Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :8836445
Publication Date : //

Unusually stable NAD-specific glutamate dehydrogenase from the alkaliphile Amphibacillus xylanus.


Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase (NAD-GDH; EC 1.4.1.3) from Amphibacillus xylanus DSM 6626 was enriched 100-fold to homogeneity. The molecular mass was determined by native polyacrylamide electrophoresis and by gel filtration to be 260 kDa (+/- 25 kDa); the enzyme was composed of identical subunits of 45 (+/- 5) kDa, indicating that the native enzyme has a hexameric structure. NAD-GDH was highly specific for the coenzyme NAD(H) and catalyzed both the formation and the oxidation of glutamate. Apparent Km-values of 56 mM glutamate, 0.35 mM NAD (oxidative deamination) and 6.7 mM 2-oxoglutaric acid, 42 mM NH4Cl and 0.036 mM NADH (reductive amination) were measured. The enzyme was unusually resistant towards variation of pH, chaotropic agents, organic solvents, and was stable at elevated temperature, retaining 50% activity after 120 min incubation at 85 degrees C.

Authors : Jahns T ,

Related products :

Catalog number Product name Quantity
26-934 Glutamate dehydrogenase (EC 1.4.1.3) catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate using NAD and_or NADP as cofactors.Glutamate dehydrogenase (EC 1.4.1.3) catalyze 0.05 mg
18-783-77679 RABBIT ANTI BOVINE GLUTAMATE DEHYDROGENASE - GLUTAMATE DEHYDROGENASE; Polyclonal 1 ml
'AP23724PU-N Glutamate dehydrogenase 1 (+Glutamate dehydrogenase 2) antibody Ab host: Goat 0.1 mg
'AP23724PU-N Glutamate dehydrogenase 1 (+Glutamate dehydrogenase 2) antibody Host Goat 0.1 mg
AP23724PU-N Glutamate dehydrogenase 1 (+Glutamate dehydrogenase 2) Goat antibody Ab Aff - Purified 0.1 mg
LF-PA41789 anti-Glutamate Dehydrogenase 1, Rabbit polyclonal to Glutamate Dehydrogenase 1, Isotype IgG, Host Rabbit 50 ug
18-783-77681 RABBIT ANTI HUMAN GLUTAMATE DECARBOXYLASE - GLUTAMATE DEHYDROGENASE; EC 4.1.1.15; Glutamate decarboxylase 67 kDa isoform; GAD-67; 67 kDa glutamic acid decarboxylase Polyclonal 0.05 ml
SCH-4670-5488 RABBIT ANTI BOVINE GLUTAMATE DEHYDROGENASE, Product Type Polyclonal Antibody, Specificity GLUTAMATE DEHYDROGENASE, Target Species Bovine, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, A 1 ml
4670-5488 RABBIT ANTI BOVINE GLUTAMATE DEHYDROGENASE, Product Type Polyclonal Antibody, Specificity GLUTAMATE DEHYDROGENASE, Target Species Bovine, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, A 1 ml
9001-46-1 Glutamate Dehydrogenase Glutamate Dehydrogen 1g
18-003-43731 Isocitrate dehydrogenase [NAD] subunit alpha. mitochondrial - EC 1.1.1.41; Isocitric dehydrogenase; NAD(+)-specific ICDH Polyclonal 0.1 mg Protein A
Y46485003 Glutamate Dehydrogenase 150U
R1086HRP Glutamate dehydrogenase 1 20 mg
Y46486003 Glutamate Dehydrogenase 600U
AP20095AF-N Glutamate dehydrogenase 1 10 mg
Y46485003 Glutamate Dehydrogenase 150U
527 GLUTAMATE DEHYDROGENASE, SERUM 1
R1086 Glutamate dehydrogenase 1 2 ml
R1086B Glutamate dehydrogenase 1 10 mg
AP08859PU-N Glutamate dehydrogenase 1 50 µl
GTX41142 Glutamate dehydrogenase 1 1 mg
AP20095BT-N Glutamate dehydrogenase 1 1 ml
NB600-853 Glutamate dehydrogenase 1 0.2 ml
ARP54827_P050 Glutamate dehydrogenase 2 50 µg
NB600-854 Glutamate dehydrogenase 1 2 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur