Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :11940605
Publication Date : //

Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis.


Efficient endocytosis requires cytoplasmic domain targeting signals that specify incorporation of cargo into endocytic vesicles. Adaptor proteins play a central role in cargo collection by linking targeting signals to the endocytic machinery. We have characterized NPFX(1,2) (NPFX[1,2]D) targeting signals and identified the actin-associated protein Sla1p as the adaptor for NPFX(1,2)D-mediated endocytosis in Saccharomyces cerevisiae. 11 amino acids encompassing an NPFX(1,2)D sequence were sufficient to direct uptake of a truncated form of the pheromone receptor Ste2p. In this context, endocytic targeting activity was not sustained by conservative substitutions of the phenylalanine or aspartate. An NPFX1,2D-related sequence was identified in native Ste2p that functions redundantly with ubiquitin-based endocytic signals. A two-hybrid interaction screen for NPFX(1,2)D-interacting proteins yielded SLA1, but no genes encoding Eps15 homology (EH) domains, protein modules known to recognize NPF peptides. Furthermore, EH domains did not recognize an NPFX(1,2)D signal when directly tested by two-hybrid analysis. SLA1 disruption severely inhibited NPFX(1,2)D-mediated endocytosis, but only marginally affected ubiquitin-directed uptake. NPFX(1,2)D-dependent internalization required a conserved domain of Sla1p, SLA1 homology domain, which selectively bound an NPFX(1,2)D-containing fusion protein in vitro. Thus, through a novel NPF-binding domain, Sla1p serves as an endocytic targeting signal adaptor, providing a means to couple cargo with clathrin- and actin-based endocytic machineries.

Authors : Howard James P , Hutton Jenna L , Olson John M , Payne Gregory S ,

Related products :

Catalog number Product name Quantity
29-305 SRP14 belongs to the SRP14 family. The signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and 0.1 mg
EIAAB30630 Mouse,Mus musculus,Peroxin-5,Peroxisomal C-terminal targeting signal import receptor,Peroxisomal targeting signal 1 receptor,Peroxisome receptor 1,Pex5,PTS1 receptor,PTS1-BP,PTS1R,Pxr1,PXR1P
EIAAB30631 Homo sapiens,Human,Peroxin-5,Peroxisomal C-terminal targeting signal import receptor,Peroxisomal targeting signal 1 receptor,Peroxisome receptor 1,PEX5,PTS1 receptor,PTS1-BP,PTS1R,PXR1
EIAAB30627 Peroxin-5,Peroxisomal C-terminal targeting signal import receptor,Peroxisomal targeting signal 1 receptor,Peroxisome receptor 1,Pex5,Pex5_predicted,PTS1 receptor,PTS1-BP,PTS1R,Rat,Rattus norvegicus
EIAAB30629 Bos taurus,Bovine,Peroxin-5,Peroxisomal C-terminal targeting signal import receptor,Peroxisomal targeting signal 1 receptor,Peroxisome receptor 1,PEX5,PTS1 receptor,PTS1-BP,PTS1R,PXR1
EIAAB30628 Chicken,Gallus gallus,Peroxin-5,Peroxisomal C-terminal targeting signal import receptor,Peroxisomal targeting signal 1 receptor,Peroxisome receptor 1,PEX5,PTS1 receptor,PTS1-BP,PTS1R,RCJMB04_1g17
EIAAB11320 DnaJ homolog subfamily C member 13,DNAJC13,Homo sapiens,Human,KIAA0678,Required for receptor-mediated endocytosis 8,RME8,RME-8
25-604 Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three su 0.05 mg
29-272 Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three su 0.05 mg
26-803 Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for in 0.05 mg
28-734 AIP may play a positive role in AHR-mediated signalling possibly by influencing its receptivity for ligand and_or its nuclear targeting. AIP is the cellular negative regulator of the HBV X protein. 0.1 mg
28-333 AIP may play a positive role in AHR-mediated signalling possibly by influencing its receptivity for ligand and_or its nuclear targeting. AIP is the cellular negative regulator of the HBV X protein.AIP 0.1 mg
28-634 AIP may play a positive role in AHR-mediated signalling possibly by influencing its receptivity for ligand and_or its nuclear targeting. AIP is the cellular negative regulator of the HBV X protein. 0.1 mg
28-190 CREM is a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the s 0.1 mg
GWB-DC6393 Peroxisomal targeting signal 2 receptor
5257 Protein,Peroxisomal targeting signal 2 receptor 1 mg
E0380p Human ELISA Kit FOR Peroxisomal targeting signal 1 receptor 96T
CSB-EL017805RA Rat Peroxisomal targeting signal 1 receptor(PEX5) ELISA kit 96T
MLXPL_MOUSE Human ELISA Kit FOR Peroxisomal targeting signal 1 receptor 96T
CP27A_RAT Human ELISA Kit FOR Peroxisomal targeting signal 1 receptor 96T
CSB-EL017805GU Guinea pig Peroxisomal targeting signal 1 receptor(PEX5) ELISA kit 96T
CSB-EL017805CH Chicken Peroxisomal targeting signal 1 receptor(PEX5) ELISA kit 96T
CSB-EL017805HU Human Peroxisomal targeting signal 1 receptor(PEX5) ELISA kit 96T
CSB-EL017808HU Human Peroxisomal targeting signal 2 receptor(PEX7) ELISA kit 96T
CSB-EL017805RA Rat Peroxisomal targeting signal 1 receptor(PEX5) ELISA kit SpeciesRat 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur