Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :7535774
Publication Date : //

HER4 expression correlates with cytotoxicity directed by a heregulin-toxin fusion protein.

We have constructed, expressed, and purified a fusion protein, HAR-TX beta 2, consisting of heregulin-beta 2 fused to a binding-defective form of Pseudomonas exotoxin A, PE40. The fusion protein was found to induce receptor tyrosine phosphorylation in CEM cells transfected with HER4 alone or in combination with HER2 but not in cells transfected with HER2 or HER1 alone. The phosphorylation of receptor tyrosines was both dose-dependent and saturable in amounts similar to those shown to be active for native heregulin. HAR-TX beta 2 was specifically cytotoxic toward a variety of carcinoma cell lines in the ng/ml range. However, some tumor cell lines were found to be insensitive to the cytotoxic action of the fusion protein even at > 2 micrograms/ml. Relative amounts of HER4, HER3, and HER2 were determined on seven cell lines sensitive and four cell lines insensitive to HAR-TX beta 2. All lines that express HER4 were killed by HAR-TX beta 2, while none lacking HER4 were affected. HAR-TX beta 2 was able to bind to and signal via tyrosine phosphorylation in cell lines that co-express HER2 and HER3 in the absence of HER4 without inducing cytotoxicity. Thus HAR-TX beta 2 may prove to be a useful reagent for the targeting and elimination of HER4-positive tumor cells.

Authors : Siegall C B , Bacus S S , Cohen B D , Plowman G D , Mixan B , Chace D , Chin D M , Goetze A , Green J M , Hellström I ,

Related products :

Catalog number Product name Quantity
28-078 NSEP1 is a cell cycle stage-specific transcription factor important for cell proliferation. NSEP1 encodes a protein that correlates with P-glycoprotein expression in human breast carcinoma. 0.05 mg
EIAAB27950 Erg1,NEM-sensitive fusion protein,N-ethylmaleimide-sensitive fusion protein,Nsf,Rat,Rattus norvegicus,Vesicle-fusing ATPase,Vesicular-fusion protein NSF
EIAAB27951 Homo sapiens,Human,NEM-sensitive fusion protein,N-ethylmaleimide-sensitive fusion protein,NSF,Vesicle-fusing ATPase,Vesicular-fusion protein NSF
EIAAB27952 Mouse,Mus musculus,NEM-sensitive fusion protein,N-ethylmaleimide-sensitive fusion protein,Nsf,Protein SKD2,Skd2,Suppressor of K(+) transport growth defect 2,Vesicle-fusing ATPase,Vesicular-fusion prot
KA1435 Protein Expression Kit I (GST-Fusion Protein) 1 Kit
KA1436 Protein Expression Kit I (His-Fusion Protein) 1 Kit
KA1438 Protein Expression Kit II (GST-Fusion Protein) 1 Kit
KA1439 Protein Expression Kit II (His-Fusion Protein) 1 Kit
orb80263 Rat Apoliprotein J protein The Clusterin Rat was constructed as recombinant protein with N-terminal fusion of T7-Tag (16AA) and C-terminal fusion of His-Tag (9AA). The Clusterin Rat His-Tagged Fusion 2
MD-14-0952 Mouse Anti-Repsiratory Synctycial Virus (RSV) Fusion Protein (46kDa & 22kDa S-S linked)RSV Fusion Protein 1 mg
EIAAB13165 ERBB4,HER4,Homo sapiens,Human,p180erbB4,Proto-oncogene-like protein c-ErbB-4,Receptor tyrosine-protein kinase erbB-4,Tyrosine kinase-type cell surface receptor HER4
EIAAB45257 Homo sapiens,Human,UB fusion protein 1,Ubiquitin fusion degradation protein 1 homolog,UFD1L
EIAAB45256 Mouse,Mus musculus,UB fusion protein 1,Ubiquitin fusion degradation protein 1 homolog,Ufd1l
EIAAB45258 Rat,Rattus norvegicus,UB fusion protein 1,Ubiquitin fusion degradation protein 1 homolog,Ufd1l
CQLD-100 QuantiChrom™ LDH Cytotoxicity Assay Kit, Quantitative determination of cytotoxicity based on cellular lactate dehydrogenase release into cell culture medium by colorimetric (565nm) method. Procedure 100tests
26-899 FAU is a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationall 0.05 mg
EIAAB35767 AC40,DNA-directed RNA polymerase I subunit C,DNA-directed RNA polymerases I and III 40 kDa polypeptide,DNA-directed RNA polymerases I and III subunit RPAC1,Homo sapiens,Human,POLR1C,POLR1E,RNA polymer
orb81333 Human S100 Calcium Binding Protein A3 protein The S100A3 Human Recombinant is manufactured with N-terminal fusion of 10 amino acid His Tag. The S100A3 His -Tagged Fusion Protein is 13kDa protein conta 2
orb81337 Human S100 Calcium Binding Protein A7A protein The S100A7A Human Recombinant is manufactured with N-terminal fusion of amino acids His Tag. The S100A7A His -Tagged Fusion Protein is 12.3kDa protein co 2
EIAAB35755 DNA-directed RNA polymerase II subunit F,DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide,DNA-directed RNA polymerases I, II, and III subunit RPABC2,Homo sapiens,Human,POLR2F,POLRF,RNA
EIAAB35759 DNA-directed RNA polymerase II subunit H,DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide,DNA-directed RNA polymerases I, II, and III subunit RPABC3,Homo sapiens,hRPB8,Human,POLR2H,RNA
orb81315 Human Regenerating Islet-Derived Alpha protein The Recombinant Human REG3A protein is produced with N-terminal fusion of His Tag. The REG3A His -Tagged Fusion Protein is an 18.4 kDa protein containing 2
orb80245 Mouse Adiponectin His tag protein The Acrp30 Mouse is created as recombinant protein with N-terminal fusion of His Tag.The Adiponectin His-Tagged Fusion Protein, produced in E.coli, is 27 kDa protein 5
orb80373 Human gm-csf_IL-3 Fusion Protein (PIXY321) protein GMCSF_IL3 fusion protein Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain having Mw of 30kDa.PIXY321 is purified 2
EIAAB38219 CNSA1,EEN,EEN fusion partner of MLL,Endophilin-2,Endophilin-A2,Extra eleven-nineteen leukemia fusion gene protein,Homo sapiens,Human,SH3 domain protein 2B,SH3 domain-containing GRB2-like protein 1,SH3

https://antibody-antibodies.com/ | https://gentaur.com/ | https://gen-script.com/ | https://diagenox.com/ | https://clonagen.com/ | http://gentaursearch.com/ | http://gentaurpub.com/ | https://gentaur-online.com/ | http://anti-anti-pdf.com/ | http://gentaur-worldwide.com/


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur

Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur