Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :30320371
Publication Date : //

HLTF suppresses the migration and invasion of colorectal cancer cells via TGF‑β/SMAD signaling in vitro.


Helicase‑like transcription factor (HLTF) has been identified as a tumor suppressor gene. The hypermethylation of HTLF is frequently observed in various types of cancer, including colorectal cancer (CRC). However, the mechanisms through which HLTF suppresses CRC progression remain unclear. Thus, the aim of the present study was to explore the biological function of HLTF in CRC cells and the underlying mechanisms. CRC tissues and cells were used to detect the expression of HLTF. Wound‑healing and Transwell assays were performed to assess the motility of CRC cells. The results revealed that HLTF expression was significantly associated with the differentiation status, invasion depth, lymph node metastasis and distant metastasis. A low HLTF expression was significantly associated with a poor survival. Furthermore, HTLF knockdown or ectopic overexpression significantly promoted or suppressed the motility of CRC cells, respectively. With regard to the underlying molecular mechanisms, the protein expression of HTLF was upregulated when the CRC cells were stimulated with transforming growth factor (TGF)‑β, and HLTF upregulation induced an increase in SMAD4 and p‑SMAD2/3 expression and a decrease in levels of the TGF‑β/SMAD pathway downstream genes, Vimentin and zinc finger e‑box binding homeobox 1 (ZEB1). On the whole, the findings of this study suggest that HLTF is negatively associated with the progression of CRC, and its overexpression suppresses the migration and invasion of CRC cells by targeting the TGF‑β/SMAD pathway.

Authors : Liu Li , Liu Huan , Zhou Yangying , He Jiaofeng , Liu Qiong , Wang Jian , Zeng Manting , Yuan Dan , Tan Fengbo , Zhou Yuan , Pei Haiping , Zhu Hong ,

Related products :

Catalog number Product name Quantity
28-405 CREBZF strongly activates transcription when bound to HCFC1. CREBZF suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. It also suppresses the HCFC1 0.1 mg
YM8096 CA19_9, SW1116 colorectal cancer cells, Mab anti_Human; Clone 1116_NS_19_9 1 ml.
9-47029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture set
9-80029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture 1 x 100 ml
9-46029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture 5 x 100 ml
2-96029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture Kit
4-28029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture Kit
3471-096-K AIM Kit Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit 96 tests
3471-096-K Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit 96 tests
3471-096-K CultrexIn Vitro Angiogenesis Assay Endothelial Cell Invasion Kit 96 tests
EIAAB24761 D4Wsu114e,IIp45,Iip45,Invasion-inhibitory protein 45,Migration and invasion-inhibitory protein,Miip,Mouse,Mus musculus
27-473 SMAD1 belongs to the SMAD family. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1 mediates the signals of the bone morphogenetic pro 0.05 mg
28-921 SMAD1 belongs to the SMAD family. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1 mediates the signals of the bone morphogenetic pro 0.1 mg
28-922 SMAD1 belongs to the SMAD family. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1 mediates the signals of the bone morphogenetic pro 0.1 mg
EIAAB24762 Homo sapiens,Human,IGFBP2-binding protein,IIp45,IIP45,Invasion-inhibitory protein 45,Migration and invasion-inhibitory protein,MIIP
EIAAB24760 IIp45,Iip45,Invasion-inhibitory protein 45,Migration and invasion-inhibitory protein,Miip,Rat,Rattus norvegicus
ICTS-SER Cancer samples: Colorectal Cancer Serum-Adenocarcinoma Each
Tissues-123-COLORECTAL CANCER Human Tissues - COLORECTAL CANCER 1unit
E0535h Bovine ELISA Kit FOR Migration and invasion enhancer 1 96T
E4142h Human Migration And Invasion Inhibitory Protein EL 96T
3455-024-01 24 Insert Cell Invasion per Migration Plates 2 plates
ODFP2_HUMAN Bovine ELISA Kit FOR Migration and invasion enhancer 1 96T
20-372-60280 SMAD family member 1 (SMAD1). transcript variant 1 - Mouse monoclonal anti-human SMAD1 antibody; SMAD 1; Mothers against DPP homolog 1; Mad-related protein 1; Transforming growth factor-beta-signaling 0.1 mg
MINA MIIP Gene migration and invasion inhibitory protein
MIIP_MOUSE Mouse ELISA Kit FOR Migration and invasion-inhibitory protein 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur