISA ODF,OPGL,Opgl,Osteoclast differentiation factor,Osteoprotegerin ligand,RANKL,Rankl,Rat,Rattus norvegicus,Receptor activator of nuclear factor kappa-B ligand,TNF-related activation-induced cytoki 96T
E0855h ELISA kit Homo sapiens,Human,ODF,OPGL,OPGL,Osteoclast differentiation factor,Osteoprotegerin ligand,RANKL,RANKL,Receptor activator of nuclear factor kappa-B ligand,TNF-related activation-induced cyto 96T
E0855h ELISA Homo sapiens,Human,ODF,OPGL,OPGL,Osteoclast differentiation factor,Osteoprotegerin ligand,RANKL,RANKL,Receptor activator of nuclear factor kappa-B ligand,TNF-related activation-induced cytokine, 96T
U0855h CLIA Homo sapiens,Human,ODF,OPGL,OPGL,Osteoclast differentiation factor,Osteoprotegerin ligand,RANKL,RANKL,Receptor activator of nuclear factor kappa-B ligand,TNF-related activation-induced cytokine,T 96T
30-121 C21orf33 inhibits both estrogen- and heregulin-beta1-stimulated growth of breast cancer cells, and further suggests that growth inhibition induced in these cells by all-trans retinoic acid occurs via 0.1 mg
LF-PA41076 anti-STAT Induced STAT Inhibitor-2 (SOCS2) , Rabbit polyclonal to STAT Induced STAT Inhibitor-2 (SOCS2) , Isotype IgG, Host Rabbit 50 ug
R20-020 RANKL, soluble Rat Host: E. coli soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor), Tumor necrosis fac 10
OBT1307 SOCS3, SSI_3 (STAT induced STAT inhibitor 3), CIS3, Goat anti_ 100 µg.
OBT1309 SOCS2, STATI2 (STAT induced STAT inhibitor_2), CIS2, Goat anti_ 100 µg.
31-050 MDM4 inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. It inhibits degradation of MDM2. It can reverse MDM2-targeted degradation of p53 w 0.05 mg
31-051 MDM4 inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. inhibits degradation of MDM2. It can reverse MDM2-targeted degradation of p53 whil 0.1 mg
18-783-78901 RABBIT ANTI HUMAN CD254 Biotin - sRANKL; Receptor activator of nuclear factor kappa B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; Osteoprotegerin ligand; OPGL; Osteoclast different 0.05 mg
18-783-75591 RABBIT ANTI HUMAN CD254 - sRANKL; Receptor activator of nuclear factor kappa B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; Osteoprotegerin ligand; OPGL; Osteoclast differentiation 0.1 mg
GWB-E3088D STAT Induced STAT Inhibitor-2 (SOCS2) Rabbit anti-Human Polyclonal (N-Terminus) Antibody
10-663-45158 Soluble TNF Receptor Inhibitor_Fc chimera (TNFRSF1A) Human - Receptor activator of nuclear factor kappa B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; Osteoprotegerin ligand; OPGL; 0.01 mg
10-663-45158 Soluble TNF Receptor Inhibitor_Fc chimera (TNFRSF1A) Human - Receptor activator of nuclear factor kappa B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; Osteoprotegerin ligand; OPGL; 1 mg
10-663-45158 Soluble TNF Receptor Inhibitor_Fc chimera (TNFRSF1A) Human - Receptor activator of nuclear factor kappa B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; Osteoprotegerin ligand; OPGL; 0.05 mg
20-272-190728 sRANKL - Mouse monoclonal [12A380] to sRANKL; Receptor activator of nuclear factor kappa B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; Osteoprotegerin ligand; OPGL; Osteoclast diff 0.05 mg
EIAAB39188 Cish1,JAB,JAK-binding protein,Mouse,Mus musculus,Socs1,SOCS-1,Ssi1,SSI-1,STAT-induced STAT inhibitor 1,Suppressor of cytokine signaling 1
18-003-43725 Suppressor of cytokine signaling 1 - SOCS-1; JAK-binding protein; JAB; STAT-induced STAT inhibitor 1; SSI-1; Tec-interacting protein 3; TIP-3 Polyclonal 0.1 mg Protein A


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur