Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :30008324
Publication Date : //

Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells.


We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes' expression compared with the flat monolayer cells. We further demonstrated that cell-matrix adhesion mediates the interaction between cell morphology and expression of KLF4 and KLF5 through a serum response factor (SRF)-based regulatory double loop. Our results provide a mechanistic view on the coupling among adhesion, stem cell morphology, and pluripotency, shedding light on the critical role of cell-matrix adhesion in the induction and maintenance of hPSC.

Authors : Yu Leqian , Li Junjun , Hong Jiayin , Takashima Yasuhiro , Fujimoto Nanae , Nakajima Minako , Yamamoto Akihisa , Dong Xiaofeng , Dang Yujiao , Hou Yu , Yang Wei , Minami Itsunari , Okita Keisuke , Tanaka Motomu , Luo Chunxiong , Tang Fuchou , Chen Yong , Tang Chao , Kotera Hidetoshi , Liu Li ,

Related products :

Catalog number Product name Quantity
orb84785 IL3 protein Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as va 10
ASC-ASG-2002-1 Cytokines: Recombinant Human BMP4 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteriza 5 ug
ASC-ASG-2013-3 Cytokines: Recombinant Human TGF-beta1 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Charac 100 ug
ASC-ASG-2013-2 Cytokines: Recombinant Human TGF-beta1 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Charac 25 ug
ASC-ASG-2013-1 Cytokines: Recombinant Human TGF-beta1 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Charac 5 ug
ASC-ASG-2012-2 Cytokines: Recombinant Human Sonic Hedgehog (Human cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Ch 25 ug
ASC-ASG-2012-1 Cytokines: Recombinant Human Sonic Hedgehog (Human cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Ch 5 ug
ASC-ASG-2010-3 Cytokines: Recombinant Human Noggin (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteri 1000 ug
ASC-ASG-2002-2 Cytokines: Recombinant Human BMP4 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteriza 25 ug
ASC-ASG-2010-1 Cytokines: Recombinant Human Noggin (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteri 50 ug
ASC-ASG-2010-2 Cytokines: Recombinant Human Noggin (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteri 250 ug
ASC-ASG-2013-3 Cytokines: Recombinant Human TGF-beta1 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Charac 100 ug
ASC-ASG-2010-3 Cytokines: Recombinant Human Noggin (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteri 1000 ug
ASC-ASG-2002-3 Cytokines: Recombinant Human BMP4 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteriza 100 ug
ASC-ASG-2012-3 Cytokines: Recombinant Human Sonic Hedgehog (Human cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Ch 100 ug
ASC-ASG-2012-3 Cytokines: Recombinant Human Sonic Hedgehog (Human cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Ch 100 ug
ASC-ASG-2010-1 Cytokines: Recombinant Human Noggin (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteri 50 ug
ASC-ASG-2013-1 Cytokines: Recombinant Human TGF-beta1 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Charac 5 ug
ASC-ASG-2002-1 Cytokines: Recombinant Human BMP4 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteriza 5 ug
ASC-ASG-2012-2 Cytokines: Recombinant Human Sonic Hedgehog (Human cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Ch 25 ug
ASC-ASG-2012-1 Cytokines: Recombinant Human Sonic Hedgehog (Human cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Ch 5 ug
ASC-ASG-2002-2 Cytokines: Recombinant Human BMP4 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteriza 25 ug
ASC-ASG-2010-2 Cytokines: Recombinant Human Noggin (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteri 250 ug
ASC-ASG-2013-2 Cytokines: Recombinant Human TGF-beta1 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Charac 25 ug
ASC-ASG-2002-3 Cytokines: Recombinant Human BMP4 (Human Cells), Stem Cell Growth Factors and Differentiation Factors for Reprogramming and Stem Cell Expansion, Generation, Differentiation and Stem Cell Characteriza 100 ug


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur