Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :10954572
Publication Date : //

Influenza virus assembly: effect of influenza virus glycoproteins on the membrane association of M1 protein.


Influenza virus matrix protein (M1), a critical protein required for virus assembly and budding, is presumed to interact with viral glycoproteins on the outer side and viral ribonucleoprotein on the inner side. However, because of the inherent membrane-binding ability of M1 protein, it has been difficult to demonstrate the specific interaction of M1 protein with hemagglutinin (HA) or neuraminidase (NA), the influenza virus envelope glycoproteins. Using Triton X-100 (TX-100) detergent treatment of membrane fractions and floatation in sucrose gradients, we observed that the membrane-bound M1 protein expressed alone or coexpressed with heterologous Sendai virus F was totally TX-100 soluble but the membrane-bound M1 protein expressed in the presence of HA and NA was predominantly detergent resistant and floated to the top of the density gradient. Furthermore, both the cytoplasmic tail and the transmembrane domain of HA facilitated binding of M1 to detergent-resistant membranes. Analysis of the membrane association of M1 in the early and late phases of the influenza virus infectious cycle revealed that the interaction of M1 with mature glycoproteins which associated with the detergent-resistant lipid rafts was responsible for the detergent resistance of membrane-bound M1. Immunofluorescence analysis by confocal microscopy also demonstrated that, in influenza virus-infected cells, a fraction of M1 protein colocalized with HA and associated with the HA in transit to the plasma membrane via the exocytic pathway. Similar results for colocalization were obtained when M1 and HA were coexpressed and HA transport was blocked by monensin treatment. These studies indicate that both HA and NA interact with influenza virus M1 and that HA associates with M1 via its cytoplasmic tail and transmembrane domain.

Authors : Ali A , Avalos R T , Ponimaskin E , Nayak D P ,

Related products :

Catalog number Product name Quantity
orb81796 Influenza Virus H1N1 New Caledonia 20_99 IV 116 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain A_ New Caledonia_20_99 IVR 116. The Influenza Virus was 10
orb81790 Influenza Virus Tokio 53_99 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain B_Tokio_53_99. The Influenza Virus was purified by Ultracentrifugation with 10
orb81789 Influenza Virus Qingdao 102_91 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain B_Qingdao_102_91. The Influenza Virus was purified by Ultracentrifugatio 10
orb81791 Influenza Virus Victoria 504_00 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain B_Victoria_504_00. The Influenza Virus was purified by Ultracentrifugat 10
orb81793 Influenza Virus Florida 07_04 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain B_Florida_07_04. The Influenza Virus was purified by Ultracentrifugation 10
orb81792 Influenza Virus Malaysia 2506_04 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain B_Malaysia_2506_04. The Influenza Virus was purified by Ultracentrifug 10
orb81794 Influenza Virus Florida 04_06 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain B_Florida_04_06. The Influenza Virus was purified by Ultracentrifugation 10
orb81802 Influenza Virus H3N2 Wisconsin 67_05 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain A_Wisconsin_67_05. The Influenza Virus was purified by Ultracentri 10
orb81799 Influenza Virus H3N2 Shangdong 9_93 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain A_Shandong_9_93. The Influenza Virus was purified by Ultracentrifug 10
orb81803 Influenza Virus H3N2 Brisbane 10_07 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain A_Brisbane_10_07. The Influenza Virus was purified by Ultracentrifu 10
orb81801 Influenza Virus H3N2 Panama 2007_99 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain A_Panama_2007_99. The Influenza Virus was purified by Ultracentrifu 10
orb81797 Influenza Virus H1N1 Taiwan 1_86 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain A_Taiwan_1_86. The Influenza Virus was purified by Ultracentrifugation 10
orb81795 Influenza Virus H1N1 Beijing 262_95 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain A_Beijing_262_95. The Influenza Virus was purified by Ultracentrifu 10
orb81800 Influenza Virus H3N2 Kiev 301_94 like _Johannesburg 33_94 protein Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza virus, strain A_Kiev_301_94 like _Johannesburg_33_94. The I 10
H5N11-C Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A_chicken_India_NIV33487_2006) (17-531aa) protein control for Western Species Reactivity: Influenza Virus 100 ul Product tipe: Pure protein
H11N2-01-C Recombinant Purified Hemagglutinin Influenza A Virus (H11N2; A_duck_Yangzhou_906_2002) protein control for Western Species Reactivity: Influenza Virus 100 ul Product tipe: Pure protein
H5N11-S Anti-Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A_chicken_India_NIV33487_2006) (17-531aa) protein antiserum Species Reactivity: Influenza Virus 100 ul Product tipe: Antbodies
iha-031 Recombinant Hemagglutinin-Influenza A Virus H1N1 Solomon Island 03_2006 VIRAL ANTIGENS- INFLUENZA VIRUS 2
iha-031 Recombinant Hemagglutinin-Influenza A Virus H1N1 Solomon Island 03_2006 VIRAL ANTIGENS- INFLUENZA VIRUS 10
iha-031 Recombinant Hemagglutinin-Influenza A Virus H1N1 Solomon Island 03_2006 VIRAL ANTIGENS- INFLUENZA VIRUS 100
iha-013 Recombinant Hemagglutinin-Influenza A Virus H9N2 Hong Kong 1073_99 VIRAL ANTIGENS- INFLUENZA VIRUS 10
iha-013 Recombinant Hemagglutinin-Influenza A Virus H9N2 Hong Kong 1073_99 VIRAL ANTIGENS- INFLUENZA VIRUS 2
iha-013 Recombinant Hemagglutinin-Influenza A Virus H9N2 Hong Kong 1073_99 VIRAL ANTIGENS- INFLUENZA VIRUS 100
iha-009 Recombinant Hemagglutinin-Influenza A Virus H7N7 Netherlands 219_03 VIRAL ANTIGENS- INFLUENZA VIRUS 10
iha-014 Recombinant Hemagglutinin-Influenza A Virus H1N1 California 04_2009 VIRAL ANTIGENS- INFLUENZA VIRUS 2


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur