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Pubmed ID :11706190
Publication Date : //

A novel phospholipase D of Arabidopsis that is activated by oleic acid and associated with the plasma membrane.


Oleate-dependent phospholipase D (PLD; EC 3.1.4.4) has been reported in animal systems, but its molecular nature is unkown. Multiple PLDs have been characterized in plants, but none of the previously cloned PLDs exhibits the oleate-activated activity. Here, we describe the biochemical and molecular identification and characterization of an oleate-activated PLD in Arabidopsis. This PLD, designated PLDdelta, was associated tightly with the plasma membrane, and its level of expression was higher in old leaves, stems, flowers, and roots than in young leaves and siliques. A cDNA encoding the oleate-activated PLD was identified, and catalytically active PLDdelta was expressed from its cDNA in Escherichia coli. PLDdelta was activated by free oleic acid in a dose-dependent manner, with the optimal concentration being 0.5 mM. Other unsaturated fatty acids, linoleic and linolenic acids, were less effective than oleic acid, whereas the saturated fatty acids, stearic and palmitic acids, were totally ineffective. Phosphatidylinositol 4,5-bisphosphate stimulated PLDdelta to a lesser extent than oleate. Mutation at arginine (Arg)-611 led to a differential loss of the phosphatidylinositol 4,5-bisphosphate-stimulated activity of PLDdelta, indicating that separate sites mediate the oleate regulation of PLDdelta. Oleate stimulated PLDdelta's binding to phosphatidylcholine. Mutation at Arg-399 resulted in a decrease in oleate binding by PLDdelta and a loss of PLDdelta activity. However, this mutation bound similar levels of phosphatidylcholine as wild type, suggesting that Arg-399 is not required for PC binding. These results provide the molecular information on oleate-activated PLD and also suggest a mechanism for the oleate stimulation of this enzyme.

Authors : Wang C , Wang X ,

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Catalog number Product name Quantity
AS10 931 Antibody: AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence Q96262, Host: rabbit, polyclonal, 100
EIAAB31407 Oryctolagus cuniculus,Phospholipase AdRab-B,Phospholipase B,Phospholipase B_lipase,Phospholipase B1, membrane-associated,PLB_LIP,PLB1,Rabbit
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49553-76-6 oleic acid, monoester with oxybis(propan oleic acid, monoester w 1g
94139-24-9 oleic acid, diamide derivative with N-(2-a oleic acid, diamide deri 1g
AS12 2107 Antibody: MPK4 | Mitogen-activated protein kinase 4, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thalianaMPK4, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thal 200
AS10 931 rabbit polyclonal AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1 100
EIAAB31405 Homo sapiens,hPLB,Human,Phospholipase B,Phospholipase B_lipase,Phospholipase B1, membrane-associated,PLB,PLB_LIP,PLB1
EIAAB31404 Phlpb,Phospholipase B,Phospholipase B_lipase,Phospholipase B1, membrane-associated,PLB_LIP,Plb1,Rat,Rattus norvegicus
EIAAB31406 Mouse,Mus musculus,Phospholipase B,Phospholipase B_lipase,Phospholipase B1, membrane-associated,Plb,PLB_LIP,Plb1
28060-90-4 Oleic acid, ester with hydroxypropanediyl Oleic acid, ester with hy 1g
68310-76-9 oleic acid, compound with 2-(heptadec-8 oleic acid, compound w 1g
68228-04-6 oleic acid, compound with 2-(heptadecen oleic acid, compound w 1g
89560-81-6 oleic acid, compound with tributylamine ( oleic acid, compound w 1g
79665-93-3 Oleic acid, monoester with decaglycerol Oleic acid, monoester 1g
85614-40-0 oleic acid, monoester with (2-hydroxyeth oleic acid, monoester w 1g
94042-67-8 oleic acid, compound with octadecylamin oleic acid, compound w 1g
22256-71-9 oleic acid, compound with dicyclohexyla oleic acid, compound w 1g
68140-41-0 oleic acid, compound with 2-amino-2-me oleic acid, compound w 1g
68516-28-9 oleic acid, compound with 2-ethylhexyla oleic acid, compound w 1g
68052-47-1 oleic acid, compound with 2-(heptadec-8 oleic acid, compound w 1g
68133-62-0 oleic acid, compound with 2-(ethylamino) oleic acid, compound w 1g
67633-85-6 oleic acid, compound with 1,4-diazabicyc oleic acid, compound w 1g
68258-69-5 oleic acid, compound with (o-hydroxyben oleic acid, compound w 1g
52584-01-7 oleic acid, compound with (Z)-2-(heptade oleic acid, compound w 1g


 

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