Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :8885241
Publication Date : //

Expression of alternatively spliced isoforms of the parathyroid hormone (PTH)/PTH-related peptide receptor messenger RNA in human kidney and bone cells.


Using a PCR-based strategy, two variants of the PTH/PTH-related peptide (PTH-rp) receptor mRNA were identified in human kidney, SaOS-2 human osteoblast cells, and rat bone that are produced by alternative splicing of exons coding for the N-terminal portion of the receptor. In the S-N3-E2 isoform, the exon coding the signal peptide (S) is spliced to an alternative 3'-acceptor site, producing a product respecting the reading frame, but in which the E1 exon is replaced by 12 amino acids derived from the N3 intron. In the S-E2 isoform, in which the E1 exon is deleted by cassette exclusion, the reading frame is changed, but a truncated receptor may be produced by reinitiation of translation at an overlapping stop/start codon. After transfection of COS and Chinese hamster ovary cells with the originally described S-E1-E2 isoform and the two splice variants, active transcription of PTH/PTH-rp receptor mRNA was detected by RT-PCR in all cases. Cell lines transfected with the S-E1-E2 and S-N3-E2 isoforms displayed a 15- to 25-fold and 2- to 3-fold increase, respectively, in cAMP content after stimulation with 2.4 x 10(-7) M human PTH(1-34), whereas cells transfected with the S-E2 isoform did not respond. PTH elicited an increase in intracellular calcium only in cells transfected with the S-E1-E2 isoform. Studies evaluating the surface expression of receptors using anti-human PTH/PTH-rp receptor antibodies and the ability of transfected cells to bind [125I]PTH-rp indicated that the low or absent responses to PTH stimulation resulted, at least in part, from low surface expression of the S-N3-E2 and S-E2 isoforms. These studies support the conclusion that exon E1 is extremely important in promoting surface expression of the PTH/PTH-rp receptor but indicate that isoforms lacking this exon can retain the ability to recognize PTH. The possible intracellular expression of these splice variants, which account for 15-20% of total PTH/PTH-rp receptor mRNA, needs to be evaluated.

Authors : Jobert A S , Fernandes I , Turner G , Coureau C , Prie D , Nissenson R A , Friedlander G , Silve C ,

Related products :

Catalog number Product name Quantity
EIAAB32991 Homo sapiens,Human,Parathyroid hormone 1 receptor,Parathyroid hormone_parathyroid hormone-related peptide receptor,PTH_PTHr receptor,PTH_PTHrP type I receptor,PTH1 receptor,PTH1R,PTHR,PTHR1
EIAAB32990 Parathyroid hormone 1 receptor,Parathyroid hormone_parathyroid hormone-related peptide receptor,PTH_PTHr receptor,PTH_PTHrP type I receptor,PTH1 receptor,Pth1r,Pthr,Pthr1,Rat,Rattus norvegicus
EIAAB32987 Mouse,Mus musculus,Parathyroid hormone 1 receptor,Parathyroid hormone_parathyroid hormone-related peptide receptor,PTH_PTHr receptor,PTH_PTHrP type I receptor,PTH1 receptor,Pth1r,Pthr,Pthr1
EIAAB32989 Bos taurus,Bovine,Parathyroid hormone 1 receptor,Parathyroid hormone_parathyroid hormone-related peptide receptor,PTH_PTHr receptor,PTH_PTHrP type I receptor,PTH1 receptor,PTH1R,PTHR1
EIAAB32988 Parathyroid hormone 1 receptor,Parathyroid hormone_parathyroid hormone-related peptide receptor,Pig,PTH_PTHr receptor,PTH_PTHrP type I receptor,PTH1 receptor,PTH1R,PTHR,PTHR1,Sus scrofa
PTH1R_MOUSE ELISA Kit FOR Parathyroid hormone per parathyroid hormone-related peptide receptor; organism: Mouse; gene name: Pth1r 96T
PTH1R_RAT ELISA Kit FOR Parathyroid hormone per parathyroid hormone-related peptide receptor; organism: Rat; gene name: Pth1r 96T
GWB-DA2092 Anti- Parathyroid hormone parathyroid hormone-related peptide receptor Antibody
PTH1R_BOVIN Bovine ELISA Kit FOR Parathyroid hormone per parathyroid hormone-related peptide receptor 96T
7170-9600 MOUSE ANTI HUMAN PARATHYROID HORMONE RELATED PEPTIDE, Product Type Monoclonal Antibody, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species Human, Host Mouse, Format Purified, Isotype 0.2 mg
SCH-7170-9600 MOUSE ANTI HUMAN PARATHYROID HORMONE RELATED PEPTIDE, Product Type Monoclonal Antibody, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species Human, Host Mouse, Format Purified, Isotype 0.2 mg
7170-9208 RABBIT ANTI HUMAN PARATHYROID HORMONE RELATED PEPTIDE, Product Type Polyclonal Antibody, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species Human, Host Rabbit, Format Purified, Isoty 0.1 ml
SCH-7170-9611 MOUSE ANTI HUMAN PARATHYROID HORMONE RELATED PEPTIDE, Product Type Monoclonal Antibody, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species Human, Host Mouse, Format Purified, Isotype 0.2 mg
7170-9611 MOUSE ANTI HUMAN PARATHYROID HORMONE RELATED PEPTIDE, Product Type Monoclonal Antibody, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species Human, Host Mouse, Format Purified, Isotype 0.2 mg
SCH-7170-9208 RABBIT ANTI HUMAN PARATHYROID HORMONE RELATED PEPTIDE, Product Type Polyclonal Antibody, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species Human, Host Rabbit, Format Purified, Isoty 0.1 ml
SCH-7170-9355 SYNTHETIC HUMAN PARATHYROID HORMONE RELATED PEPTIDE, Product Type Purified Protein, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species Human, Host N_A, Format Purified, Isotypes , A 0.1 mg
7170-9355 SYNTHETIC HUMAN PARATHYROID HORMONE RELATED PEPTIDE, Product Type Purified Protein, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species Human, Host N_A, Format Purified, Isotypes , A 0.1 mg
A 4241AG.1 Parathyroid Hormone related Peptide (PTHrP) antigen: human Parathyroid Hormone related Peptide (aa 107-111) (Osteostatin) 100
A 4241AG.2 Parathyroid Hormone related Peptide (PTHrP) antigen: human Parathyroid Hormone related Peptide (aa 107-111) (Osteostatin) 1 mg
A 1118AG.1 Parathyroid Hormone related Peptide (PTHrP) antigen: human Parathyroid Hormone related Peptide (aa 53-86) 100
A 7881AG.1 Parathyroid Hormone related Peptide (PTHrP) antigen: human Parathyroid Hormone related Peptide (aa 1-34) 100
A 1118AG.2 Parathyroid Hormone related Peptide (PTHrP) antigen: human Parathyroid Hormone related Peptide (aa 53-86) 1 mg
A 7881AG.2 Parathyroid Hormone related Peptide (PTHrP) antigen: human Parathyroid Hormone related Peptide (aa 1-34) 1 mg
MCA3389Z MOUSE ANTI HUMAN PARATHYROID HORMONE RELATED PEPTIDE Azide Free, Product Type Monoclonal Antibody, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species Human, Host Mouse, Format Azide 0.1 mg
0100-0670 RABBIT ANTI PARATHYROID HORMONE RELATED PEPTIDE, Product Type Polyclonal Antibody, Specificity PARATHYROID HORMONE RELATED PEPTIDE, Target Species , Host Rabbit, Format Purified, Isotypes Polycl 0.2 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur