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Pubmed ID :30087169
Publication Date : //

Investigating the Campylobacter jejuni Transcriptional Response to Host Intestinal Extracts Reveals the Involvement of a Widely Conserved Iron Uptake System.


is a pathogenic bacterium that causes gastroenteritis in humans yet is a widespread commensal in wild and domestic animals, particularly poultry. Using RNA sequencing, we assessed transcriptional responses to medium supplemented with human fecal versus chicken cecal extracts and in extract-supplemented medium versus medium alone. exposed to extracts had altered expression of 40 genes related to iron uptake, metabolism, chemotaxis, energy production, and osmotic stress response. In human fecal versus chicken cecal extracts, displayed higher expression of genes involved in respiration () and in known or putative iron uptake systems (, , , and [here designated ]). The genes and downstream overlapping gene were investigated further. Uncharacterized homologues of this system were identified in 33 diverse bacterial species representing 6 different phyla, 21 of which are associated with human disease. The and () genes encode an iron transporter and a periplasmic iron binding protein, respectively; however, the role of the downstream genes was unknown. A Δ- deletion strain had an iron-sensitive phenotype, consistent with a previously characterized Δ mutant, and showed reduced growth in acidic medium, increased sensitivity to streptomycin, and higher resistance to HO stress. In iron-restricted medium, the and genes were required for optimal growth when using human fecal extracts as an iron source. Collectively, this implicates a function for the gene cluster in iron scavenging and stress survival in the human intestinal environment. Direct comparative studies of infection of a zoonotic commensal host and a disease-susceptible host are crucial to understanding the causes of infection outcome in humans. These studies are hampered by the lack of a disease-susceptible animal model reliably displaying a similar pathology to human campylobacteriosis. In this work, we compared the phenotypic and transcriptional responses of to intestinal compositions of humans (disease-susceptible host) and chickens (zoonotic host) by using human fecal and chicken cecal extracts. The mammalian gut is a complex and dynamic system containing thousands of metabolites that contribute to host health and modulate pathogen activity. We identified genes more highly expressed during exposure to human fecal extracts in comparison to chicken cecal extracts and differentially expressed in extracts compared with medium alone, and targeted one specific iron uptake system for further molecular, genetic, and phenotypic study.

Authors : Liu Martha M , Boinett Christine J , Chan Anson C K , Parkhill Julian , Murphy Michael E P , Gaynor Erin C ,

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