Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :30244119
Publication Date : //

TRPV1 mediates capsaicin-stimulated metabolic activity but not cell death or inhibition of interleukin-1β release in human THP-1 monocytes.


Human monocytes and dendritic cells express transient receptor potential vanilloid 1 (TRPV1) which may play a role in mediating the inflammatory, immune and cancer surveillance responses of these cells. The aim of the present study was to investigate TRPV1 expression and function in THP-1 monocytic cells. RT-PCR and Western blot were used to detect TRPV1. The metabolic activity and viability of THP-1 cells following exposure to vanilloids was assessed using resorufin production from rezazurin. Cytokine release was measured using ELISA. TRPV1 was expressed in cultured THP-1 monocytic cells and naïve monocytes. Lower concentrations (<250 μM) of capsaicin, but not other putative TRPV1 agonists, were shown to stimulate cell metabolic activity, whereas at concentrations >250 μM, all agonists decreased metabolic activity. Capsaicin-stimulated THP-1 metabolic activity was blocked by the TRPV1 antagonist, 5-iodo-resiniferatoxin (5'-IRTX), whereas the decline in resorufin production by THP-1 cells at higher capsaicin concentrations (due to cell death), was not affected by 5'-IRTX. Finally, capsaicin (≤125 μM) significantly increased lipopolysaccharide-stimulated IL-6 and TNF-α release from THP-1 cells, whereas phytohaemagglutinin-stimulated IL-1β, TNF-α, MCP-1 and IL-6 release were concentration-dependently inhibited by capsaicin. Modulation of IL-1β release was not TRPV1 mediated. Overall, these results show that functional TRPV1 channels are present in naïve monocytes and THP-1 cells, and when activated, increase cell metabolic activity. In addition, capsaicin modifies cytokine release from THP-1 cells and induces cell death, most likely by a mechanism that is independent of TRPV1 activation.

Authors : Kunde Dale A , Yingchoncharoen Januttha , Jurković Saša , Geraghty Dominic P ,

Related products :

Catalog number Product name Quantity
EIAAB44117 Capsaicin receptor,Homo sapiens,Human,Osm-9-like TRP channel 1,OTRPC1,Transient receptor potential cation channel subfamily V member 1,TrpV1,TRPV1,Vanilloid receptor 1,VR1
31-008 The protein encoded by FADD is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recr 0.05 mg
M1377 MarkerGene™ LysoLive™ Lysosomal Sulfatase Assay Kit, Useful for labeling lysosomes in a live-cell format and capable of monitoring lysosomal metabolic activity, 1 kit
M1376 MarkerGene™ LysoLive™ Lysosomal Phosphatase Assay Kit, Useful for labeling lysosomes in a live-cell format and capable of monitoring lysosomal metabolic activity, 1 kit
29-816 Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration a 0.05 mg
EIAAB44113 Capsaicin receptor,Osm-9-like TRP channel 1,OTRPC1,Rat,Rattus norvegicus,Transient receptor potential cation channel subfamily V member 1,TrpV1,Trpv1,Vanilloid receptor 1,Vanilloid receptor type 1-lik
30-126 TCP10L is a human leucine zipper protein with transcription inhibition activity. 0.1 mg
26-459 This protein mediates receptor activity 0.05 mg
EIAAB07401 Cell death activator CIDE-B,Cell death-inducing DFFA-like effector B,CIDEB,Homo sapiens,Human
EIAAB07398 Cell death activator CIDE-A,Cell death-inducing DFFA-like effector A,CIDEA,Homo sapiens,Human
31-102 GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the gaba_benzodiazepine receptor and opening an integral chloride channel. 0.1 mg
31-103 GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the gaba_benzodiazepine receptor and opening an integral chloride channel. 0.1 mg
31-134 GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the gaba_benzodiazepine receptor and opening an integral chloride channel. 0.1 mg
26-477 ORAI2 is a multi-pass membrane protein, and it belongs to the Orai family. It is a Ca (2+) release-activated Ca (2+)-like (CRAC-like) channel subunit which mediates Ca (2+) influx and increase in Ca ( 0.05 mg
30-574 ORAI2 is a multi-pass membrane protein, and it belongs to the Orai family. It is a Ca (2+) release-activated Ca (2+)-like (CRAC-like) channel subunit which mediates Ca (2+) influx and increase in Ca ( 0.05 mg
25-256 BTBD10 appears to behave as a suppressor of cell death including neuronal cell death related to amyotrophic lateral sclerosis and an enhancer of cell growth via its positive regulation of Akt phosphor 0.05 mg
29-910 SLC29A2 mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. It is less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyrida 0.1 mg
P-4003-48 EpiQuik HAT Activity per Inhibition Assay Kit 48 assays
P-4003-96 EpiQuik HAT Activity per Inhibition Assay Kit 96 assays
orb61265 MK-4827 MK-4827 displays excellent PARP 1 and 2 inhibition with IC50 = 3.8 and 2.1 nM, resp., and in a whole cell assay, it inhibited PARP activity with EC50 = 4 nM and inhibited proliferation of canc 5 mg
P-3019-48 EpiQuik DNA Demethylase Activity per Inhibition Assay Kit 48 assays
32-179 Interleukine 6 (IL-6) is a cytokine produced primarily by stimulated monocytes, fibroblasts and epithelial cells. It has a wide variety of biological functions through activating a cellsurface signali 0.1 mL
P-3008-48 EpiQuik DNA Demethylase Activity per Inhibition Assay Ultra Kit 48 assays
P-3086-48 Epigenase 5mC-Hydroxylase TET Activity per Inhibition Assay Kit Colorimetric 48 assays
P-4002-48 EpiQuik HDAC Activity per Inhibition Assay Kit Colorimetric 48 assays


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur