Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :30240782
Publication Date : //

Acute inflammation sensitizes knee-innervating sensory neurons and decreases mouse digging behavior in a TRPV1-dependent manner.

Ongoing, spontaneous pain is characteristic of inflammatory joint pain and reduces an individual's quality of life. To understand the neural basis of inflammatory joint pain, we made a unilateral knee injection of complete Freund's adjuvant (CFA) in mice, which reduced their natural digging behavior. We hypothesized that sensitization of knee-innervating dorsal root ganglion (DRG) neurons underlies this altered behavior. To test this hypothesis, we performed electrophysiological recordings on retrograde labeled knee-innervating primary DRG neuron cultures and measured their responses to a number of electrical and chemical stimuli. We found that 24-h after CFA-induced knee inflammation, knee neurons show a decreased action potential generation threshold, as well as increased GABA and capsaicin sensitivity, but have unaltered acid sensitivity. The inflammation-induced sensitization of knee neurons persisted for 24-h in culture, but was not observed after 48-h in culture. Through immunohistochemistry, we showed that the increased knee neuron capsaicin sensitivity correlated with enhanced expression of the capsaicin receptor, transient receptor potential vanilloid 1 (TRPV1) in knee-innervating neurons of the CFA-injected side. We also observed an increase in the co-expression of TRPV1 with tropomyosin receptor kinase A (TrkA), which is the receptor for nerve growth factor (NGF), suggesting that NGF partially induces the increased TRPV1 expression. Lastly, we found that systemic administration of the TRPV1 antagonist, A-425619, reversed the decrease in digging behavior induced by CFA injection, further confirming the role of TRPV1, expressed by knee neurons, in acute inflammatory joint pain.

Authors : Chakrabarti Sampurna , Pattison Luke A , Singhal Kaajal , Hockley James R F , Callejo Gerard , Smith Ewan St John ,

Related products :

Catalog number Product name Quantity
Y090132 Anti_chaoptin, sensory neurons Monoclonal 100ul
Y090132 Anti-chaoptin, sensory neurons Monoclonal Antibody 100ul
4-20354 Neural Dissociation System 4 (Cerebella neurons Dorsa horn neurons Neurons, sympathetic Precursor Spinal), Mouse and Rat ea
26-524 FEZF1 is a transcription repressor. It is involved in the axonal projection and proper termination of olfactory sensory neurons (OSN). It plays a role in rostro-caudal patterning of the diencephalon a 0.05 mg
26-240 DRGX is a transcription factor required for the formation of correct projections from nociceptive sensory neurons to the dorsal horn of the spinal cord and normal perception of pain. 0.05 mg
26-202 This protein can induce apoptosis in a caspase-dependent manner and plays a role in p53_TP53-dependent apoptosis. 0.05 mg
26-112 PIP3-E enhances the promotion of guanine-nucleotide exchange by PSCD2 on ARF6 in a concentration-dependent manner. 0.05 mg
30-179 PIP3-E enhances the promotion of guanine-nucleotide exchange by PSCD2 on ARF6 in a concentration-dependent manner. 0.1 mg
4-20352 Neural Dissociation System 2 (Neurons, Neurons and glial,Retina), Mouse and Rat ea
4-20355 Neural Dissociation System 5 (Cortical Ganglion, retina Neurons, hippocampal Neurons, visual cortex), Mouse and Rat ea
26-251 MTA3 plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin le 0.05 mg
DL-TRPV1-Mu Mouse Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit 96T
28-997 BRD7 is an activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. It induces dephosphorylation of GSK3B at 'Tyr-216'. 0.05 mg
26-448 Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterog 0.05 mg
QAM-INF-1-4 Mouse Inflammation Array Q1 (4) Antibody Arrays Inflammation Arrays 32-50 Sample Kit
AAM-INF-G1-8 Mouse Inflammation Array G1 (8) Antibody Arrays Inflammation Arrays 8 Sample Kit
QAM-INF-1-2 Mouse Inflammation Array Q1 (2) Antibody Arrays Inflammation Arrays 16-22 Sample Kit
AAM-INF-1-8 Mouse Inflammation Array C1 (8) Antibody Arrays Inflammation Arrays 8 Sample Kit
AAM-INF-G1-4 Mouse Inflammation Array G1 (4) Antibody Arrays Inflammation Arrays 4 Sample Kit
AAM-INF-1-4 Mouse Inflammation Array C1 (4) Antibody Arrays Inflammation Arrays 4 Sample Kit
QAM-INF-1-1 Mouse Inflammation Array Q1 (1) Antibody Arrays Inflammation Arrays 1-8 Sample Kit
29-120 MLXIPL is a basic helix-loop-helix leucine zipper transcription factor of the Myc_Max_Mad superfamily. This protein forms a heterodimeric complex and binds and activates, in a glucose-dependent manner 0.1 mg
30-094 Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and s 0.1 mg
25-829 UBE2S is a member of the ubiquitin-conjugating enzyme family. It is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ub 0.05 mg
26-995 IRF2BP1 acts as a transcriptional repressor. IRF2BP1 also acts as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase acti 0.05 mg


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017


france@gentaur.com | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur