Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :30307971
Publication Date : //

Anti-cancer effects of 3,5-dimethylaminophenol in A549 lung cancer cells.


Exposure to 3,5-dimethylaminophenol (3,5-DMAP), the metabolite of the 3-5-dimethylaniline, was shown to cause high levels of oxidative stress in different cells. The aim of the present work was to observe whether this metabolite can lead to cytotoxicity, oxidative stress, DNA damage and cell cycle changes in non-small cell lung cancer A549 cells. 3,5-DMAP caused a dose-dependent increase in cytotoxicity, generation of superoxide (O2-.), inductions in the enzyme activities orchestrating cellular antioxidant balance, increases in lipid peroxidation as well as DNA damage. However, 3,5-DMAP showed significantly lower cytotoxicity towards human lung fibroblast (HLF) cells. 3,5-DMAP also led to molecular events, like inducing apoptotic markers (ie. p53, Bad, Bax and cytochrome c); decreasing anti-apoptotic proteins (Bcl-2) and alterations in cell cycle. Our findings indicate that the cytotoxicity caused by this particular alkylaniline metabolite led to initiation of caspase 3-mediated apoptosis. Furthermore, 3,5-DMAP attenuated carcinogenic properties like migration capacity of A549 cells and eventually inhibited growth of A549 cells in an in vivo mouse model. Tumor sections showed that 3,5-DMAP down-regulated c-Myc expression but up-regulated p53 and cytochrome c, all of which might result in tumor growth arrest. Co-treatment with N-acetylcysteine provided reductions in cytotoxicity and positively modulated genetic events induced by 3,5-DMAP in A549 cells. In conclusion, our findings demonstrate 3,5-DMAP may be a potential anti-cancer drug in cancer, due to its self redox cycling properties.

Authors : Lin Pei-Ying , Chang Yu-Jung , Chen Yu-Chen , Lin Chin-Hung , Erkekoglu Pinar , Chao Ming-Wei , Tseng Chia-Yi ,

Related products :

Catalog number Product name Quantity
Lung - LUD481 Lung cancer array, 16 cases covering all the common types of lung cancer paired with uninvolved lung tissues from the same patients. 2mm
Lung - LUC481 Lung cancer array, 16 cases covering all the common types of lung cancer paired with uninvolved lung tissues from the same patients. 2mm
Lung - LUC1502 Lung cancer array, non_overlapping with LUC1501, consists of duplicated 70 cases covering all the common types of lung cancer and 5 cases of normal and other non_malignant lung tissues. 1.1 mm
Lung - LUC1501 Lung cancer array consists of duplicated 70 cases covering all the common types of lung cancer and 5 cases of normal and other non_malignant lung tissues. 1.1 mm
Lung - LUC961 Lung cancer array consists of duplicated 36 cases covering all the common types of lung cancer and 12 cases of normal and other non_malignant lung tissues. 1.5mm
LUC1504 Lung cancer tissue array (small cell lung cancer), 75 cases, 150 samples (1.1mm), set 4 75 Cases
LC2085a High-density (188 cases_208 cores), multiple types of lung cancer (I-3 grade) tissue array with normal lung from autopsy and cancer adjacent tissue; ihc Anti-Cytokeratin (CK) confirmed
orb61244 Erlotinib Erlotinib hydrochloride is a drug used to treat non-small cell lung cancer, pancreatic cancer and several other types of cancer. For research use only. 100 mg
5-81020 Human lung smooth muscle cells (normal), male, 64, lung cancer vial
LC2085a High_density (188 cases_208 cores), multiple types of lung cancer (I_3 grade) tissue array with normal lung from autopsy and cancer adjacent tissue
LC2085b High-density (208 cores), multiple types of lung cancer (grade 1-3) tissue array with normal lung and cancer adjacent tissue, and staging info, 188 cases_208 cores (duplicate of LC2085a); ihc Anti-Cy
orb60893 Matrine((+)-Matrine) Matrine((+)-Matrine) is an alkaloid found in plants from the Sophora family. It has a variety of pharmacological effects, including anti-cancer effects, and action as a kappa opio 50 mg
CLC601 Colon cancer and lung cancer tissue array, including TNM and pathology grade, 60 cases_60 cores; ihc Anti-Actin confirmed
SLC601 Stomach cancer and lung cancer tissue array, including TNM and pathology grade, 60 cases_60 cores; ihc Anti-Actin confirmed
Lung - LUC962 Without overlaping with cancer cases in LUC961, LUC962 consists of further 36 cases in duplicates covering all the common types of lung cancer and 12 cases of normal and other non_malignant lung tis 1.5 mm
LC2085b High_density (208 cores), multiple types of lung cancer (grade 1_3) tissue array with normal lung and cancer adjacent tissue, and staging info, 188 cases_208 cores (duplicate of LC2085a)
CLC601 Colon cancer and lung cancer tissue array, including TNM and pathology grade, 60 cases_60 cores
SLC601 Stomach cancer and lung cancer tissue array, including TNM and pathology grade, 60 cases_60 cores
EIAAB11376 Deleted in lung and esophageal cancer protein 1,Deleted in lung cancer protein 1,DLC1,DLC-1,DLEC1,Homo sapiens,Human
9-46038 Human Cancer PrimaCell™15: Skin Cancer Cells, primary cell culture 5 x 100 ml
9-80038 Human Cancer PrimaCell™15: Skin Cancer Cells, primary cell culture 1 x 100 ml
2-96038 Human Cancer PrimaCell™15: Skin Cancer Cells, primary cell culture Kit
9-47038 Human Cancer PrimaCell™15: Skin Cancer Cells, primary cell culture set
4-28038 Human Cancer PrimaCell™15: Skin Cancer Cells, primary cell culture Kit
E14L0296 Sheep Lung cancer marker ELISA, Lung cancer marker


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur