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Pubmed ID :30234444
Publication Date : //

Optimization of the Platelet-rich Plasma Concentration for Mesenchymal Stem Cell Applications.


Platelet-rich plasma (PRP) and mesenchymal stem cells (MSCs) are promising tools for muscular-skeletal regeneration. However, increasing evidence has demonstrated controversial effects of PRP on the tissue regeneration. To obtain optimum PRP concentrations for MSC expansion and to accurately control osteogenic, adipocytic and chondrogenic differentiation, MSCs were exposed to PRP alone or in combination with induction medium. We found that PRPs with the platelet concentration beyond 1500×109 pl/L were preferable to promote MSC proliferation. Additionally, PRPs ranging from 200×109 to 3000×109 pl/L were capable of augmenting MSC osteogenesis and PRP with 1500×109 pl/L was most effective for MSC osteogenic differentiation. Furthermore, PRPs in low platelet concentration range just slightly promoted MSC adipogenesis, and only when the platelet concentration was beyond 1800×109 pl/L, the promoted effects were evident. Moreover, PRPs range from 1000 to 3000×109 pl/L significantly enhanced chondrogenesis of MSCs in the absence and presence of chondrogenic induction medium, and PRP with 2000×109 pl/L were more effective for MSC chondrogenesis. Further, we explored the mechanisms of PRP-induced MSCs differentiation, showing that the growth factors played a major role in this process while other unknown factors may also involved in it. At last, we measured the levels of cytokines to learn that PRP-treatment suppressed the secretion of interleukin-1β, interleukin-6 and tumor necrosis factor-α but favored the production of interleukin-10 by MSCs. In summary, our findings demonstrated that PRPs with different concentrations of platelets exerted different effects on proliferation and differentiation of MSCs, which indicated that preparing appropriate PRPs may be a precise and efficient strategy for improving MSC-based tissue regeneration.

Authors : Ketao Wang , Zhongli Li , Ji Li , Weixiong Liao , Yuanyuan Qin , Ning Zhang , Xiulin Huo , Ning Mao , Zhu Heng ,

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