Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :10493790
Publication Date : //

Expression, purification, and crystal structure determination of recombinant human epidermal-type fatty acid binding protein.


We describe the crystal structure of human epidermal-type fatty acid binding protein (E-FABP) that was recently found to be highly upregulated in human psoriatic keratinocytes. To characterize E-FABP with respect to ligand-binding properties and tertiary structure, we cloned the respective cDNA, overexpressed the protein in Escherichia coli and purified it to homogeneity by a combination of ion-exchange and size-exclusion chromatographic steps with a yield of 30 mg/L broth. The purified protein revealed a 5-fold higher affinity for stearic acid than for oleic and arachidonic acids. The crystal structure of recombinant human E-FABP was determined to 2.05 A and refined to an R(factor) of 20.7%. The initial residual electron density maps clearly showed the presence of a ligand, which was identified as endogenous bacterial fatty acid. Within a central cavity of 252 A(3), this ligand is bound in a U-shaped conformation, its carboxyl group interacting with tyrosine 131 and arginines 129 and 109, the latter via an ordered water molecule. The E-FABP crystal structure is unique in the FABP family because of the presence of a disulfide bridge between cysteines 120 and 127 that may be physiologically as well as pathophysiologically relevant. Cysteines 67 and 87 are also in close vicinity but in contrast do not form a disulfide bridge. We postulate that this protein belongs to a particular FABP subfamily whose members share common structural as well as functional features.

Authors : Hohoff C , Börchers T , Rüstow B , Spener F , van Tilbeurgh H ,

Related products :

Catalog number Product name Quantity
E0344h ELISA E-FABP,Epidermal-type fatty acid-binding protein,FABP5,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal,Homo sapiens,Human,PA-FABP,Psoriasis-associated fatty acid-binding prote 96T
U0344h CLIA E-FABP,Epidermal-type fatty acid-binding protein,FABP5,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal,Homo sapiens,Human,PA-FABP,Psoriasis-associated fatty acid-binding protei 96T
E0344h ELISA kit E-FABP,Epidermal-type fatty acid-binding protein,FABP5,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal,Homo sapiens,Human,PA-FABP,Psoriasis-associated fatty acid-binding 96T
E0344m ELISA E-FABP,Epidermal-type fatty acid-binding protein,Fabp5,Fabpe,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal,Keratinocyte lipid-binding protein,Klbp,Mal1,Mouse,Mus musculus,PA 96T
U0344m CLIA E-FABP,Epidermal-type fatty acid-binding protein,Fabp5,Fabpe,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal,Keratinocyte lipid-binding protein,Klbp,Mal1,Mouse,Mus musculus,PA- 96T
E0344m ELISA kit E-FABP,Epidermal-type fatty acid-binding protein,Fabp5,Fabpe,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal,Keratinocyte lipid-binding protein,Klbp,Mal1,Mouse,Mus muscul 96T
U0344b CLIA Bos taurus,Bovine,Differentiation-associated lipid-binding protein LP2,E-FABP,Epidermal-type fatty acid-binding protein,FABP5,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal 96T
E0344b ELISA Bos taurus,Bovine,Differentiation-associated lipid-binding protein LP2,E-FABP,Epidermal-type fatty acid-binding protein,FABP5,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal 96T
E0344r ELISA C-FABP,Cutaneous fatty acid-binding protein,DA11,E-FABP,Epidermal-type fatty acid-binding protein,Fabp5,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal,Rat,Rattus norvegicus 96T
U0344r CLIA C-FABP,Cutaneous fatty acid-binding protein,DA11,E-FABP,Epidermal-type fatty acid-binding protein,Fabp5,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal,Rat,Rattus norvegicus 96T
E0344r ELISA kit C-FABP,Cutaneous fatty acid-binding protein,DA11,E-FABP,Epidermal-type fatty acid-binding protein,Fabp5,Fatty acid-binding protein 5,Fatty acid-binding protein, epidermal,Rat,Rattus norvegi 96T
E0344b ELISA kit Bos taurus,Bovine,Differentiation-associated lipid-binding protein LP2,E-FABP,Epidermal-type fatty acid-binding protein,FABP5,Fatty acid-binding protein 5,Fatty acid-binding protein, epider 96T
OETPRO417 Epidermal Fatty Acid Binding Protein Human Recombinant 10
OETPRO417 Epidermal Fatty Acid Binding Protein Human Recombinant 2
ACLPRO417B Fatty Acid Binding Protein (FABP_5), Human Epidermal, recombinant 10 µg.
ACLPRO417 Fatty Acid Binding Protein (FABP_5), Human Epidermal, recombinant 2 µg.
U0184h CLIA Adipocyte lipid-binding protein,Adipocyte-type fatty acid-binding protein,AFABP,A-FABP,ALBP,FABP4,Fatty acid-binding protein 4,Fatty acid-binding protein, adipocyte,Homo sapiens,Human 96T
E0184h ELISA Adipocyte lipid-binding protein,Adipocyte-type fatty acid-binding protein,AFABP,A-FABP,ALBP,FABP4,Fatty acid-binding protein 4,Fatty acid-binding protein, adipocyte,Homo sapiens,Human 96T
E0184h ELISA kit Adipocyte lipid-binding protein,Adipocyte-type fatty acid-binding protein,AFABP,A-FABP,ALBP,FABP4,Fatty acid-binding protein 4,Fatty acid-binding protein, adipocyte,Homo sapiens,Human 96T
U0559h CLIA FABP2,FABPI,Fatty acid-binding protein 2,Fatty acid-binding protein, intestinal,Homo sapiens,Human,I-FABP,Intestinal-type fatty acid-binding protein 96T
E2204h ELISA FABP1,FABPL,Fatty acid-binding protein 1,Fatty acid-binding protein, liver,Homo sapiens,Human,L-FABP,Liver-type fatty acid-binding protein 96T
U2204h CLIA kit FABP1,FABPL,Fatty acid-binding protein 1,Fatty acid-binding protein, liver,Homo sapiens,Human,L-FABP,Liver-type fatty acid-binding protein 96T
E0559h ELISA kit FABP2,FABPI,Fatty acid-binding protein 2,Fatty acid-binding protein, intestinal,Homo sapiens,Human,I-FABP,Intestinal-type fatty acid-binding protein 96T
E2204h ELISA kit FABP1,FABPL,Fatty acid-binding protein 1,Fatty acid-binding protein, liver,Homo sapiens,Human,L-FABP,Liver-type fatty acid-binding protein 96T
U2204h CLIA FABP1,FABPL,Fatty acid-binding protein 1,Fatty acid-binding protein, liver,Homo sapiens,Human,L-FABP,Liver-type fatty acid-binding protein 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur